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本研究以‘大五星’(红肉)和‘川农1-5-9’(白肉)枇杷不同发育阶段的果皮、果肉为材料,采用同源克隆法从枇杷果实中克隆类胡萝卜素合成途径中关键酶基因PSY,利用生物信息学方法分析所获得的核苷酸序列及推导的氨基酸序列,再利用qRT-PCR从转录水平上检测两种枇杷不同发育阶段表达水平。发现枇杷PSY基因cDNA序列全长1 191 bp,具有完整开放阅读框,编码396个氨基酸。氨基酸同源性分析表明,枇杷PSY蛋白与其他物种PSY蛋白具有很高的相似性,与苹果(Malus domestica)相似性高达98%。qRT-PCR分析表明,随着枇杷果实的发育,PSY基因表达量明显增加,与转色期后枇杷总类胡萝卜素含量变化一致,表明PSY基因可能参与枇杷类胡萝卜素合成的调控。
Abstract：In the study, the different developmental stages of loquat peel and pulp(Eriobotrya japonica Lindl.'Dawuxing' and 'Chuannong 1-5-9') were selected. The PSY gene was cloned from loquat fruit by using RT-PCR,bioinformatics methods were used to analyze c DNA sequence obtained and putative amino acid sequence,Real-time PCR were used to study the expression of PSY gene in different developmental stages. The full-length sequence consisted of 1 191 bp with an intact open reading frame and encoding 396 amino acids. Homology analysis indicated that the deduced PSY protein was highly homologous to other PSY proteins, and had a similarity of 98%with apple(Malus domestica). Analysis of Real-time PCR showed that the expression of PSY gene increased significantly, which was consistent with the accumulation of carotenoid after loquat colour-changed period. The result showed that PSY gene may participate in carotene biosynthesis.
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基本信息:
DOI：10.13417/j.gab.037.004407
中图分类号:S667.3;Q943.2
引用信息:
[1]洪敏,池卓恒,石丝,等.枇杷八氢番茄红素合成酶基因的克隆及表达分析[J].基因组学与应用生物学,2018,37(10):4407-4413.DOI:10.13417/j.gab.037.004407.
基金信息:
四川省科技支撑计划项目(2016NYZ0034)资助
2017-08-08
2017-08-08
2017-08-08