| 1,183 | 2 | 133 |
| 下载次数 | 被引频次 | 阅读次数 |
为探究番茄(Solanum lycopersicum)灰霉病的致病机理与β-氨基丁酸(β-aminobutyric acid, BABA)提高番茄灰霉病耐受性的作用机制，本文通过GEO数据库检索获取与番茄灰霉病相关的基因芯片数据集，利用STEM软件和Limma软件包，以及构建加权基因共表达网络(weighted gene co-expression network analysis, WGCNA)进行联合分析。实验结果表明，番茄灰霉病的发生抑制了光合作用、糖代谢与能量代谢、次生代谢物的合成，同时激活植物激素信号转导途径、植物MAPK信号通路、核糖体生物发生、氨基酸的生物合成、谷胱甘肽代谢等防御反应；而BABA处理后诱导生长素应答因子、赤霉素受体、谷胱甘肽酰氢醌还原酶、交替氧化酶、异黄酮代谢相关酶、 bZIP转录因子等28个关键抗性基因的表达，通过更为复杂的植物激素信号转导途径、植物MAPK信号通路、细胞壁生物合成、精氨酸和脯氨酸合成、次生代谢物生物合成等途径，提高了番茄灰霉病抗性。本研究从分子生物学水平阐明了番茄灰霉病的致病机理以及BABA提高番茄灰霉病抗性的作用机制，为培育番茄灰霉病抗性品种提供了研究依据。
Abstract：In order to explore the pathogenesis of tomato(Solanum lycopersicum) gray mold and the mechanism of β-aminobutyric acid(BABA)to improve the tolerance of tomato gray mold. In this study, gene chip data set related to tomato gray mold were retrieved from GEO database, and analyzed jointly using STEM software, Limma software package and construction of weighted gene co-expression network analysis(WGCNA). The results showed that the occurrence of tomato boreal mildew inhibited photosynthesis, glucose and energy metabolism, and the synthesis of secondary metabolites, and activated the plant hormone signal transduction pathway, plant MAPK signal pathway, ribosome biogenesis, amino acid biosynthesis, glutathione metabolism and other defense reactions. While BABA treatment induced the expression of 28 key resistance genes, including auxin response factor, gibberellin receptor, glutathione hydroquinone reductase, alternate oxidase, isoflavone metabolism related enzyme, and bZIP transcription factor, etc. And more complex plant hormone signal transduction pathway, plant MAPK signal pathway, cell wall biosynthesis, arginine and proline synthesis, secondary metabolite biosynthesis and other pathways increased the resistance of tomato gray mold. This study elucidated the pathogenic mechanism of tomato gray mold and the mechanism of BABA to improve the resistance of tomato gray mold from the molecular biological level, providing a research basis for cultivating tomato gray mold resistant varieties.
ABUQAMAR S,MOUSTAFA K,TRAN L S,2017.Mechanisms and strategies of plant defense against Botrytis cinerea.Crit.Rev.Biotechnol.,37(2):262-274.
ADACHI H,ISHIHAMA N,NAKANO T,et al.,2016.Nicotiana benthamiana MAPK-WRKY pathway confers resistance to a necrotrophic pathogen Botrytis cinerea.Plant Signal.Behav.,11(6):e1183085.
ADAMIPOUR N,KHOSH-KHUI M,SALEHI H,et al.,2020.Metabolic and genes expression analyses involved in proline metabolism of two rose species under drought stress.Plant Physiol.Biochem.,155:105-113.
BACETE L,MéLIDA H,MIEDES E,et al.,2018.Plant cell wall-mediated immunity:cell wall changes trigger disease resistance responses.Plant J.,93(4):614-636.
BAUTERS L,STOJILKOVI■.Pathogens pulling the strings:effectors manipulating salicylic acid and phenylpropanoid biosynthesis in plants.Mol.Plant Pathol.,22(11):1436-1448.
BI K,SCALSCHI L,JAISWAL N,et al.,2021.The Botrytis cinerea Crh1 transglycosylase is a cytoplasmic effector triggering plant cell death and defense response.Nat.Commun.,12:2166.
BJORNSON M,ZIPFEL C,2021.Plant immunity:Crosstalk between plant immune receptors.Curr.Biol.,31(12):R796-R798.
CATONI M,ALVAREZ-VENEGAS R,WORRALL D,et al.,2022.Long-lasting defence priming by β-aminobutyric acid in tomato is marked by genome-wide changes in DNA methylation.Front.Plant Sci.,13:836326.
