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血栓是威胁现代人体健康的重要因素之一，高活力的纤维蛋白溶解酶对于血栓的溶栓治疗具有非常重要的意义。本研究以转录组分析和酶动力学为基础，主要探究了三价铁离子胁迫对海洋源解淀粉芽孢杆菌(Bacillus amyloliquefaciens) GXU-1产纤维蛋白溶解酶的调控机制。经过RNA测序（RNA sequencing，RNA-seq）及实时定量PCR（real-time quantitative PCR，RT-qPCR）验证显示，0.05 g/L氯化铁胁迫使酶活提高1.47倍。转录组分析鉴定到1 607个显著差异表达基因，其中上调970个，下调637个。与酶表达密切相关的22个基因表达上调，主要富集于氧化磷酸化、生物膜代谢及丝氨酸蛋白酶合成等通路。关键上调基因包括bpr(5.31倍)、isp(4.85倍) 和aprE(4.40倍)，另外与生物膜相关基因如dacB(5.60倍) 和dacF(4.97倍) 上调则促进了酶的分泌。RT-qPCR验证了15个代表性基因的一致表达趋势。最终发现三价铁离子主要是通过激活氧化磷酸化及生物膜相关通路，为酶合成提供能量和前体，从而起到增强纤维蛋白溶解酶活性的作用。这些发现为菌株的后期工程改造提供了分子靶点，同时丰富了枯草芽孢杆菌组的基因组学资源。
Abstract：Thromboembolism poses a serious threat to human health， and high-efficiency fibrinolytic enzymes are crucial for thrombolytic therapy. Based on transcriptomic analysis and enzyme kinetics， this study investigated the regulatory mechanism of ferric ion (Fe3?) stress on fibrinolytic enzyme production in the marine-derived bacterium Bacillus amyloliquefaciens GXU-1. RNA sequencing（RNA-seq）and real-time quantitative PCR（RT-qPCR）validation showed that 0.05 g/L ferric chloride stress increased enzyme activity by 1.47-fold. Transcriptomic analysis identified 1 607 significantly differentially expressed genes， of which 970 were upregulated and 637 were downregulated. Twenty-two genes closely associated with enzyme expression were upregulated， enriched in pathways such as oxidative phosphorylation， biofilm metabolism， and serine protease synthesis. Key upregulated genes included bpr (5.31-fold)， isp (4.85-fold)， and aprE (4.40-fold)， while biofilm-related genes such as dacB (5.60-fold) and dacF (4.97-fold) promoted enzyme secretion. RT-qPCR verified the consistent expression trends of 15 representative genes. Ferric ions primarily enhance fibrinolytic enzyme activity by activating oxidative phosphorylation and biofilm-related pathways， thereby providing energy and precursors for enzyme synthesis. These findings provide molecular targets for strain engineering and enrich the omics resources of the Bacillus subtilis group.
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基本信息:
中图分类号:Q78
引用信息:
[1]赵丁,龚绵红,史芮源,等.基于基因组与转录组分析揭示解淀粉芽孢杆菌GXU-1响应铁胁迫高效合成纤维蛋白溶解酶的机制[J].基因组学与应用生物学().
基金信息:
广西重点研发项目（2025FN96441173）资助
2026-05-20
2026-05-20
2026-05-20