| 550 | 24 | 64 |
| 下载次数 | 被引频次 | 阅读次数 |
具有碱性-螺旋-转角-螺旋结构的一大类基因被称为bHLH家族基因,其成员主要有c-MYC、MADL、MAX、MIXL和MNT基因;bHLH转录因子在细胞的增殖、分化和凋亡方面起着重要作用;对植物的生长发育、营养吸收、生物合成和信号转导等方面同样有着重要作用。在果肉、叶片和外果皮中,CsMYC2的表达与CHS、UFGT及ANS表达相关联,因此CsMYC2参与花色苷的生物合成调控,但是该基因在云南红皮梨花色苷合成调控中起着什么样的作用尚不清楚。利用生物信息学方法对云南红皮梨(Red Pyrus pyrifolia)bHLH基因进行分析,对不同植物间bHLH基因氨基酸序列的同源性比对,并且对该基因编码的蛋白质的理化性质、结构和功能等进行预测和分析。结果表明:该蛋白属于亲水性蛋白,其二级结构中的主要结构元件是α-螺旋和无规则卷曲,并散布于整个蛋白。这一结果为研究红皮梨bHLH基因的结构、功能以及红皮梨的着色机制奠定了一定的基础。
Abstract：The bHLH a basic-helix-turn-helix structure and its bHLH family members incloud c-MYC, MADL and MAX, MIXL and MNT. bHLH transcription factors play an important role in cell proliferation, differentiation and apoptosis. It also has a vital role on plant growth and development, nutrient absorption and biosynthesis, signal transduction, etc. In the flesh, leaves and exocarp, CsMYC2 expression was associated with CHS, UFGT and ANS expression. Therefore, CsMYC2 involved in anthocyanin biosynthesis and regulation. It is not clear what the gene plays a role of anthocyanin synthesis regulation in Yunnan red-skin pear. This research investigated the bHLH gene of Yunnan red-skin pears by using bioinformatic method and software. The homology of amino acid sequences of the bHLH among different plants was compared and the physical and chemical properties, structure and function of the gene encoding protein were predicted and analyzed. The result showed that the protein was hydrophilic without signal peptide with its second structure of the protein mainly containing α-helix and random coil, which spread randomly. The findings could be used to investigate the structure and function of the gene and to provide foundations for controlling effect of the bHLH gene in the coloration process of red-skin pears in further study.
Baudry A.,Heim M.A.,Dubreucq B.,Caboche M.,Weisshaar B.,and Lepiniec L.,2004,TT2,TT8,and TTG1 synergistically specify the expression of BANYULS and proanthocyanidin biosynthesis in Arabidopsis thaliana,The Plant Journal,39(3):366-380
Boter M.,Ruiz-Rivero O.,Abdeen A.,and Prat S.,2004,Conserved MYC transcription factors play a key role in jasmonate signaling both in tomato and Arabidopsis,Genes&Dev.,12(18):1577-1591
Buck M.J.,and Atchley W.R.,2003,Phylogenetic analysis of plant basic helix-loop-helix proteins,Journal of Molecular Evolution,56(6):742-750
Feng X.M.,2011,Cloning and functional characterization of an apple bHLH gene MdCIbHLH1,Dissertation for Ph.D.,Shandong Agriculture University,Supervisor:Hao Y.J.,pp.79 -87(冯晓明,2011,苹果bHLH基因MdCIbHLH1克隆及功能分析,博士学位论文,山东农业大学,导师:郝玉金,pp.79-87)
He K.P.,and Wu C.,2010,The effects of bHLH transcription factors on plant morphogenesis,Anhui Nongye Kexue(Journal of Anhui Agriculture Science),38(5):19957-19959(何开平,吴楚,2010,bHLH转录因子对植物形态发生的影响,安徽农业科学,38(35):19957-19959)
Hennig L.,Gruissem W.,Grossniklaus U.,and Kohler C.,2004,Transcriptional programs of early reproductive stages in Arabidopsis,Plant Physiology,135:1765-1775
Jiao Y.L.,Lau O.S.,and Deng X.W.,2007,Light-regulated transcriptional networks in higher plants,Nature Reviews Genetics,8(3):217-230
Ledent V.,and Vervoot M.,2001,The basic helix-loop-helix protein family:Comparative genomics and phylogenetic analysis,Genome Res.,11:754-770
