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活性氧自由基(ROS)是细胞有氧代谢过程中产生的一类活性物,利用食源蛋白制备具有抗氧化能力的天然活性肽是当前的研究热点。本研究以机械冷榨芝麻粕为原料,比较了解淀粉芽孢杆菌、植物乳杆菌单菌及其混菌发酵芝麻粕制备芝麻多肽的过程,以DPPH·与·OH清除能力为评价指标,研究其体外抗氧化活性。数据表明,解淀粉芽孢杆菌、植物乳杆菌单菌及其混菌发酵芝麻粕36 h,芝麻多肽提取液中芝麻多肽的浓度分别达到9.841 mg/m L、11.620 mg/m L、14.412 mg/m L,DPPH·清除率分别是71.378%、77.013%、87.622%,·OH清除率分别是68.427%、82.507%、92.460%。因此,芝麻多肽具有较强的抗氧化活性,芝麻粕是制备抗氧化活性肽的良好原料;微生物发酵制备芝麻多肽时,解淀粉芽孢杆菌与植物乳杆菌混菌发酵显著优于单菌发酵,其多肽产量及DPPH·与·OH清除能力均优于相应单菌发酵。
Abstract：Reactive Oxygen Species(ROS) produced in the process of cell aerobic metabolism is a kind of active species.The preparation of natural active peptides with antioxidant ability from food protein is a researching hotspot currently.In this research,the mechanically cold pressed sesame meal was used as raw material.We compared the preparation of sesame polypeptide from fermentation of sesame meal with Bacillus amyloliquefaciens,Lactobacillus plantarum subsp.plantarum and the mixture of above.DPPH · and ·OH scavenging ability was taken as evaluation index to study the antioxidant activity in vitro.The results showed that,after the fermentation of sesame meal with Bacillus amyloliquefaciens,Lactobacillus plantarum subsp.Plantarum and the mixed bacteria for 36 h,the concentrations of sesame polypeptide in the extracts were 9.841 mg/m L,11.620 mg/m L,14.412 mg/m L,respectively,the DPPH· scavenging ability were 71.378%,77.013%,87.622%,and the ·OH scavenging ability were 68.427%,82.507%,92.460%,accordingly.Therefore,sesame polypeptide showed high antioxidant activity,and sesame meal was a good raw material for the preparation of antioxidant active peptides.During the the preparation of sesame polypeptide by the fermentation of microorganism,there were more sesame polypeptides with the higher DPPH·and·OH scavenging ability could be extracted from the mixed fermentation of Bacillus amyloliquefaciens and Lactobacillus plantarum subsp.Plantarum than the corresponding single strain.
Anastasiou D.,Poulogiannis G.,Asara J.M.,Boxer M.B.,Jiang J.K.,Shen M.,Bellinger G.,Sasaki A.T.,Locasale J.W.,and Auld D.S.,2011,Inhibition of pyruvate kinase M2 by reactive oxygen species contributes to cellular antioxidant responses,Science,334(6060):1278-1283
Chen X.H.,Qin W.D.,Ma L.H.,and Wang Y.B.,2010,Study on the production of sesame protein peptides with microorganism fermentation,Shipin Gongye Keji(Science and Technology of Food Industry),31(6):237-238(陈学红,秦卫东,马利华,王一彪,2010,微生物发酵法制备芝麻蛋白肽的研究,食品工业科技,31(6):237-238)
Clemente A.,2000,Enzymatic protein hydrolysates in human nutrition,Trends in Food Science Technology,11(7):254-262
