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钙依赖性蛋白激酶(calcium-dependent protein kinases,CDPKs or CPKs)作为一类钙感知蛋白在植物的生长发育和胁迫应答中起着重要的作用。LeCPK2(GenBank accession No.:GQ205414)是我们从番茄中分离的第3个CDPK基因,前期研究表明LeCPK2可能在植物热胁迫应答中发挥作用。为了进一步研究其在热胁迫中的功能,我们通过电子克隆的方法分离了LeCPK2的启动子序列,并通过LeCPK2过表达烟草分析其在高温胁迫中的潜在的功能。生物信息学分析显示,LeCPK2启动子中包含5个热响应元件,和前期试验结果一致。野生型植株在受到热胁迫后,对光更为敏感,强光照下植株叶片发生萎蔫,而强光本身不会对未受热胁迫的健康植株造成伤害。LeCPK2转基因植株热、光胁迫后不会出现受害表型。以上研究表明,LeCPK2在植物的热胁迫应答中发挥重要作用,能够有效保护植株免受高温胁迫的损害,是一个优秀的耐热(光)基因。本研究将为揭示番茄LeCPK2遗传功能及对其开发利用奠定基础。
Abstract：Calcium-dependent protein kinases (CDPKs) may function as calcium sensors and play important roles in the regulation of plant growth and development and in plant responses to biotic and abiotic stresses.LeCPK2 (GenBank accession No.:GQ205414) is the third CDPK gene isolated from tomato and is observed to play roles in the response to heat stress in our previous studies.To further characterize the role of LeCPK2 in heat stress,we isolated the LeCPK2 promoter by in silico cloning and investigated the physiological roles of LeCPK2 under heat stress using LeCPK2 overexpression tobacco plants.Bioinformatic analysis showed that the promoter region of LeCPK2 contained five cis-regulatory elements responsive to heat stress,which was in accord with our previous experimental results.The wild-type tobacco plants stressed by high temperature were observed to be sensitive to light and thus exhibited obvious wilt of leaves.However,the strong light would not harm the healthy wild-type plants untreated by heat.Contrarily,the LeCPK2 overexpression plants would not be harmed by high temperature and following strong light stress.All the results suggested that the tomato LeCPK2 may be an excellent heat (light)-tolerant gene,which was involved in the response to heat stress and can effectively protect plants from the harms of heat (light) stress.Our studies will lay a foundation for the discovery of the genetic roles and for the development and application of LeCPK2.
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基本信息:
中图分类号:S641.2
引用信息:
[1]畅文军,付桂,陈鑫,等.番茄钙依赖性蛋白激酶基因LeCPK2在热(光)胁迫中的功能鉴定[J].基因组学与应用生物学,2011,30(04):338-345.
基金信息:
国家自然科学基金(30760917);; 中央级公益性科研院所基本科研业务费专项(ITBB0936)
2011-08-28
2011-08-28