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为有效防治百合枯萎病,对龙牙百合枯萎病菌进行鉴定、其生物学特性进行研究,并测定了10种杀菌剂对该病菌的室内毒力。经形态学和分子生物学鉴定,龙牙百合枯萎病菌为层出镰孢菌(Fusarium proliferatum)。该菌株菌丝生长的最适碳源为葡萄糖,氮源为牛肉浸膏;有较宽的酸碱度范围(p H4.0~10.0);菌丝生长的最适温度为25℃,致死温度为57℃,10 min;光周期12 L:12 D的环境条件利于菌体生长。采用菌丝生长速率法测定了10种杀菌剂对该菌株的室内毒力,数据显示,10种杀菌剂对层出镰孢菌菌丝的抑制效果有很大的差异。参试药剂中,12.5%烯唑醇WP,1%申嗪霉素SC的抑菌效果较好,EC50值分别为0.255 mg/L,0.731 mg/L;抑菌效果最差是25 g/L咯菌腈SC,EC50为89.294 mg/L。层出镰孢菌能够引发我国百合枯萎病,为国内首次报道。
Abstract：In order to effectively prevent and treat lily Fusarium wilt, this research identified the wilt pathogen of Lilium brownii var. viridulum and investigated its biological characteristics, as well as determined the indoor toxicity of 10 kinds of fungicides. The morphological and molecular identification showed that the wilt pathogen of Lilium brownii var. viridulum was identified as Fusarium proliferatum. The most suitable carbon source and nitrogen source for mycelium growth of F. proliferatum were glucose and beef extract, respectively, with a wide PH scale(p H 4.0~10.0); the optimum temperature for the mycelium growth was 25℃, while the lethal temperature was 57℃, 10 min; the suitable light condition for the mycelium growth was 12 L:12 D photoperiod. The indoor toxicity of 10 kinds of fungicides against F. proliferatum were measured by means of mycelium growth rate method. The results revealed that the inhibitory effects of 10 kinds of fungicides had great difference on the mycelium growth of F. proliferatum. Among the tested agents, 12.5% diniconazole WP and 1% shenqinmycin SC had better inhibitory effect. The EC50 values of these fungicides were 0.255 mg/L, 0.731 mg/L, respectively; the inhibitory effect of 25 g/L fludioxonil SC was the worst, with EC50 value of 89.294 mg/L. Fusarium proliferatum could cause Lilium brownii var. wilt in our country, which was reported for the first time in China.
An Z.H.,Huang D.Y.,Shi Y.X.,and Li B.J.,2010,Studies on fungicides controlling lily root Fusarium oxysporum fsplilii,Zhongguo Shucai(China Ve getables),1(18):23-26(安智慧,黄大野,石延霞,李宝聚,2010,百合镰刀菌枯萎病防治药剂的研究,中国蔬菜,1(18):23-26)
Chen K.S.,Li F.,Xu C.J.,Zhang S.L.,and Fu C.X.,2004,Trop.plant pathol,26(4):529-531(陈昆松,李方,徐昌杰,张上隆,傅承新,2004,改良CTAB法用于多年生植物组织基因组DNA的大量提取,遗传,26(4):529-531
Desjardins A.E.,Plattner R.D.,Nelson P.E.,1997,Production of fumonisin B1 and moniliformin by Gibberella fujikuroi from rice from various geographic areas,Appl.Environ.Microbiol,63:1838-1842
Du R.C.,and Wang J.S.,2013,Isolation,identification and laboratory toxicity determination of Fusarium oxysporum from watermelon root rot,Guangdong Nongye Daxue(Guangdong Agricultural Sciences),40(5):71-72(杜若琛,王金胜,2013,西瓜枯萎病菌分离、鉴定及室内毒力测定,广东农业科学,40(5):71-72)
