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本研究利用限制性内切酶片段长度多态性(RFLP)和16SrDNA序列分析相结合的方法研究了广西南宁高峰林场人工桉树林中土壤微生物的群落结构。通过采用直接法提取桉树人工林土壤中微生物总DNA,构建细菌的l6SrDNA克隆文库,从中随机挑取了192个阳性克隆进行检测,分析显示188个阳性克隆子确定含有16SrDNA并分归为52个不同的类群OTUs,随机挑取其中35个克隆进行测序,并构建了系统发育进化树。研究结果表明:桉树人工林土壤中细菌种类较为丰富,含有大量的未培养微生物,进一步的序列分析表明桉树人工林土壤中占优势的类群分别为A cidobacteria(40%)和Proteobacteria(27%)。本研究对桉树人工林土壤微生物群落结构进行了研究,为进一步研究桉树人工林环境质量变化提供基础资料。
Abstract：In this research, we investigated the edaphon community structure from Gaofeng forest-farm Eucalyptus plantation in Guangxi Nanning by PCR-RFLP technique and 16S rDNA sequence analysis. Total DNA of microor- ganism in Eucalyptus plantation was extracted by using the direct method, then we constructed a 16S rDNA clone library of the soil microbes. A total of 192 positive clones were radomly chosen from the clone library to be examined. The PCR-RFLP result showed that 188 positive clones determinately contained 16S rDNA and they went back to 52 different operational taxonomic units (OTUs). We also radomly chose 35 clones among them to sequence and constructed phylogenetic development tree. The results indicated that the bacteria species in Eucalyptus plantation soil are relatively abundance. Further sequence analysis showed that the dominant bacteria in this Eucalyptus plantation soil were Acidobacteria and Proteobacteria which was 40% and 27%, respectively. This resarech investigated the edaphon community structure of Eucalyptus plantation, which would provide the foundation information for studying the variety in environmental quality of Eucalyptus plantation.
Barns S.M.,Takala S.L.,and Kuske C.R.,1999,Wide distribu-tion and diversity of members of the bacterial kingdom Aci-dobacterium in the environment,Appl.Environ.Microbiol.,65(4):1731-1737
Dong X.Z.,and Cai M.Y.,eds.,2001,Manual of systematic anddeterminative bacteriology,Science Press,Beijing,China,pp.399-412(东秀珠,蔡妙英,编,2001,常见细菌系统鉴定手册,科学出版社,中国,北京,pp.399-412)
Hill T.C.J.,Walsh K.A.,Harris J.A.,and Moffett B.,2003,Usingecological diversity measures with bacterial communities,FEMS Microbiol.Ecol.,43(1):1-11
Huang Q.G.,Tian B.Y.,Jiang X.Z.,Ke C.R.,and Huang J.Z.,2009,Community construction and function of soil bacteriain a forest decomposed-hollow stump environment,Sheng-wu Jishu Tongbao(Biotechnology Bulletin),3:132-136(黄钦耿,田宝玉,江贤章,柯崇榕,黄建忠,2009,福州森林红壤细菌的菌群结构及其功能分析,生物技术通报,3:132-136)
Jiang J.L.,Zhou Q.N.,Che Z.Q.,Deng Z.Q.,and Wu B.,2008,Bacterial diversity in household biogas digester sludge ana-lyzed by PCR-RFLP,Guangxi Nongye Shengwu Kexue(Journal of Guangxi Agricultural and Biological Science),27(4):372-377(蒋建林,周权能,车志群,邓珍琴,武波,2008,PCR-RFLP技术分析沼气池厌氧活性污泥细菌的多样性,广西农业生物科学,27(4):372-377)
Liu Y.L.,Lv Q.F.,Pan S.M.,and Yuan H.X.,2006,Misroorgan-ism isolated from litters of the stands of Eucalyptus,NanjingLinye Daxue Xuebao(Journal of Nanjing Forestry Universi-ty),30(1):75-78(刘月廉,吕庆芳,潘颂民,袁红旭,2006,桉树林分枯落物分解微生物的种类和数量,南京林业大学学报,30(1):75-78)
Schwudke D.,Bernhardt A.,Beck S.,Madela K.,Linscheid M.W.,Appel B.,and Straunch E.,2005,Transcriptional activity ofthe host-interaction locus and a putative pilin gene ofbdellovibrio bacteriovorus in the predatory life cycl,CurrentMicrobiol.,51(5):310-316
von Wintzingerode F.,GobeJ U.B.,and Stackebrandt E.,1997,Determination of microbial diversity in environmental sam-ples:pitfalls of PCR-based rRNA analysis,FEMS Micmbiol.Rev.,21(3):213-229
Wen Y.G.,Liu S.R.,and Chen F.,2005,Effects of continuouscropping on understorey species diversity in eucalypt plan-tations,Yingyong Shengtai Xuebao(Chinese Journal of Ap-plied Ecology),16(9):1667-1671(温远光,刘世荣,陈放,2005,连栽对桉树人工林下物种多样性的影响,应用生态学报,16(9):1667-1671)
Xia B.C.,Zhou J.,and Tiedje J.M.,2001,Structures of bacteriacloning communities in the soil environment and their eco-logical characteristics,Shengtai Xuebao(Acta Ecologica Sni-ca),21(4):574-578(夏北成,Zhou J.,Tiedje J.M.,2001,土壤细菌类克隆群落及其结构的生态学特征,生态学报,21(4):574-578)
Zhou J.,Bruns M.A.,and Tiedje J.M.,1996,DNA recovery fromsoils of diverse composition,Appl.Envir.Microbiol.,62(2):316-22
Zhao Y.H.,Yang Y.M.,Yang S.Y.,and Wang J.,2007,A reviewof the biodiversity in Eucalyptus plantation,Yunnan NongyeDaxue Xuebao(Journal of Yunnan Agricultural University),22(5):741-746(赵一鹤,杨宇明,杨时宇,王娟,2007,桉树人工林生物多样性研究进展,云南农业大学学报,22(5):741-746)
基本信息:
中图分类号:S714
引用信息:
[1]段思蒙,申佩弘,潘莉莉,等.PCR-RFLP分析桉树人工林土壤微生物的群落结构[J].基因组学与应用生物学,2009,28(05):859-864.
2009-10-28
2009-10-28