Cotton F.,Delobbe E.,and Gulbis B.,1997,Assessment of a biuret method without concentration step for total protein determination in cerebrospinal fluid,Clinical Biochemistry,30(4):313-314
Frans en M.,Nordgren M.,Wang B.,and Apanasets O.,2012,Role of peroxisomes in ROS/RNS-me tabolism:implications for human disease,Biochimica et Biophysica Acta(BBA)-Molecular Basis of Disease,1822(9):1363-1373
Hu Y.N.,Li A.K.,Wang Z.S.,Zhang X.L.,Liu G.M.,and Han W.,2012,Study on the nutritional character of rapeseed meal by microbial solid state fermentation,Zhongguo Liangyou Xuebao(Journal of the Chinese Cereals and Oils Association),27(3):76-80(胡永娜,李爱科,王之盛,张晓琳,刘国明,韩伟,2012,微生物固态发酵菜籽粕营养特性的研究,中国粮油学报,27(3):76-80)
Jung W.K.,Rajapakse N.,and Kim S.K.,2005,Antioxidative activity of a low molecular weight peptide derived from the sauce of fermented blue mussel,Mytilus edulis,European Food Research and Technology,220(5):535-539
Lan S.L.,Mao X.W.,Xiao T.Y.,Wang H.Q.,Deng Y.Y.,and Tan B.,2013,Detoxification conditions of solid-state fermentation from rapeseed meal by mixture strains using response surface methodology,Dongwu Yingyang Xuebao(Chinese Journal of Animal Nutrition),25(3):617-627(兰时乐,毛小伟,肖调义,王红权,邓元元,谭斌,2013,菜籽粕混合菌固体发酵脱毒条件的响应面优化研究,动物营养学报,25(3):617-627)
Lee J.K.,Yun J.H.,Jeon J.K.,Kim S.K.,and Byunet H.G.,2010,Effect of antioxidant peptide isolated from brachionus calyciflorus,Journal of the Korean Society for Applied Biological Chemistry,53(2):192-197
Lee S.H.,Gupta M.K.,Bang J.B.,Bae H.,Hak-Joon Sung H.J.,2013,C urrent progress in reactive oxygen species(ROS)-responsive materials for biomedical applications,Advanced Healthcare Materials,2(6):908-915
Li S.R.,Lin X.J.,Cai H.S.,Hu K.H.,and Chen J.C.,2009,Preparing soybean peptides from soybean meal by mixed strain fermentation,Zhongguo Liangyou Xuebao(Journal of the Chinese Cereals and Oils Association),24(12):52-56(李善仁,林新坚,蔡海松,胡开辉,陈济琛,2009,混菌发酵豆粕制备大豆肽的研究,中国粮油学报,24(12):52-56)
Liu M.,Luo Y.X.,Ni H.,and Wu Y.P.,2007,Active analysis of soybean antioxidative peotide from solid state fermentation,Dadou Kexue(Soybean Science),26(3):381-385(刘明,罗远栩,倪辉,吴永沛,2007,固态发酵大豆制备的抗氧化肽的活性分析,大豆科学,26(3):381-385)
Li u S.,and Yu B.,2009,Peptides from variegated carp(Aristichthys nobilis)swim bladder:fermentation production and assessment of antioxidant properties,Shipin Kexue(Food Science),30(21):332-334(刘姝,余勃,2009,发酵法制备鱼鳔多肽及其抗氧化活性研究,食品科学,30(21):332-334)
Liu X.H.,2011,Studies on the character of soybean meal fermented by multi-microorganism,Thesis for M.S.,South China University of Technology,Supervisor:Zhao J.L.,pp.48-49(刘雪花,2011,混合菌株发酵豆粕及其特性研究,硕士学位论文,华南理工大学,导师:赵继伦,pp.48-49)