Geng L.H.,Li C.B.,Chi S.Q.,Wang L.J.,and Chai M.,2012,Identification of the pathogen causing Fusarium crown and root rot of tomato and its growth affecting factors,Zhiwu Bingli Xuebao(Acta Phytopathologica Sinica),42(5):449-455(耿丽华,李长虎,迟胜起,王丽君,柴敏,2012,番茄颈腐根腐病病原鉴定及不同条件对其生长的影响,植物病理学报,42(5):449-455)
Leslie J.F.,Marasas W.F.O.,Shephard G.S.,Sydenham E.W.,Stockenstrêm S.,and Thiel P.G.,1996,Duckling toxicity and the production of fumonisin and moniliformin byisol ates in the A and F mating populations of Gibberella fujikuroi(Fusarium moniliforme),Appl.Environ.Microbiol,62(4):1182-1187
Li M.,Song Y.Z.,Yu Y.,Chen Z.M.,Liu G.K.,and Shen J.G.,2017,Identification of Fusarium proliferatum in introduced lily bulbs,Anhui Nongye Kexue(Journal of Anhui Agricultural Sciences),45(4):8-10(李敏,宋蕴哲,虞赟,陈智明,刘国坤,沈建国,2017,进境百合种球中镰刀菌的分离鉴定,安徽农业科学,45(4):8-10)
Li R G,Wang Y.,and Cheng H.,2016,Isolation and identification of the pathogen causing exsciccation of Lilium brownii var.viridulum and screening of control chemicals in laboratory,Huibei Nongye Kexue(Hubei Agricultural Sciences),55(10):2551-2554(李润根,王愉,程华,2016,百合枯萎病病原菌分离鉴定及防治药剂的室内筛选,湖北农业科学,55(10):2551-2554)
Li W.Y.,Liu Z.P.,He X.F.,and Wang W.H.,2016,The pathogen isolation,identification and pathogenicity of lily blight,Shenyang Nongye Daxue Xuebao(Journal of Shenyang A-gricultural University),47(2):153-158(李琬玥,刘正坪,何祥凤,王文和,2016,百合枯萎病病原菌的分离、鉴定及致病性研究,沈阳农业大学学报,47(2):153-158)
Liu C.,Xu W.,Liu F.,and Jiang S.,2007,Fumonisins production by Fusarium proliferatum strains isolated from asparagus crown,Mycopathologia,164(3):127-134
Liu J.P.,2014,Medicament efficacy test on Fusarium wilt disease of watermelon,Hebei Nongye Kexue(Journal of Hebei Agricultural Sciences),18(3):46-47(刘建平,2014,西瓜枯萎病药剂防治试验,河北农业科学,18(3):46-47)
Logrieco A.,Moretti A.,Ritieni A,and Corda P.,1995,Occurrence and toxigenicity of Fusaium proliferatum from preharvest maize ear rot,and associated mycotoxins,in Italy,Plant Disease,79(7):727-731
Luo D.W.,He S.,Guo L.J.,Cui J.,Luo Z.W.,Miao W.G.,Fan H.Y.,and He F.,2016,Identification and biological characteristics of the pathogens causing root rot disease of Artocarpus heteophyllus lam in Hainan province,Fenzi Zhiwu Yuzhong(Molecular Plant Breeding),14(8):2205-2212(罗敦文,何舒,郭利军,崔健,罗志文,缪卫国,范鸿雁,何凡,2016,海南省菠萝蜜根腐病病原菌鉴定及生物学特性分析,分子植物育种,14(8):2205-2212)
Prados-Ligero A.M.,Basallote-Ureba M.J.,and Melero-Vara J.M.,2008,First report of Fusarium oxysporum.f.sp.lilii and F.proliferatum affecting Lilium crops in Spain,Tropical Plant Pathology,33(3):235-236
