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杜仲为第三纪子遗树种,是我国特有的国家二级重点保护植物。贵州是杜仲的主产区之一,其多个地区均有栽培然而对其遗传背景尚不了解。本研究应用15个SSRs标记分析了贵州杜仲栽培群体的遗传多样性及亲缘关系,12个群体295个个体共检测到82个等位基因,海个位点的等位基因数为3~10,其均值为5.467;每个群体的等位基因数na、期望杂合度He、Shannon指数Ⅰ的平均值分别为4.011、0.533和0.996,多态位点百分率PPL除LL群体和QNY群体为93.3%外其余10个群体的PPL都为100%,说明贵州杜仲群体具有较高的遗传多样性;湄潭群体MT和龙里群体LL的固定指数(F)平均值为负值,其余都为正值表明群体内的杂合子缺失,群体内存在近亲繁殖现象;群体间的遗传分化系数FST为0.0660,遗传变异主要分布在群体内;UPGMA聚类分析发现,聚类分组并未按地区位置而聚类,这可能是在栽培过程中人为选择的结果,QNY群体与其他11个群体的遗传距离最大,XY群体和RH群体的遗传距离最近。本研究应用SSR分子标记探明其遗传多样性与亲缘关系,为杜仲的合理保护、种质资源收集提供建议。
Abstract：Eucommia ulmoides Olive is tertiary relict plant,and is the plant species that grows up only in China.It was listed the second-ranking protection category.Guizhou was one of the major E.ulmoides production areas,in which many areas were cultivated,but its genetic background wasn't still clear.In this study,we used 15 SSRs molecular markers to investigate the hereditary constitution and genetic relationship for the cultivated populations of E.ulmoides in Guizhou.The total of 82 alleles were detected based on 12 populations included 295 samples,the alleles per locus rang from 3 to 10,and average number was 5.467;the average number of alleles per population(no),expected heterozygosity(He),and Shannon's index(/) were 4.011,0.533 and 0.996,respectively.And except the percentage of polymorphic loci(PPL) of LL,QNY were 93.3%,the other 10 populations' PPL were 100%,indicated a higher level of genetic variation of E.ulmoides in Guizhou.Only the negative fixation index(F) exist in MT and LL,others were positive value,which implied heterozygote deficiency commonly existed in most populations,because of inbreeding within population.The genetic differentiation coefficient(FST) between populations were 0.066 0,suggested genetic variation mainly distributed within populations.The UPGMA cluster analysis indicated that grouping wasn't according to the religion location to cluster,it may be the result of artificial selection in the process of cultivation,QNY had the most genetic distance with other 11 populations.However,genetic distance between XY and RH was the closest.Some suggestions are recommended for both genetic conservation and germplasm collection of E.ulmoides.
Bauert M.R.,Klin M.,Baltisberger M.,and Edwards P.,1998,No genetic variation detected within isolated relict populations of Saxifraga cernua in the Alps using RAPD Markers,Molecular Ecology,7:1519-1527
Deng J.Y.,Liu Y.F.,and Huang H.W.,2006,Development and characterization of microsatellite markers in Eueommia ulmoides Oliver(Eucommiaceae),Molecular Ecology Notes,6(2):496-498
Dong W.,Huang S.X.,Chen R.,and Gui R.Y.,2010,Comparison on DNA extraction methods from betula alnoides leaves dried with silica gel,Xinan Linxueyuan Xuebao(Journal of Southwest Forestry University),30(4):36-38(董蔚,黄寿先,陈荣,桂仁意,2010,西南桦硅胶干燥叶DNA提取方法比较,西南林学院学报,30(4):36-38)
