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近年来,随着食品安全事件频发,研究者们愈加重视发展低成本、高灵敏、易操作的现场快速检测技术。核酸适配体因其高特异性、高亲和力、易于修饰和功能化等优点而被广泛地应用于生物医学和分析化学研究领域,特别是核酸适配体的可视化检测技术备受关注。基于核酸适配体的可视化检测方法具有选择性好、特异强、灵敏度高、无需大型贵重仪器等诸多优点,已成为一种极具应用潜力的检测方法。本手稿主要对核酸适配体技术以及核酸适配体在食源性致病菌、真菌毒素、农兽药残留、重金属等食品有害物质的可视化检测研究进展进行综述。
Abstract：With frequent occurrence of food safety incidents, an on-site and rapid detection method with advantages like low cost, high sensitivity and easy processing has recently become one of focuses. Aptamer has been used widely in biomedical and analytical chemistry fields, because of its high specificity, affinity, easier modification and functionalization. Among them, the visual detection in the field of food safety using aptamer strategy is a matter of great concern. The visual detection based on aptamers has a number of advantages such as high selectivity, sensitivity and no large-scale instruments. It is a potential method in analysis and detection. This paper mainly reviewed nucleic acid aptamer technology and advances in visual detection with the application of aptamers of the harmful substances in food such as foodborne pathogens, mycotoxins, veterinary drug and pesticide residue,as well as heavy metals.
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基本信息:
DOI：10.13417/j.gab.035.002100
中图分类号:TS207.3
引用信息:
[1]谭贵良,赵旭,刘妍,等.核酸适配体在食品安全可视化检测中的应用研究进展[J].基因组学与应用生物学,2016,35(08):2100-2108.DOI:10.13417/j.gab.035.002100.
基金信息:
广东省高等学校优秀青年教师培养计划资助项目(Yq2013196);; 国家质检总局科技计划项目(2014QK051);; 广东省食药局科技计划项目(2015ZX07);; 中山市科技计划项目(2014A2FC247,2015B2353)共同资助
2016-08-31
2016-08-31
2016-08-31