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随着不可再生资源的迅速减少,生物化工产业的迅速发展,人们对乙醇的需求量越来越大。单一化学合成法生产的乙醇中夹杂了异构高碳醇,对人体有害,不适宜食品和医药行业的应用。因此,生产大量的对人体无害的乙醇仍然是一项热门研究领域。Jxnu-18是一株乙醇生产菌株。解析Jxnu-18菌株的基因组序列信息,可以深入研究Jxnu-18菌株产生乙醇的分子代谢机制,为以后该菌株的分子改造育种研究提供相应的基因序列信息。本研究通过对Jxnu-18菌株进行全基因组测序,并应用相关软件对测序数据进行序列组装、功能注释、GO/COG聚类分析等。结果表明Jxnu-18菌株的全基因组大小为4 059 815 bp,GC含量为46.14%,菌株的基因序列已提交至GenBank数据库,Accession编号为CNSA:CNP0000014;ENA:PRGEB23600。
Abstract：With the rapid decline of non-renewable resources and the rapid development of the biochemical industry, the demand for ethanol is increasing. Ethanol produced by a single chemical synthesis method is contaminated with harmful high-carbon alcohols, and is not suitable for use in industries such as food and medicine. Therefore, the production of a large amount of ethanol that is harmless to the human body is still a hot research field. Jxnu-18 is an ethanol production strain. The analysis of the genome sequence information of Jxnu-18 strain can further study the molecular metabolism mechanism of ethanol produced by Jxnu-18 strain and provide corresponding sequence background information for molecular breeding research. In this study, the whole genome sequencing of Jxnu-18 strain was carried out, and the sequencing software was used for sequence assembly, functional annotation, GO/COG cluster analysis and so on. The results showed that the whole genome size of Jxnu-18 strain was 4 059 815 bp and the GC content was 46.14%. The gene sequence of the strain was submitted to GenBank database. Accession number was CNSA: CNP0000014; ENA: PRGEB23600.
Akinosho H.,Yee K.,Close D.,and Ragauskas A.,2014,The emergence of Clostridium thermocellum as a high utility candidate for consolidated bioprocessing applications,Front.Chem.,2:66
Almarsdottir A.R.,Sigurbjornsdottir M.A.,and Orlygsson J.,2012,Effect of various factors on ethanol yields from lignocellulosic biomass by Thermoanaerobacterium AK17,Biotechnol.Bioengineering,109(3):686-694
Delcher A.L.,Bratke K.A.,Powers E.C.,and Salzberg S.L.,2007,Identifying bacterial genes and endosymbiont DNA with Glimmer,Bioinformatics,23(6):673-679
Kanehisa M.,and Goto S.,2000,KEGG:Kyoto encyclopedia of genes and genomes,Nucleic Acids Res.,27(1):29-34
Lagesen K.,Hallin P.,Rdland E.A.,Strfeldt H.H.,and Rognes T.,2007,Ussery DW.RNAmmer:Consis tent and rapid annotation of ribosomal RNA genes,Nucleic Acids Res.,35(9):3100-3108
Li W.H.,2011,Preliminary study on the production of alcohol by bamboo cellulose microbial fermentation,Thesis for M.S.,Jiangxi Agricultural University,Supervisor:Tu G.Q.,pp.3-6(李文华,2011,竹质纤维素微生物发酵生产酒精工艺的初步研究,硕士学位论文,江西农业大学,导师:涂国全,pp.3-6)