Lu G.Z.,Hou C.,Zhong Y.C.,Lu Y.,and Zou H.,2017,Recent progress of reactive oxygen species-responsive drug delivery systems,Yaoxue Xuebao(Acta Pharmaceutica Sinica),52(2):206-213(卢光照,侯成,钟延强,鲁莹,邹豪,2017,活性氧自由基响应给药系统研究进展,药学学报,52(2):206-213)
Ma L.H.,Qin W.D.,and Chen X.H.,2011,Comparative study on antioxidative activities of soybean peptide produced by different microbiological strains,Shipin Gongye Keji(Science and Technology of Food Industry),32(10):201-204(马利华,秦卫东,陈学红,2011,不同菌种制备大豆多肽的抗氧化活性比较研究,食品工业科技,32(10):201-204)
Mao H.Y.,Yang W.G.,Xu D.L.,Xie G.H.,and Zhang J.J.,2014,Preparation and molecular weigh t distribution of gelatin antioxidant peptides from jumbo flying squid(Dosidicus gigas)skin,Zhongguo Shipin Xuebao(Journal of Chinese Institute Of Food Science and Technology),14(9):48-55(茅宇虹,杨文鸽,徐大伦,谢果凰,张进杰,2014,秘鲁鱿鱼皮明胶抗氧化肽的制备及其分子质量分布,中国食品学报,14(9):48-55)
Peng H.H.,2012,Optimization of fermentation sesame meal conditions and antionxidant activity analysis of the sesame peptide,Thesis for M.S.,Anhui Agricultural University,Supervisor:Li L.M.,pp.35-37(彭惠惠,2012,芝麻粕发酵条件优化及其小肽抗氧化活性研究,硕士学位论文,安徽农业大学,导师:李吕木,pp.35-37)
Rajapakse N.,Mendis E.,Jung W.K.,Je J.Y.,and Kim S.K.,2005,Purification of a radical scavenging peptide from fermented mussel sauce and its antioxidant properties,Food Research Interational,38(2):175-182
Ren X.J.,2013,The preliminary studies on improving the quality of peanut meal with microbial fermentation,Thesis for M.S.,Kangnam University,Supervisor:Lu J.,pp.7-13(任晓静,2013,微生物发酵提高花生粕品质的初步研究,硕士学位论文,江南大学,导师:陆健,pp.7-13)
Wang Y.G.,Lin L.,Li M.H.,Weng S.B.,Ye Y.W.,Jiang S.T.,and Lu J.F.,2010,Enzymatic preparation of antioxidant peptides from channel catfish(Ictalurus punctatus)skin gelatin,Shipin Kexue(Food Science),31(19):254-258(王运改,林琳,李明辉,翁世兵,叶应旺,姜绍通,陆剑锋,2010,鮰鱼皮明胶抗氧化肽的制备工艺研究,食品科学,31(19):254-258)
Wei X.Y.,Yin L.Q.,Zhong C.,Zhang M.H.,and Niu Y.Z.,2014,Advances in the DPPH radical scavenging assay for antioxidant activity evaluat,Shipin Kexue(Food Science),35(9):317-322(韦献雅,殷丽琴,钟成,章明海,牛应泽,2014,DPPH法评价抗氧化活性研究进展,食品科学,35(9):317-322)
Williams G.M.,Iatropoulos M.J.,and Whysner J.,1999,Safety assessm ent of butylated hydroxyanisole and butylated hydroxytoluene as antioxid ant food additives,Food and Chemical Toxicology,37(9):1027-1038
Zhang Y.W.,2012,Research on antioxidant peptides prepared from fermented peanut meal by using Bacillus subtilis,Thesis for M.S.,Kangnam University,Supervisor:Zhang H.,pp.12-16(张友维,2012,枯草芽孢杆菌发酵花生粕制备抗氧化肽的研究,硕士学位论文,江南大学,导师:张晖,pp.12-16)
基本信息:
DOI：10.13417/j.gab.037.002068
中图分类号:TQ929;TS229
引用信息:
[1]袁艳超,贾泽,赖爱兰,等.混菌发酵芝麻粕制备芝麻多肽及其体外抗氧化活性研究[J].基因组学与应用生物学,2018,37(05):2068-2073.DOI:10.13417/j.gab.037.002068.
基金信息:
江西省农副产品绿色高值化利用创新团队项目(赣科发计字[2015]84号)资助
2018-05-25
2018-05-25