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为探究壬基酚(nonylphenol, NP)对异育银鲫(Carassius auratus gibelio)生长及脂质代谢的影响，将体重为(33.43±3.27)g的异育银鲫，分别浸浴在质量浓度为25、50和100μg/L的NP处理56 d后，测定异育银鲫生长性能及脂质代谢指标。结果显示∶(1)处理组异育银鲫末均重和肥满度(condition factor,CF)均显著降低(P <0.05),NP质量浓度为100μg/L时，异育银鲫质量增加率(weight gain rate,WGR)、特定生长率(specific growth rate,SGR)和存活率(survival rate,SR)均显著降低(P <0.05)，而50和100μg/L组的肝体指数(hepatosomatic index,HSI)和脏体指数(viscerasomatic index,VSI)显著增大(P <0.05);100μg/L组异育银鲫的肌肉粗脂肪(ether extract,EE)显著降低(P <0.05)，而肝脏EE显著升高(P <0.05)，其余各组差异不显著。(2)NP质量浓度为100μg/L时，异育银鲫血清甘油三酯(triglyceride,TG)明显降低而肝脏TG显著增高(P <0.05)，血清胆固醇(cholesterol,CHOL)显著增高而肝脏CHOL明显降低(P <0.05)。(3)NP质量浓度为50和100μg/L时，脂肪酶(lipase, LPS)、脂蛋白脂酶(lipoprotein lipase,LPL)活性显著降低(P <0.05),lpl基因的表达量显著升高(P <0.05)，而NP质量浓度为50μg/L时，肝脂酶(hepatic lipase, HL)活性明显增高(P <0.05),hl基因的表达量显著降低(P <0.05)。可见，当水体中NP浓度大于100μg/L时，异育银鲫生长受阻、SR降低，有关脂质代谢酶的活性降低，导致脂质代谢紊乱，从而对其生命活动带来危害。本研究结果可为研究NP对异育银鲫的毒性作用及脂肪肝的分子机制提供基础资料。
Abstract：Growth performance and lipid metabolism index were measured in gibel carp(Carassius auratus gibelio) with body weight of(33.43±3.27)g exposed to different concentrations(0, 25, 50 and 100 μg/L) of nonylphenol(NP) for 56 days. The results showed that:(1) The final average weight and condition factor(CF) of the treatment groups were significantly reduced(P < 0.05). The weight gain rate(WGR), specific growth rate(SGR)and survival rate(SR) were all significantly reduced only in high concentration group(NP, 100 μg/L)(P < 0.05).The hepatosomatic index(HSI) and viscerasomatic index(VSI) were significantly increased in medium(NP, 50 μg/L) and high concentration groups(P < 0.05). Muscle ether extract(EE) was significantly reduced, while liver ether extract was significantly increased in high concentration(P < 0.05). And there was no significant difference in the other groups.(2) Serum triglyceride(TG) and liver cholesterol(CHOL) were significantly reduced, while liver TG and serum CHOL were significantly increased in high concentration group(P < 0.05).(3) In medium and high concentration groups, lipase(LPS) and lipoprotein lipase(LPL) activities were significantly reduced, while the relative expression of lpl gene was significantly increased(P < 0.05). In medium concentration group, hepatic lipase(HL)activity was significantly increased, while the relative expression of hl gene was significantly reduced(P < 0.05).In summary, 100 μg/L NP can inhibit growth, reduce SR and activity of lipid metabolism enzymes. This study can provide basic data for future studies on the toxic effects of NP and the molecular mechanism of fatty liver.
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基本信息:
DOI：10.13417/j.gab.040.003418
中图分类号:S917.4;X503.225
引用信息:
[1]刘枝华,陈修云,董然然.壬基酚对异育银鲫(Carassius auratus gibelio)生长及脂质代谢的影响[J].基因组学与应用生物学,2021,40(Z4):3418-3425.DOI:10.13417/j.gab.040.003418.
基金信息:
贵州省科技厅联合基金资助项目(黔科合LH字[2015]7678); 贵州大学培育项目(黔科合平台人才[2017]5788-18)共同资助
2021-03-18
2021-03-18
2021-03-18