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磷脂酰肌醇蛋白聚糖3(Glypican 3,GPC3)作为原发性肝癌标志物,对其结构与功能进行生物信息学分析。借助生物信息学相关软件,对该基因所编码的氨基酸理化性质、信号肽、跨膜结构、蛋白质的二级结构,蛋白质翻译后修饰、蛋白三级结构及亚细胞定位及B细胞线性表位、蛋白质相互作用网络进行生物信息学分析。GPC3蛋白为稳定的亲水性蛋白,具有信号肽,主要定位于细胞质膜。二级结构元件只要以α螺旋,β折叠为主。有18个Ser、7个Thr、23个Tyr可成为蛋白激酶磷酸化位点。GPC3蛋白与Wnt信号家族蛋白具有相互作用。分析预测GPC3,为探究其参与原发性肝癌方面有重要意义。
Abstract：GPC3 as an idiopathic biomarker of hepatocellular carcinoma, the study analyzed the bioinformatics of GPC3 gene structure and function. Physical and chemical properties, the signal peptide, transmembrane structure,protein secondary structure, protein posttranslational modification, protein super secondary structure and subcellular localization, B cell linear epitope, protein interaction networks of amino acid encoded by GPC3 were conducted bioinformatics analysis with the help of some bioinformatics software. GPC3 protein was the stable hydrophilic protein which had signal peptide, and was located in the plasma membrane. The main secondary structure elements were mainly α-helix and β-fold. It had 18 serines, 7 threonines and 23 tyrosines, which might become phosphorylation sites of the protein kinase. GPC3 protein interacted with Wnt signal family proteins. Analysis and prediction of GPC3 would be significantly important in exploring its involvement in hepatocellular carcinoma.
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基本信息:
DOI：10.13417/j.gab.037.001131
中图分类号:Q811.4;R735.7
引用信息:
[1]腾桥,夏丽洁,张富春.GPC3基因的结构与功能生物信息学预测[J].基因组学与应用生物学,2018,37(03):1131-1136.DOI:10.13417/j.gab.037.001131.
基金信息:
国家自然科学基金项目(No.31500752);; 新疆大学博士启动基金项目(No BS150241)共同资助
2018-03-25
2018-03-25