CEULEMANS E,IBRAHIM H M M,DE CONINCK B,et al.,2021.Pathogen effectors:exploiting the promiscuity of plant signaling hubs.Trends Plant Sci.,26(8):780-795.
COHEN Y,NIDERMAN T,MOSINGER E,et al.,1994.β-aminobutyric acid induces the accumulation of pathogenesis-related proteins in tomato (Lycopersicon esculentum L.) plants and resistance to late blight infection caused by Phytophthora infestans.Plant Physiol.,104(1):59-66.
CUI K D,HE L M,LI T T,et al.,2021.Development of boscalid resistance in Botrytis cinerea and an efficient strategy for resistance management.Plant Dis.,105(4):1042-1047.
CVIKROVá M,GEMPERLOVá L,MARTINCOVá O,et al.,2013.Effect of drought and combined drought and heat stress on polyamine metabolism in proline-over-producing tobacco plants.Plant Physiol.Biochem.,73:7-15.
DEAN R,VAN KAN J A L,PRETORIUS Z A,et al.,2012.The top 10 fungal pathogens in molecular plant pathology.Mol.Plant Pathol.,13(4):414-430.
DELPLACE F,HUARD-CHAUVEAU C,BERTHOMé R,et al.,2022.Network organization of the plant immune system:from pathogen perception to robust defense induction.Plant J.,109(2):447-470.
DENNIS G,SHERMAN B T,HOSACK D A,et al.,2003.DAVID:Database for annotation,visualization,and integrated discovery.Genome Biol.,4(5):P3.
DODDS P N,RATHJEN J P,2010.Plant immunity:towards an integrated view of plant-pathogen interactions.Nat.Rev.Genet.,11(8):539-548.
DOROODIAN P,HUA Z H,2021.The ubiquitin switch in plant stress response.Plants,10(2):246.
ELSHERBINY E A,DAWOOD D H,SAFWAT N A,2021.Antifungal action and induction of resistance by β-aminobutyric acid against Penicillium digitatum to control green mold in orange fruit.Pestic.Biochem.Physiol.,171:104721.
ERNST J,BAR-JOSEPH Z,2006.STEM:a tool for the analysis of short time series gene expression data.BMC Bioinform.,7:191.
ERNST J,NAU G J,BAR-JOSEPH Z,2005.Clustering short time series gene expression data.Bioinformatics,21(suppl_1):i159-i168.
FERRARI S,GALLETTI R,DENOUX C,et al.,2007.Resistance to Botrytis cinerea induced in Arabidopsis by elicitors is independent of salicylic acid,ethylene,or jasmonate signaling but requires PHYTOALEXIN DEFICIENT3.Plant Physiol.,144(1):367-379.
GAO M M,ZHANG N,LIANG W X,2020.Systematic analysis of lysine lactylation in the plant fungal pathogen Botrytis cinerea.Front.Microbiol.,11:594743.
GAZARA R K,MOHARANA K C,BELLIENY-RABELO D,et al.,2018.Expansion and diversification of the gibberellin receptor GIBBERELLIN INSENSITIVE DWARF1 (GID1) family in land plants.Plant Mol.Biol.,97(4-5):435-449.
GUR L,REUVENI M,COHEN Y,2021.β-aminobutyric acid induced resistance against Alternaria fruit rot in apple fruits.J.Fungi,7(7):564.
JIA H R,LI T,HAIDER M S,et al.,2022.Comparative transcriptomic and metabolomic profiling of grapevine leaves (cv.kyoho) upon infestation of grasshopper and Botrytis cinerea.Plant Mol.Biol.Report.,40(3):539-555.
KIM Y K,XIAO C L,2010.Resistance to pyraclostrobin and boscalid in populations of Botrytis cinerea from stored apples in Washington state.Plant Dis.,94(5):604-612.
KOZHAR O,PEEVER T L,2018.How does Botrytis cinerea infect red raspberry?Phytopathology,108(11):1287-1298.
LANGFELDER P,HORVATH S,2008.WGCNA:an R package for weighted correlation network analysis.BMC Bioinform.,9:559.
LI R,SHENG J P,SHEN L,2020.Nitric oxide plays an important role in β-aminobutyric acid-induced resistance to Botrytis cinerea in tomato plants.Plant Pathol.J.,36(2):121-132.
LI T T,FAN P H,YUN Z,et al.,2019.β-aminobutyric acid priming acquisition and defense response of mango fruit to Colletotrichum gloeosporioides infection based on quantitative proteomics.Cells,8(9):1029.
