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本研究旨在比较不同生长阶段的水牛(Bubalus bubalis)血清生化指标以及血清代谢物，以期发掘差异血清生化成分和差异代谢标志物。随机采集30头不同生长阶段(10头犊牛哺乳期、 10头断奶期、 10头成年期)的水牛血清样本，分别用于测定血清生化指标和血清内源代谢物。研究结果表明，3个生长阶段之间均有显著差异(两两对比P<0.05)的指标分别是尿素氮、甘油三酯、免疫球蛋白A、总胆固醇和间接胆红素。与成年期水牛相比，犊牛哺乳期水牛总胆固醇水平较低，因为瘤胃吸收的醋酸盐丰度较低和对糖异生的依赖程度较低。血清代谢组比较分析发现，在正负离子模式下分别鉴定出4 824种和11 522种代谢物，从3个生长阶段两两比较后的差异代谢物中筛选到12种血清差异代谢物，包括1,3,7-三甲基尿酸、可可碱、甲基黄嘌呤、尿囊酸、胞苷、 3-尿苷丙酸和花生四烯酸等。在不同生长阶段的水牛之间主要在氨基酸和脂肪酸合成代谢通路存在显著差异，说明在水牛犊牛哺乳期向断奶期过渡时，蛋白质代谢途径显著提高，因此建议在基础日粮里适量添加蛋白质，旨在保障水牛健康生长，提高水牛对饲料的利用效率。成年水牛血清中的甲基黄嘌呤和色氨酸含量高，说明成年水牛相较幼年水牛抗氧化能力和免疫能力显著提高。
Abstract：The aim of this study was to compare serum biochemical indices as well as serum metabolites in buffaloes(Bubalus bubalis)at different growth stages with a view to discovering differential serum biochemical components and differential metabolic markers.Serum samples were randomly collected from 30 buffaloes at different growth stages(10 breastfeeding, 10 weaning and 10 adulthood)and used for the determination of serum biochemical indices and serum endogenous metabolites. The results showed that the biochemical indices that were significantly different(P<0.05 for two-by-two comparison) among the three growth stages were urea nitrogen, triglycerides, immunoglobulin A, total cholesterol, and indirect bilirubin, respectively. Total cholesterol levels were lower in breastfeeding buffaloes compared to adult buffaloes due to lower abundance of rumen absorbed acetate and less dependence on gluconeogenesis. Comparative analysis of the serum metabolome revealed that 4 824 and 11 522 metabolites were identified in positive and negative ion modes, respectively. Among the differential metabolites compared pairwise in three growth stages, 12 serum differential metabolites were selected, including 1,3,7-trimethyluric acid, theobromine, methylxanthine, allantoic acid, cytidine, 3-uridine propionic acid, and arachidonic acid. During the growth process of buffalo, there were significant differences in the synthesis and metabolism pathways of amino acid and fatty acid, indicating that the protein metabolic pathways were significantly increased during the transition from breastfeeding to weaning in buffaloes. Therefore, it is recommended to add moderate amounts of protein to basal diets aimed at safeguarding the healthy growth of buffaloes and improve the efficiency of utilization of feed.The high levels of methylxanthine and tryptophan in the serum of adult buffaloes indicate a significant increase in the antioxidant and immune capacity of adult buffaloes compared to young buffaloes.
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基本信息:
DOI：10.13417/j.gab.043.001565
中图分类号:S823.83
引用信息:
[1]刘淼儿,丁子旭,徐祎雪,等.不同生长阶段水牛血清代谢物差异比较研究[J].基因组学与应用生物学,2024,43(Z1):1565-1574.DOI:10.13417/j.gab.043.001565.
基金信息:
国家自然科学基金项目(U20A2051); 广西科技重大专项(桂科AA2361)共同资助
2024-06-01
2024-06-01
2024-06-01