Tang X.N.,You C.P.,Liu F.X.,Tang S.S.,Li Z.J.,Zhao Z.G.,and Zhang D.G.,1997,Survey and identification of Lilium spp.disease in Jiangxi China,Jiangxi Nongye Xuebao(Acta Agriculture Jiangxi),9(4):1-8(唐祥宁,游春平,刘福秀,汤水生,李志坚,赵招姑,张德贵,1997,江西百合病害调查与鉴定,江西农业学报,9(4):1-8)
Wu Y.G.,and Jiang L.L.,2015,Study on indoor toxicity of 8fungicides to cucumber Fusarium wilt,Pesticide Nongyao Kexue Guanli(Pesticide Science and Administration),36(4):54-56,62(武玉国,姜莉莉,2015,8种杀菌剂对黄瓜枯萎病菌的室内毒力测定,农药科学与管理,36(4):54-56,62)
Xu J.,Hu L.,Jiang Y.,and Xu X.D.,2013,Comparison of the biological characteristics of Fusarium subglutinans and Fusarium proliferatum,Liaoning Nongye Kexue(Liaoning Agricultural Sciences),(1):1-5(徐婧,胡兰,姜钰,徐秀德,2013,玉米顶腐病和鞘腐病致病镰孢菌生物学特性比较,辽宁农业科学,(1):1-5)
Xu X.Y.,Jiang Y.,Wang L.J.,Dong H.Y.,Hu L.,Lv G.Z.,and Liu Z.H.,2008,Corn sheath rot-a new disease found in China,Zhongguo Nongye Kexue(Scientia Agricultura Sinica),(10):3083-3087(徐秀德,姜钰,王丽娟,董怀玉,胡兰,吕国忠,刘志恒,2008,玉米新病害---鞘腐病研究初报,中国农业科学,(10):3083-3087)
Yang S.,Wang J.S.,Ma Z.C.,Wang Y.C.,and Wang K.R.,2012,Isolation and identification of Fusarium proliferatum causing soybean root rot and its biological characterization,Zhiwu Baohu Xuebao(Acta Phytophylacica Sinica),39(2):187-188(杨帅,王健生,马振川,王源超,王克荣,2012,引致大豆根腐病的层出镰孢菌分离鉴定及其特性,植物保护学报,39(2):187-188)
Zhang C.C.,Wang H.,He C.P.,Liang Y.Q.,Zheng J.L.,Xi J.G.,Zheng X.L.,Tang W.,Xu P.D.,Li R.,Wu W.H.,and Yi K.X.,2016,Identification and biological characteristics analysis on the pathogen of Pennisetum purpureumcv Guimu-1leaf spot disease,Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),35(5):1232-1239(张驰成,汪涵,贺春萍,梁艳琼,郑金龙,习金根,郑肖兰,唐文,许沛东,李锐,吴伟怀,易克贤,2016,桂牧1号杂交象草叶斑病病原鉴定及其生物学特性分析,基因组学与应用生物学,35(5):1232-1239)
Zhang J.G.,Qi Y.H.,Guo C.,Su J.H.,Li M.Q.,Li X.P.,Cao S.F.,and Liu D,2016,Identification of pathogens causing shallot storage Fusarium rot disease in Gansu province,China,Weishengwuxue Tongbao(Microbiology),43(10):2216-2224(张军高,漆永红,郭成,苏建红,李敏权,李雪萍,曹素芳,刘丹,2016,甘肃大葱贮藏期镰孢菌腐烂病病原鉴定,微生物学通报,43(10):2216-2224)
Zhu H.Y.,Xia H.,and Gao B.D.,2012,Biological characteristics of pathogen of edible lily bulb rot and fungicide screening for interior control in Longhui county,Hunan Nongye Kexue(Hunan Agricultural Sciences),(13):81-84(朱海燕,夏花,高必达,2012,隆回县食用百合鳞茎腐烂病菌生物学特性及室内防治药剂筛选,湖南农业科学,(13):81-84)
Zhu M.S.,Guan T.S.,and Lu Z.H.,2010,Toxicity of Thirteen Fungicides to Fusarium oxysporum f.sp lili Snyder et Hasen,Nongyao(Agrochemicals),49(10):760-761(朱茂山,关天舒,吕振环,2010,13种杀菌剂对百合枯萎病菌的室内毒力测定,农药,49(10):760-761)
基本信息:
DOI：10.13417/j.gab.037.003844
中图分类号:S436.8
引用信息:
[1]李润根,程华,张鹏,等.百合枯萎病菌Fusarium proliferatum的鉴定、生物学特性及抑菌药剂筛选[J].基因组学与应用生物学,2018,37(09):3844-3851.DOI:10.13417/j.gab.037.003844.
基金信息:
江西省科技厅重点研发项目(20151BBF60050;20161BBF60040)资助
2018-09-20
2018-09-20