Li X.M.,and Deng Y.,2005,Alcohol abuse in the injury of central nervous system,Zhongguo Zuzhi Gongcheng Yanjiu(Chinese Journal of Tissue Engineering Research),9(21):181-183(李秀敏,邓源,2005,乙醇的中枢神经系统损伤作用,中国组织工程研究,9(21):181-183)
Liang Y.N.,and Li B.,2011,Enzymatic characteristics of alcohol dehydrogenase of persimmon fruit,Zhongguo Nongye Daxue Xuebao(Journal of China Agricultural University(Social Sciences Edition)),16(5):65-70(梁银娜,李宝,2011,柿果实乙醇脱氢酶酶学特性研究,中国农业大学学报(社会科学版),16(5):65-70)
Liu X.,and He G.Q.,2003,Microbial metabolism engineering for the production of fuel ethanol from lignocellulose,Liangyou Jiagong yu Shiping Jixie(Grain and Oil Processing and Food Machinery),(8):67-69(刘翔,何国庆,2003,利用木素纤维素生产燃料乙醇的微生物代谢工程,粮油加工与食品机械,(8):67-69)
Lowe T.M.,and Eddy S.R.,1997,t RNAscan-SE:a program for improved detection of transfer RNA genes in genomic sequence,Nucleic Acids Res.,25(5):955-964
Mistry F.R.,and Cooney C.L.,1989,Production of ethanol by Clostridium thermosaccharolyticum:I.Effect of cell recycle and environmental parameters,Biotechnology and Bioengineering,10(5):6-21
Tatusov R.L.,Galperin M.Y.,and Natale D.A.,2000,The COGdatabase:A tool for genome-scale analysis of protein functions and evolution,Nucleic Acids Res.,28(1):33-36
Wang W.,Wang H.,Meng C.,and Guo Y.H.,2007,Study of breeding Saccharomyces cerevisiae with improved temperature and ethanol tolerance by genome shuffling,Weishengwuxue Tongbao(Microbiology China),34(4):705-708(王灏,王航,孟春,郭养浩,2007,基因组改组技术选育耐高温、耐高乙醇酿酒酵母菌株的研究,微生物学通报,34(4):705-708)
Xu X.,2001,106 Virus-mediated hammerhead R enzyme inhibits the expression of pyruvate-ferredoxin oxidoreductase gene in Giardia,Guoji Yixue Jishengchongbing Zazhi(International Journal of Medical Parasitic Diseases),28(3):140-141(徐霞,2001,106病毒介导的锤头状R酶抑制贾第鞭毛虫丙酮酸-铁氧化还原蛋白氧化还原酶基因表达,国际医学寄生虫病杂志,28(3):140-141)
Yang D.F.,Wei Y.T.,Zhou X.,Huang Z.M.,and Huang R.B.,2006,Cloning,expression and characterization of pyruvate formate-lyase in Escherich coli,Zhongguo Shengwu Gongcheng Zazhi(China Biotechnology),26(8):28-31(杨登峰,韦宇拓,周兴,黄志民,黄日波,2006,丙酮酸甲酸裂解酶基因在大肠杆菌中的克隆、表达及酶学性质的研究,中国生物工程杂志,26(8):28-31)
Zheng J.L.,Zhu X.F.,Guo Q.X.,and Zhu Q.S.,2005,An overview of technology for liquid fuel from biomass,Zhongguo Gongcheng Kexue(Engineering Sciences),7(4):5-10(郑冀鲁,朱锡锋,郭庆祥,朱清时,2005,生物质制取液体燃料技术发展趋势与分析,中国工程科学,7(4):5-10)
Zhu B.Y.,and Li H.M.,2010,Reviews on pyruvate decarboxylase and its application,Shengming Kexue(Biology),(11):1184-1191(朱碧云,李浩明,2010,丙酮酸脱羧酶及其应用研究,生命科学,(11):1184-1191)
基本信息:
DOI：10.13417/j.gab.039.003490
中图分类号:TQ920.1;TQ223.122
引用信息:
[1]张菊,杜思瑶,方琳琳,等.一株从豆豉中分离的乙醇生产菌株Jxnu-18的全基因组分析[J].基因组学与应用生物学,2020,39(08):3490-3495.DOI:10.13417/j.gab.039.003490.
基金信息:
国家自然科学基金(31760449);; 江西省科技厅支撑计划项目(20142BBG70016);; 江西省自然科学基金(20142BAB214008; 20151BAB204003)共同资助
2020-08-25
2020-08-25