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本研究采集三峡水库库区6目10科41属51种常见鱼类样本,测定了DNA条形码COI基因652 bp同源片段,初步分析条形码序列特征、不同分类水平下K2P遗传距离及系统发育关系。研究发现:属内种间平均遗传距离为0.061,科内属间平均遗传距离为0.110,目内科间平均遗传距离为0.206。DNA条形码具有较高的物种鉴别率,49种(占总种类的96.1%)鱼类可形成明显的条形码间隙。NJ树拓扑结构也显示种、属水平的单系性得到较好支持,部分科及以上水平系统关系与形态分类结果略有差异。利用分子生物学数据库工具,以202条鱼类线粒体COI序列构建了小型DNA条形码数据库,序列检索结果显示:待检样品种类鉴定正确,与形态学鉴定结果一致。该数据库可实现该区域鱼类的快速、准确鉴定,以期为该地区鱼类生物多样性的保护和种群动态监测提供技术支持。
Abstract：In this study, 51 species of common fish collected from the Three Gorges Reservoir were barcoded for a652 bp region of the mitochondrial cytochrome oxidase subunit I(COI) gene. These common fish belonged to 41 genera, 10 families, and 6 orders. The results showed that the average Kimura's 2±parameter(K2 P) distance within the genus, within the family and order were 0.061, 0.110, and 0.206, respectively. The barcode gaps were found in 49 of 51 species analysed with multiple specimens(96.1%), with the minimum interspecific distance to the nearest neighbor larger than the maximum intraspecific distance. Althogh, most sequences of the same species were clustered together in the NJ tree, senior taxa were different from the morphological conclusion. To construct a local DNA barcoding library to make species identification more effective, 51 DNA barcode sequences were constructed by using database tools provided by the National Center for Biotechnology Information(NCBI). Local BLAST database of DNA barcode was constructed successfully, the species could be confirmed by the database. The DNA barcode library established in this study will shed light on further research on the molecular identification in this region.
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基本信息:
DOI：10.13417/j.gab.040.001952
中图分类号:S917.4
引用信息:
[1]郜星晨,姜伟.三峡库区常见鱼类DNA条形码本地BLAST数据库的构建和应用[J].基因组学与应用生物学,2021,40(Z1):1952-1960.DOI:10.13417/j.gab.040.001952.
基金信息:
国家重点研发计划重点专项(2016YFC0502210); 农业农村部长江流域渔政监督管理办公室专项(CYB-20191123)共同资助
2020-11-06
2020-11-06
2020-11-06