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本研究以连香树未成熟种子子叶胚诱导产生的胚性愈伤组织与非胚性愈伤组织为原材料,测定了两者的可溶性蛋白质、可溶性糖及游离脯氨酸的含量,并利用非变性聚丙烯酰胺凝胶电泳技术(polyacrylamide gelelectrophoresis, PAGE)对两者的过氧化物酶(peroxidase, POD)、超氧化物歧化酶(superoxide dismutase,SOD)、淀粉酶(amylase, AMY)同工酶进行了分析。比较发现胚性愈伤组织在可溶性蛋白质和游离脯氨酸的含量上均极显著高于非胚性愈伤组织。在可溶性糖含量上,胚性愈伤组织也显著地高于非胚性愈伤组织。胚性愈伤组织具有较高的代谢活性,为进一步胚性分化提供物质和能量基础。POD、SOD和AMY同工酶在连香树胚性愈伤组织与非胚性愈伤组织中酶带差异明显,表明这3种酶的活性与胚性愈伤组织的诱导密切相关,酶谱的差异可作为体细胞胚胎发生的参考标记。
Abstract：The contents of soluble protein, soluble sugar and free proline in embryogenic callus and non-embryogenic callus induced by immature cotyledon embryos of Cercidiphyllum japonicum were determined. And non-denaturing polyacrylamide gel electrophoresis was used to analyze isozyme patterns such as peroxidase(POD),superoxide dismutase(SOD) and amylase(AMY) in the two types of calli. By comparison, it is shown that the contents of soluble protein and free proline of embryogenic callus were very significantly higher than those in non-embryogenic callus. As for soluble sugar content, it was significantly higher in embryogenic callus than non-embryogenic callus. The higher metabolic activity of embryogenic callus provided the basis of material and energy for further embryonic differentiation. The isozyme strands analysis of POD, SOD and AMY showed significant differences in embryogenic callus and non-embryogenic callus, which shared a close relationship in the induction of embryogenic callus. The differences between isozyme strands can be a reference label of the Somatic Embryogenesis.
Chen R.Z.,Shen Y.B.,Luo X.F.,and Zhang J.Q.,2012,Research on tissue culture and plant regeneration of Cercidiphyllum japonicum,Heilongjiang Nongye Kexue(Heilongjiang Agricultural Sciences),(1):16-21(陈荣珠,沈应柏,罗晓芳,张俊琦,2012,连香树组织培养及植株再生体系的初步研究,黑龙江农业科学,(1):16-21)
Cui K.R.,and Dai R.L.,eds.,2000,Molecular biology of plant somatic embryogenesis,Science Press,Beijing,China,pp.48-57(崔凯荣,戴若兰,植物体细胞胚发生的分子生物学,2000,科学出版社,中国,北京,pp.48-57)
Fransz P.F.,de Ruijter N.C.A.,and Schel J.H.N.,1989,Isozymes as biochemical and cytochemical markers in embryogenic callus cultures of maize(Zea mays L.),Plant Cell Reports,8(2):67-70
Gao J.F.,ed.,2006,Experimental guidance for plant physiology,Higher Education Press,Beijing,China,pp.140-144(高俊凤,编著,2006,植物生理学实验指导,高等教育出版社,中国,北京,pp.140-144)
He Z.X.,and Zhang S.Z.,eds.,1999,Electrophoresis,Science Press,Beijing,China,pp.37-38,286-300(何忠效,张树政,编著,1999,电泳,科学出版社,中国,北京,pp.37-38,286-300)
Hong D.,Sun F.C.,Ji Y.K.,Gao W.J.,Deng C.L.,and Lu L.D.,2008,Comparatively analysis on isozyme patterns of Asparagus of fcinalis L.male and female plants,Henan Shifandaxue Xuebao(Journal of Henan Normal University)(Natural Science Edition),36(1):111-114(洪达,孙富从,姬艳克,高武军,邓传良,卢龙斗,2008,芦笋雌雄性别间同工酶酶谱的比较分析,河南师范大学学报(自然科学版),36(1):111-114)
Jiang C.R.,Peng F.R.,and Tan P.P.,2014,Somatic embyrogenesis and the physiological and biochemical characteristics in Catalpa fargesii Bur.f.duclouxii(Dode)Gilmour,Linye Keji Kaifa(China Forestry Science and Technology),28(1):25-29(江翠荣,彭方仁,谭鹏鹏,2014,滇楸体胚发生及生理生化特性研究,林业科技开发,28(1):25-29)
Li F.G.,Li X.L.,and Li F.L.,1994,Changes of major biochemical metabolites in somatic embryogenesis of cotton,Henan Nongyedaxue Xuebao(Journal of Henan Agricultural University),28(3):313-316(李付广,李秀兰,李凤莲,1994,棉花体细胞胚胎发生及主要物质生化代谢机制,河南农业大学学报,28(3):313-316)