LIU H L,LI Y G,HU Y L,et al.,2021.EDS1-interacting J protein 1 is an essential negative regulator of plant innate immunity in Arabidopsis.Plant Cell,33(1):153-171.
LIU M Y,ZHANG Q X,WANG C,et al.,2020.CsWRKY10 mediates defence responses to Botrytis cinerea infection in Cucumis sativus.Plant Sci.,300:110640.
MA X H,XU J Y,HAN D,et al.,2020.Combination of β-aminobutyric acid and Ca2+ alleviates chilling stress in tobacco (Nicotiana tabacum L.).Front.Plant Sci.,11:556.
MAHMUD J A,HASANUZZAMAN M,KHAN M I R,et al.,2020.β-aminobutyric acid pretreatment confers salt stress tolerance in Brassica napus L.by modulating reactive oxygen species metabolism and methylglyoxal detoxification.Plants.9(2):241.
MáTAI A,JAKAB G,HIDEG é,2019.Single-dose β-aminobutyric acid treatment modifies tobacco (Nicotiana tabacum L.) leaf acclimation to consecutive UV-B treatment.Photochem.Photobiol.Sci.,18(2):359-366.
NAKAJIMA M,AKUTSU K,2014.Virulence factors of Botrytis cinerea.J.Gen.Plant Pathol.,80(1):15-23.
NGOU B P M,JONES J D G,DING P T,2022.Plant immune networks.Trends Plant Sci.,27(3):255-273.
NOMAN A,LIU Z Q,AQEEL M,et al.,2017.Basic leucine zipper domain transcription factors:the vanguards in plant immunity.Biotechnol.Lett.,39(12):1779-1791.
PER T S,KHAN N A,REDDY P S,et al.,2017.Approaches in modulating proline metabolism in plants for salt and drought stress tolerance:phytohormones,mineral nutrients and transgenics.Plant Physiol.Biochem.,115:126-140.
PETRASCH S,KNAPP S J,VAN KAN J A L,et al.,2019.Grey mould of strawberry,a devastating disease caused by the ubiquitous necrotrophic fungal pathogen Botrytis cinerea.Mol.Plant Pathol.,20(6):877-892.
REN X Y,WANG J J,ZHU F L,et al.,2022.β-aminobutyric acid (BABA)-induced resistance to tobacco black shank in tobacco (Nicotiana tabacum L.).PLoS ONE,17(6):e0267960.
RITCHIE M E,PHIPSON B,WU D,et al.,2015.Limma powers differential expression analyses for RNA-sequencing and microarray studies.Nucleic Acids Res.,43(7):e47.
ROBIN A H K,SAHA G,LAILA R,et al.,2020.Expression and role of biosynthetic,transporter,receptor,and responsive genes for auxin signaling during clubroot disease development.Int.J.Mol.Sci.,21(15):5554.
RUSSELL T M,RICHARDSON D R,2022.Glutathione-S-transferases as potential targets for modulation of nitric oxide-mediated vasodilation.Biomolecules,12(9):1292.
SALEHIN M,BAGCHI R,ESTELLE M,2015.SCFTIR1/AFB-based auxin perception:mechanism and role in plant growth and development.Plant Cell,27(1):9-19.
SHANNON P,MARKIEL A,OZIER O,et al.,2003.Cytoscape:a software environment for integrated models of biomolecular interaction networks.Genome Res.,13(11):2498-2504.
SHAO W Y,ZHAO Y F,MA Z H,2021.Advances in understanding fungicide resistance in Botrytis cinerea in China.Phytopathology,111(3):455-463.
SHAW A K,BHARDWAJ P K,GHOSH S,et al.,2016.β-aminobutyric acid mediated drought stress alleviation in maize (Zea mays L.).Environ.Sci.Pollut.Res.,23(3):2437-2453.
SHEN W T,SONG Z G,ZHONG X A,et al.,2022.Sangerbox:a comprehensive,interaction-friendly clinical bioinformatics analysis platform.iMeta,1(3):e36.
SILVA C J,ADASKAVEG J A,MESQUIDA-PESCI S D,et al.,2023.Botrytis cinerea infection accelerates ripening and cell wall disassembly to promote disease in tomato fruit.Plant Physiol,191(1):575-590.
SIVAKUMARAN A,AKINYEMI A,MANDON J,et al.,2016.ABA suppresses Botrytis cinerea elicited NO production in tomato to influence H2O2 generation and increase host susceptibility.Front.Plant Sci.,7:709.