Forcioli D.,Saumitou L.P.,Valero M.,Vernet P.,and Cuguen J.,1998,Distribution of chloroplast DNA diversity within and among populations in gynodioecious Beta vulgaris ssp.,maritima(Chenopodiaceae),Molecular Ecology,7(9):1193-1204
Guo H.B.,Lu B.R.,Wu Q.H.,Chen J.K.,and Zhou T.S.,2007,Abundant genetic diversity in cultivated Codonopsis pilosula populations revealed by RAPD polymorphisms,Genetic Resources and Crop Evolution,54(5):917-924
Hamrick J.L.,and Godt M.W.,1990,Allozyme diversity in plant species,In:Brown A.H.,Clegg M.T.,Kahler A.,and Weir B.(eds.),Plant population genetics,breeding,and genetic resources.,Sinauer Associates Inc.,Sunderland.,Massachusetts,pp.43-63
Huang H.X.,Du H.Y.,Wuyun T.N.,and Zhu G.P.,2013.Establishment of genetic diversity system of eucommia ulmoides based on ssr molecular markers.,Linye Kexue Yanjiu(Forest Research),26(6):795-799(黄海燕,杜红宕,乌云塔娜,朱高浦,2013,基于SSR分子标记的杜仲遗传多样性体系建立,林业科学研究,26(6):795-799)
Kang C.Z.,Wang Q.Q.,Zhou T.,Jiang W.K.,Xiao C.H.,and Xie Y,2014,Study on ecological suitability regionalization of Eucommia ulmoides in Guizhou,Zhongyaocai(Journal of Chinese Medicinal Materials),37(5):760-766(康传志,王青青,周涛,江维克,肖承鸿,谢宇,2014,贵州杜仲的生态适宜性区划分析,中药材,37(5):760-766)
Owuor E.D.,Fahima T.,Beiles A.,Korol A.,and Nevo E.,1997,Population genetic response to microsite ecological stress in wild barley,Hordeum spontaneum,Molecular Ecology,6(12):1177-1187
Wang A.Q.,Huang L.Q.,Shao A.J.,Cui G.H.,Chen M.,and Dong C.H.,2006,Genetic diversity of Eucommia ulmoides by RAPD analysis,Zhongguo Zhongyao Zazhi(China Journal of Chinese Materia Medica),31(19):1583-1586(王暖琦,黄璐琦,邵爱娟,崔光红,陈敏,佟长辉,2006,子遗植物杜仲的遗传多样性RAPD分析和保护策略研究,中国中药杂志,31(19):1583-1586)
Wang B.M.,Wang Y.Q.,Mo H.,Luo L.X.,Li M.X.,and Cui K.M.,1999,Comparison of cytology,apical buds and gutta content between staminate and pistillate of Eucommia ulmoides trees,Zhiwu Xuebao(Acta Botanica Sinica),41(1):11-15(王丙武,王雅清,莫华,罗立新,李懋学,崔克明,1999,杜仲雌雄株细胞学,顶芽及叶含胶量的比较,植物学报,41(1):11-15)
Wang X.Y.,2013,Application of ISSRs to analysis of genetic diversity and sex identification for Eucommia ulmoides,Thesis for M.S.,Anhui Agricultural University,Supervisor:Cao C.P.,pp.3-16(王弦云,2013,ISSRs在杜仲遗传多样性研究及性别鉴定中的应用,硕士学位论文,安徽农业大学,导师:曹翠萍,pp.3-16)
Wang Z.F.,and Peng S.L.,2003,Plant conservation genetics,Shengtai Xuebao(Acta Ecologica Sinica),23(1):159-173(王峥峰,彭少麟,2003,植物保护遗传学,生态学报,23(1):159-173)
Wu M.,2014,Development of genomic SSR makers and genetic diversity evaluation of Eucommia ulmoides Oliv.,Thesis for M.S.,Chinese Academy of Forestry,Supervisor:Du H.Y.,pp.10-15(吴敏,2014,杜仲全基因组SSR标记开发及遗传多样性评价,硕士学位论文,中国林业科学研究院,导师:杜红宕,pp.10-15)