Li Q.,Zhang C.X.,and Qin P.,2008,Physiological and biochemical characteristics of embryogenic callus and non-embryogenic callus in Pinus bungeana,Xibei Nonglinkejidaxue Xuebao(Journal of Northwest A&F University)(Natural Science Edition),36(8):151-154(李茜,张存旭,秦萍,2008,白皮松胚性和非胚性愈伤组织生理生化特性研究,西北农林科技大学学报(自然科学版),36(8):151-154)
Lin Y.L.,2011,Studies on cloning,expression and regulation of SOD gene family during somatic embryogenesis in Dimocarpus longan Lour.,Dissertation for Ph.D.,Fujian Agriculture and Forestry University,Supervisor:Lai Z.X.,pp.32(林玉玲,2011,龙眼体胚发生过程中SOD基因家族的克隆及表达调控研究,博士学位论文,福建农林大学,导师:赖钟雄,pp.32)
Meng J.,Zhang C.X.,Sun L.,and Rong X.X.,2015,Biochemical characteristic variations of embryogenic and non-embryogenic callus of Quercus variabilis,Xibei Linxueyuan Xuebao(Journal of Northwest Forestry University),30(5):106-110(孟姣,张存旭,孙琳,戎旭旭,2015,栓皮栎胚性和非胚性愈伤组织生化特性研究,西北林学院学报,30(5):106-110)
Neves N.,Segura-Nieto M.,Blanco M.A.,Sánchez M.,González A.,González J.L.,and Castillo R.,2003,Biochemical characterization of embryogenic and non-embryogenic calluses of sugarcane,In Vitro Cellular&Development-Plant,39(3):343-340
Wei H.Y.,2016,The study of somatic embrygenesis of regeneration in Cerdiphyllum japonicum Sieb.,Thesis for M.S.,Guangxi University,Supervisor:Li Z.L.,pp.14,19-20(韦虹宇,2016,连香树体细胞胚胎发生及植株再生体系的研究,硕士学位论文,广西大学,导师:李在留,pp.14,19-20)
Xia M.X.,Wang W.,Yuan R.,Deng F.N.,and Shen F.F.,2015,Superoxide dismutase and its research in plant stress-tolerance,Fenzi Zhiwu Yuzhong(Molecular Plant Breeding),13(11):2633-2646(夏民旋,王维,袁瑞,邓粉妮,沈法富,2015,超氧化物歧化酶与植物抗逆性,分子植物育种,13(11):2633-2646)
Xin F.M.,Zhang C.X.,and Wang H.,2007,Antioxides characteristics of embryogenic callus and non-embryogenic callus in Quercus variabilis,Xibei Linxueyuan Xuebao(Journal of Northwest Forestry University),22(3):67-70(辛福梅,张存旭,王红,2007,栓皮栎胚性和非胚性愈伤组织抗氧化物酶的研究,西北林学院学报,22(3):67-70)
Xin W.J.,2006,Somatic embryogenesis and physiological and biochemical characteristics of anthurium,Thesis for M.S.,Nanjing Agricultural University,Supervisors:Wang G.D.,and Guo W.M.,pp.44(辛伟杰,2006,花烛体细胞胚胎发生及相关生理生化研究,硕士学位论文,南京农业大学,导师:王广东,郭维明,pp.44)
Xiong D.,Chen F.J.,Liang H.W.,Wang Y.B.,and Li X.L.,2008,Studies on four kinds of isozymes during somatic embryogenesis of Emmenopterys henryi Oliv.,Xibei Zhiwu Xuebao(Acta Botanica Boreali-Occidentalia Sinica),28(10):2011-2016(熊丹,陈发菊,梁宏伟,王玉兵,李晓玲,2008,香果树体细胞胚胎发生过程中4种同工酶的研究,西北植物学报,28(10):2011-2016)
Yuan L.J.,2008,Tissue culture and rapid propagation of rare and precious species Cercidiphyllum japonicum,Thesis for M.S.,Agricultural University of Hebei Province,Supervisor:Ye Y.Z.,pp.29-30(袁丽洁,2008,珍稀植物连香树组织培养技术体系研究,硕士学位论文,河北农业大学,导师:叶永忠,pp.29-30)
Zhang B.,Jing D.L.,Li X.L.,Wang Y.B.,and Zhang D.C.,2011,Studies on isozymes during somatic embryogenesis of Tapiscia sinensis,Guangxi Zhiwu(Guihaia),31(4):526-530(张博,景丹龙,李晓玲,王玉兵,张德春,2011,珍稀濒危植物银鹊树体细胞胚时期同工酶分析,广西植物,31(4):526-530)
Zhang X.L.,Sun J.Z.,and Liu J.L.,1992,A comparative study on the biochemical metabolites between the embryogenic and non-embryogenic calli from the variety“coker 201”of Gossypium hirsutum L.,Zuowu Xuebao(Acta Agronomica Sinica),18(3):176-181(张献龙,孙济中,刘金兰,1992,陆地棉品种“珂字201”胚性与非胚性愈伤组织生化代谢产物的比较研究,作物学报,18(3):176-181)
基本信息:
DOI：10.13417/j.gab.037.004449
中图分类号:S792.99
引用信息:
[1]王慧纯,韦虹宇,刘金炽,等.连香树胚性与非胚性愈伤组织生理生化差异及同工酶分析[J].基因组学与应用生物学,2018,37(10):4449-4454.DOI:10.13417/j.gab.037.004449.
基金信息:
国家“十二五”科技支撑计划课题(2012BAD21B03)项目资助
2017-08-10
2017-08-10
2017-08-10