SOUSA B,LOPES J,LEAL A,et al.,2021.Specific glutathione-S-transferases ensure an efficient detoxification of diclofenac in Solanum lycopersicum L.plants.Plant Physiol.Biochem.,168:263-271.
SUKUMARAN A,MCDOWELL T,CHEN L,et al.,2018.Isoflavonoid-specific prenyltransferase gene family in soybean:GmPT01,a pterocarpan 2-dimethylallyltransferase involved in glyceollin biosynthesis.Plant J.,96(5):966-981.
SULEMAN M,MA M,GE G,et al.,2021.The role of alternative oxidase in plant hypersensitive response.Plant Biol.,23(3):415-419.
TANG J J,WU M D,ZHANG J,et al.,2021.Botrytis cinerea G protein β subunit Bcgb1 controls growth,development and virulence by regulating cAMP signaling and MAPK signaling.J.Fungi,7(6):431.
THEVENET D,PASTOR V,BACCELLI I,et al.,2017.The priming molecule β-aminobutyric acid is naturally present in plants and is induced by stress.N.Phytol.,213(2):552-559.
TORRES-GUZMAN J C,PADILLA-GUERRERO I E,CERVANTES-QUINTERO K Y,et al.,2021.Peculiarities of nitronate monooxygenases and perspectives for in vivo and in vitro applications.Appl.Microbiol.Biotechnol.,105(21-22):8019-8032.
VAN DE POEL B,SMET D,VAN DER STRAETEN D,2015.Ethylene and hormonal cross talk in vegetative growth and development.Plant Physiol.,169(1):61-72.
WAN R,GUO C L,HOU X Q,et al.,2021.Comparative transcriptomic analysis highlights contrasting levels of resistance of Vitis vinifera and Vitis amurensis to Botrytis cinerea.Hortic.Res.,8:103.
WANG J,CAO S F,WANG L,et al.,2018.Effect of β-aminobutyric acid on disease resistance against Rhizopus rot in harvested peaches.Front.Microbiol.,9:1505.
XIONG W,LAN T,MO B X,2021.Extraribosomal functions of cytosolic ribosomal proteins in plants.Front.Plant Sci.,12:607157.
XU D D,YU G,XI P G,et al.,2018.Synergistic effects of resveratrol and pyrimethanil against Botrytis cinerea on grape.Molecules,23(6):1455.
YAN L,LI S,RIAZ M,et al.,2021.Proline metabolism and bio-synthesis behave differently in response to boron-deficiency and toxicity in Brassica napus.Plant Physiol.Biochem.,167:529-540.
YANG Y K,YANG X F,DONG Y J,et al.,2018.The Botrytis cinerea xylanase BcXyl1 modulates plant immunity.Front.Microbiol.,9:2535.
YIN X J,LIU S,QIN Y K,et al.,2021.Metabonomics analysis of drought resistance of wheat seedlings induced by β-aminobutyric acid-modified chitooligosaccharide derivative.Carbohydr.Polym.,272:118437.
YUAN M H,JIANG Z Y,BI G Z,et al.,2021.Pattern-recognition receptors are required for NLR-mediated plant immunity.Nature,592(7852):105-109.
ZHANG H,ZHANG X,LIU J A,et al.,2020.Characterization of the heavy-metal-associated isoprenylated plant protein (HIPP) gene family from triticeae species.Int.J.Mol.Sci.,21(17):6191.
ZHANG P A,JIA H F,GONG P J,et al.,2021.Chitosan induces jasmonic acid production leading to resistance of ri-pened fruit against Botrytis cinerea infection.Food Chem.,337:127772.
ZHAO Y,ZHU X B,CHEN X W,et al.,2022.From plant immunity to crop disease resistance.J.Genet.Genom.,49(8):693-703.
基本信息:
DOI：10.13417/j.gab.042.001058
中图分类号:S436.412.13
引用信息:
[1]杨巍,赵丽芬,唐兵,等.番茄灰霉病关键抗性基因与信号通路的生物信息学分析[J].基因组学与应用生物学,2023,42(10):1058-1078.DOI:10.13417/j.gab.042.001058.
基金信息:
贵州省科技计划项目(黔科合服企[2022]005); 贵州省基层农技推广体系改革与建设补助项目(GZNJTG-WN-2023-01); 贵阳市蔬菜种质资源研究中心建设项目(筑科合同[2021]5-1号); 省科技计划项目(黔科合支撑[2020]1Y090号)共同资助
2023-06-08
2023-06-08
2023-06-08