Wu M.,Du H.X.,Wu Y.T.N.,Liu P.F.,and Jing T.,2015,Characterization of genomic microsatellites and development ofSSR markers of Eucommia ulmoides,Linye Kexue Yanjiu(Forest Research),28(3):387-393(吴敏,杜红宕,乌云塔娜,刘攀峰,荆腾,2015,杜仲基因组微卫星特征及SSR标记开发,林业科学研究,28(3):387-393)
Wu M.Q.,Chen S.L.,Wang M.X.,and Yao S.Z.,2011,An analysis of the genetic diversity and genetic structure of Eucommia ulmoides using inter-simple sequence repeat(ISSR)markers,African Journal of Biotechnology,10(84):19505-19513
Xiao C.H.,Zhou T.,Jiang W.K.,Deng D.M.,Zhao D.,and Xiong H.X,2014,Analysis of genetic diversity of cultivated Eucommix ulmoides from Guizhou province by phenotypic traits and SCOT markers,Zhongyaocai(Journal of Chinese Medicinal Materials),37(8):1342-1348(肖承鸿,周涛,江维克,邓冬梅,赵丹,熊厚溪,2014,贵州栽培杜仲表型性状与SCOT分子标记的遗传多样性分析,中药材,37(8):1342-1348)
Yan C.Y.,Li J.X.,He Y.M.,Li B.,and Huang H.L.,2014,Genetis diversity of 30 Camellia oleifera germplasms by using SRAP Marker,Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),33(3):617-623(晏嫦好,李家贤,何玉媚,李波,黄华林,2014,利用SRAP标记分析30份油茶种质资源的遗传多样性,基因组学与应用生物学,33(3):617-623)
Yao X.,Deng J,and Huang H.,2012,Genetic diversity in Eucommix ulmoides(Eucommiaceae),an endangered traditional Chinese medicinal plant,Conservation Genetics,13(6):1499-1507
Yu J.,Wang Y.,Peng L.,Ru M.,Liang Z.,2015a,Genetic diversity and population structure of Eueommia ulmoides Oliver,an endangered medicinal plant in.China,Genetics and Molecular Research,14(1):2471-2483
Yu J.,Wang Y.,Ru M.,Peng L.,and Liang Z.,2015b.,Genetic diversity in intraspecific hybrid populations of Eucammia ulmoides Oliver evaluated from ISSR and SRAP molecular marker analysis,Genetics and Molecular Research,14(3):7417-7425
Zhang B.Y.,Zhang K.T.,Wang Y.Q.,and Bai M.S.,2003,The selection of fine germplasm regions and variant types of Eucommia,Xibei Linxueyuan Xuebao(Journal of Northwest Forestry University),18(4):32-34(张博勇,张康健,王亚琴,白明生,2003,杜仲优良种源区与类型选择的研究,西北林学院学报,18(4):32-34)
Zhang J.J.,Xing C.,Tian H.,and Yao X.H.,2013,Mierosatellite genetic variation in the Chinese endemic Eucommia ulmoides(Eucommiaceae):implications for conservation,Botanical Journal of the Linnean Society,173(4):775-785
Zhu X.M.,2013,Genetic diversity of Eucommia ulmoides in Anhui province based on ISSRs,Thesis for M.S.,Anhui Agri cultural University,Supervisor:Cao C.P.,pp.3-7(朱晓敏,2013,基于ISSRs的安徽省杜仲资源的遗传多样性研究,硕士学位论文,安徽农业大学,导师:曹翠萍,pp:3-7)
Zinck J.W.,and Rajora O.R.,2016,Post-glacial phylogeography and evolution of a wide-ranging highly-exploited keystone forest tree,eastern white pine(Pines strobes)in North America:single refugium,multiple routes,BMC Evolutionary Biology,16:56
基本信息:
DOI：10.13417/j.gab.035.001521
中图分类号:S567.19
引用信息:
[1]林开勤,李岩,邹冰杰,等.贵州杜仲栽培群体遗传多样性及亲缘关系的SSR分析[J].基因组学与应用生物学,2016,35(06):1521-1529.DOI:10.13417/j.gab.035.001521.
基金信息:
国家863计划课题“杜仲功能基因组研究与应用”(2013AA102605);; 贵州大学研究生创新基金(研农2015011)共同资助
2016-06-25
2016-06-25