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硒是人和动物必需的微量元素,不同浓度的硒可以调节猪、羔羊、小鼠、人和酵母中与脂质和葡萄糖代谢相关基因的表达水平,但是目前尚不清楚低剂量硒对HepG2细胞脂质和葡萄糖代谢相关基因的影响。因此,我们通过实时荧光定量PCR和基因组学分析,研究了亚硒酸钠对HepG2细胞中脂质和葡萄糖代谢相关基因表达水平的影响。结果表明,脂质代谢相关基因FAR1,DGKD和HILPDA在24 h和36 h时表达增加,葡萄糖代谢相关基因UGDH,IRS-2和PEPCK在24 h和36 h时表达增加。与对照组相比,0.1μmol/L亚硒酸钠处理HepG2细胞24 h可以增加TRAP1,HILPDA和PEPCK的表达水平,它们在肿瘤发生发展中起着重要的作用。此外,转录组学分析表明,亚硒酸钠可以改变代谢调控网络,包括萜类骨架的生物合成、胰岛素抵抗、磷酸肌醇代谢和细胞周期途径等。以上结果表明,0.1μmol/L亚硒酸钠可能会影响HepG2细胞的脂质和葡萄糖代谢,影响肿瘤的发生发展。
Abstract：Selenium, as a necessary trace element for humans and animals, can regulate the levels of lipid and glucose metabolism related genes in pigs, lambs, mice, humans and yeast at different concentrations. However, the effect of low dose of selenium on lipid and glucose metabolism in HepG2 cell is unclear, The real time PCR and genomics analysis were performed to evaluate the changes of lipid and glucose metabolism related genes in sodium selenite treated HepG2 cells. The results suggested that the lipid metabolism related genes, FAR1, DGKD and HILPDA, increased at 24 h or 36 h, and the glucose metabolism related genes, UGDH, IRS-2 and PEPCK, also increased at 24 h and 36 h, compared with those at 0 h. And 0.1 μmol/L sodium selenite could only increase the expression levels of TRAP1, HILPDA and PEPCK at 24 h, which of them play an important role in tumor development. Moreover, transcriptomics analysis suggested that selenium treatment could change the metabolic regulatory network, including terpenoid backbone biosynthesis, indicated resistance, inositol phosphate metabolism and cell cycle pathways. The above results indicate that 0.1 μmol/L sodium selenite could influence lipid and glucose metabolism in HepG2 cells.
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基本信息:
DOI：10.13417/j.gab.040.001838
中图分类号:Q75
引用信息:
[1]张亮亮,易婉婷,黄江涛,等.亚硒酸钠对HepG2细胞脂质和葡萄糖代谢相关基因表达的影响[J].基因组学与应用生物学,2021,40(04):1838-1845.DOI:10.13417/j.gab.040.001838.
基金信息:
国家自然科学基金项目(21561006; 21867007); 贵州省科学技术基金项目([2019]1258号; LH-[2016]7372号)共同资助
2020-06-09
2020-06-09
2020-06-09