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己糖载体属于单糖转运蛋白家族,具有转运单糖类物质的作用,广泛存在于植物体各组织中并参与植物体的生长与发育。为了研究木薯己糖载体转运单糖的能力与对木薯生长发育的影响,本试验利用木薯全基因组数据库,通过RT-PCR方法从木薯KU50中克隆得到了木薯己糖载体基因MeSTP7,酵母异源表达与亚细胞定位实验结果表明MeSTP7具有转运葡萄糖、果糖、甘露糖和半乳糖的能力,定位在细胞膜上;qRT-PCR结果表明MeSTP7在高淀粉木薯品种KU50和低淀粉木薯品种CAS36根发育过程中的表达量均呈现逐渐降低的趋势,MeSTP7在KU50和CAS36的叶片中的表达量均为最高。本研究克隆得到了木薯己糖载体基因MeSTP7,并验证了MeSTP7的功能,为木薯己糖载体的研究提供了理论参考。
Abstract:Hexose transporters belong to the family of monosaccharide transpoters in plants, with the function of transporting monosaccharide. They widely distribute in plant tissues and affect the growth and development of plants. In order to study the ability of cassava hexose transporter in transporting monosaccharides and its effect on the growth and development of cassava, the full-length cDNA of a new hexose transporter gene MeSTP7 was cloned from cassva KU50 by RT-PCR. The heterologous expression of MeSTP7 in yeast demonstrated that MeSTP7 had the ability of transporting glucose, fructose, mannose and galactose. Subcellular localization indicated that MeSTP7 was a plasma membrane-localized hexose transporter. The results of qRT-PCR showed that the expression level of MeSTP7 decreased gradually during the rooting process in high-starch cassava cultivar KU50 and low-starch cassava cultivar CAS36, and the expression level of MeSTP7 in cassava leaves was the highest in the two kinds of cassavas. In this study, the cassava hexose transporter gene MeSTP7 was cloned and the function of MeSTP7 was verified, which provided a theoretical reference for the research of cassava hexose transporters.
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基本信息:
DOI:10.13417/j.gab.036.002032
中图分类号:Q943.2;S533
引用信息:
[1]武军政,刘秦,党会杰,等.木薯己糖载体基因MeSTP7的克隆与功能验证[J].基因组学与应用生物学,2017,36(05):2032-2039.DOI:10.13417/j.gab.036.002032.
基金信息:
国家自然科学基金(31660412)资助
2017-05-25
2017-05-25