Li X.X.,2006,Genome-wide analysis of basic/helix-loop-helix transcription factor family in rice and Arabidopsis,Thesis for M.S.,Xiamen University,Supervisors:Chen L.,and Zhang D.B.,pp.44-55(李晓星,2006,水稻和拟南芥中碱性/螺旋-环-螺旋转录因子家族基因组水平分析,硕士学位论文,厦门大学,导师:陈亮,张大兵,pp.44-55)
Li X.X.,Duan X.P.,Jiang H.X.,Sun Y.J.,Tang Y.P.,Yuan Z.,Guo J.K.,Liang W.Q.,Chen L.,Yin J.Y.,Ma H.,Wang J.,and Zhang D.B.,2006,Genome-wide analysis of basic/helix-loop-helix transcription factor family in rice and Ar-abidopsis,Plant Physiology,141(4):1167-1184
Lorenzo O.,Chico J.M.,Sanchez-Serrano J.J.,and Solano R.,2004,JASMONATE-INSENSITIVE1 encodes a MYC transcription factor essential to discriminate between different jasmonateregulated defense responses in Arabidopsis,The Plant Cell,16(7):1938-1950
Sakuma Y.,Maruyama K.,Osakabe Y.,Qin F.,Seki M.,Shinozaki K.,and Yamaguchi-Shinozaki K.,2006,Functional analysis of an Arabidopsis transcription factor,DREB2A,involved in drought-responsive gene expression,The Plant Cell,18(5):1292-1309
Spelt C.,Quattrocchio F.,Mol J.N.M.,and Koes R.,2000,anthocyaninl of petunia encodes a basic helix-loop-helix protein that directly activates transcription of structural antocyanin genes,Plant Cell,12(9):1619-1631
Toledo-Ortiz G.,Huq E.,and Quail P.H.,2003,The Arabidopsis basic/helix-loop-helix transcription factor family,The Plant Cell,15(8):1749-1770
Wang J.L.,Zhai L.J.,Chen J.,Gu H.Y.,and Chen Z.L.,2000,The evolutional regulation of CHS exon 2 and used to study the feasibility of plant molecular systematics,Kexue Tongbao(Chinese Science Bulletin),45(9):942-950(王金玲,瞿礼嘉,陈军,顾红雅,陈章良,2000,CHS基因外显子2的进化规律及其用于植物分子系统学研究的可行性,科学通报,45(9):942-950)
Wang Y.J.,Zhang Z.G.,He X.J.,Zhou H.L.,Wen Y.X.,Dai J.X.,Zhang J.S.,and Chen S.Y.,2003,A rice transcription factor OsbHLH1 is involved in cold stress response,Theoretical Applied Genetics,107(8):1402-1409
Zhang Q.Q.,Zhu J.H.,Ni Y.M.,and Zhang Z.L.,2011,The structure and function of plant bHLH transcription factors,Redai Yanredai Zhiwu Xuebao(Journal of Tropical and Subtropical Botany),19(1):84-90(张全琪,朱家红,倪燕妹,张治礼,2011,植物bHLH转录因子的结构特点及其生物学功能,热带亚热带植物学报,19(1):84-90)
Zhang W.B.,and Pu Y.W.,1997,The red pear germplasm resources of Yunnan and their utilization,Zhongguo Nanfang Guoshu(South China Fruits),26(5):38-39(张文炳,普亚文,1997,云南红皮梨种质资源及其利用,中国南方果树,26(5):38-39)
Zhang W.B.,and Zhang J.R..,1993,The red pear varieties resources in subtropical regions of Yunnan,Zhongguo Guoshu(China Fruits),(4):16-17(张文炳,张俊如,1993,云南亚热带地区的红色梨品种资源,中国果树,(4):16-17)
Zhao L.,2011,Influence of bHLH homologous gene expression on flower coloration of chrysanthemum,Thesis for M.S.,Beijing Forestry University,Supervisor:Dai S.L.,pp.22-25(赵莉,2011,bHLH同源基因的表达对菊花花色的影响,硕士学位论文,北京林业大学,导师:戴思兰,pp.22-25)
Zhao M.Z.,Morohashi K.,Hatlestad G.,Grotewold E.,and Lloyd A.,2008,The TTG1-bHLH-MYB complex controls trichome cell fate and patterning through direct targeting of regulatory loci,Development,135(11):1991-1999
Zhou J.,Li F.,Wang J.L.,Ma Y.,Chong K.,and Xu Y.Y.,2009,Basic helix-loop-helix transcription factor from wild rice(OrbHLH2)improves tolerance to salt and osmotic stress in Arabidopsis,Journal of Plant Physiology,166(12):1296-1306
基本信息:
中图分类号:S661.2
引用信息:
[1]孟富宣,周军,辛培尧,等.云南红皮梨bHLH转录因子的生物信息学分析[J].基因组学与应用生物学,2013,32(05):652-659.
基金信息:
国家林业局948项目“苹果抗性品种及分子辅助育种技术引进”(2013-4-44);; 西南山地森林资源保育与利用省部共建教育部重点实验室开放基金项目(BC2010F08);; 国家林业局重点实验室开放基金(BC2010F08);; 云南省省院省校教育合作咨询共建重点学科林学和西南林业大学重点建设学科林木遗传育种(XKX-200904)共同资助
2013-10-28
2013-10-28