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2006, S1, 149-155
卵母细胞胞浆内精子注射(ICSI)技术的研究进展
基金项目(Foundation): 国家自然科学基金项目(30270647,30571332);; 教育部留学回国人员科研启动基金项目
邮箱(Email):
DOI:
摘要:

利用人工辅助的显微操作技术,直接将精子注射至成熟卵母细胞质的方法,称为胞浆内精子注射技术,简称ICS I(in tracytop lasm ic sperm in jection)技术。目前,该项技术已经成功地用于生产试管婴儿、研究哺乳动物的受精机理、动物转基因、家畜性控胚胎生产等领域。本文详述了ICS I技术体系的发展背景、现状及其应用等方面。

Abstract:

Intracytoplasmic sperm injection(ICSI) has become the method of choice to overcome male infertility,to study the molecular events of fertilization,to produce the transgenic animals and sexing livestock.The review described the history of ICSI technique and its biology and applications in humans and animals.

参考文献

[1]DENG M,YANG X J.Fu ll term deve lopm en t of rabb it oocytes fertilized by in tracytop lasm ic sperm in jection[J].M o l R eprod D ev,2001,59:38-43.

[2]GOM EZ M C,POPE C E,HARR IS R,et a l.B irths of k ittens produced by in tracytop lasm ic sperm in jection ofdom estic cat oocytes m atured in v itro[J].R eprod F ertil D ev,2000,12:423-433.

[3]GOM EZ M C,CATT JW,EVAN S G,et a l.C leavage,deve lopm en t and com petence of sheep em bryos fertilizedby in tracytop lasm ic sperm in jection and in v itro fertilization[J].T heriogeno logy,1998,49:1143-1154.

[4]GOTO K,YANAG ITA K.N orm a lity of ca lves ob ta ined by in tracytop lasm ic sperm in jection[J].Hum R eprod,1995,10:1554.

[5]PALERM O G,JOR IS H,DEVROEY P,et a l.P regnanc ies after in tracytop lasm ic in jection of s ing le sperm atozoonin to an oocyte[J].L ancet,1992,340:17-18.

[6]K IMURA Y,YANAG IM ACH I R.In tracytop lasm ic sperm in jection in the m ouse[J].B io l R eprod,1995,52:709-720.

[7]M ART IN M J.D eve lopm en t of in v ivo-m atured porc ine oocytes fo llow ing in tracytop lasm ic sperm in jection[J].B io l R eprod,2000,63:109-112.

[8]L I X,M ORR IS L H,ALLEN W R.In fluence of co-cu lture during m aturation on the deve lopm en ta l poten tia l ofequ ine oocytes fertilized by in tracytop lasm ic sperm in jection(ICS I)[J].R eproduction,2001,121:925-932.

[9]YAM AUCH I Y,YANAG IM ACH I R,HOR IUCH I T.Fu ll-term deve lopm en t of go lden ham ster oocytes fo llow ingin tracytop lasm ic sperm head in jection[J].B io l R eprod,2002,67:534-539.

[10]HEW ITSON L,DOM INKO T,TAKAHA SH I D,et a l.U n ique checkpo in ts during the first ce ll cycle of fertilizationafter in tracytop lasm ic sperm in jection in rhesus m onkeys[J].N atM ed,1999,5:431-433.

[11]H IRABAYA SH I M,KATO M,ISH IKAW A A,et a l.O ffspring derived from in tracytop lasm ic in jection oftransgen ic rat sperm[J].T ransgen ic R es,2002,11:221-228.

[12]W ANG B,BALDA SSARRE H,P IERSON J,et a l.T he in v itro and in v ivo deve lopm en t of goat em bryos producedby in tracytop lasm ic sperm in jection us ing ta il-cu t sperm atozoa[J].Zygote,2003,11:219-227.

[13]YAN A Y,L I M,AN X R,et a l.D eve lopm en t norm a l m ice from oocytes fo llow ing in tracytop lasm ic spermin jection(ICS I)[J].Ch in J B iotechno l,2005,21(2):301-310.

[14]K IMURA Y,YANAG IM ACH I R.M ouse oocytes in jected w ith testicu lar sperm atozoa or round sperm atids candeve lop in to norm a l offspring[J].D eve lopm en t,1995,121:2 397-2 405.

[15]K IMURA Y,YANAG IM ACH I R.D eve lopm en t of norm a l m ice from oocytes in jected w ith secondarysperm atocyte nucle i[J].B io l R eprod,1995,53:855-862.

[16]KU SAKABE H,SZCZYG IEL M A,WH ITT INGHAM D G,et a l.M a in tenance of genetic in tegrity in frozen andfreeze-dried m ouse sperm atozoa[J].P roc N atl A cad Sc iU SA,2001,98:13501-13506.

[17]W AKAYAM A T,YANAG IM ACH I R.D eve lopm en t of norm a l m ice from oocytes in jected w ith freeze-driedsperm atozoa[J].N at B iotechno l,1998,16:639-641.

[18]PERRY A C,W AKAYAM A T,K ISH IKAW A H,et a l.M amm a lian transgenes is by in tracytop lasm ic spermin jection[J].Sc ience,1999,284:1 180-1 183.

[19]KATO M,ISH IKAW A A,KANEKO R,et a l.P roduction of transgen ic rats by oop lasm ic in jection ofsperm atogen ic ce lls exposed to exogenous DNA:a pre lim inary study[J].M o l R eprod D ev,2004,69:153-158.

[20]PERRY A C,ROTHM AN A,LA S HERA S J I,et a l.E ffic ien tm etaphaseⅡtransgenes is w ith d ifferen t transgenearchetypes[J].N at B iotechno l,2001,19:1 071-1 073.

[21]M ORE IRA P N,G IRALDO P,COZAR P,et a l.E ffic ien t generation of transgen ic m ice w ith in tact yeast artific ia lchrom osom es by in tracytop lasm ic sperm in jection[J].B io l R eprod,2004,71:1943-1947.

[22]L IM,YAN A Y,YAO H,et a l.T ransgen ic M ice P roduced by In tracytop lasm ic Sperm In jection[J].Ch in JB iotechno l,2006,22(5):784-789.

[23]TESAR IK J.C a lc ium in oocyte m aturation.How the sperm atozoon aw akens the oocyte:lessons fromin tracytop lasm ic sperm in jection[J].Hum R eprod,1994,9:977-978.

[24]NAKANO Y,SH IRAKAW A H,M ITSUHA SH I N,et a l.Spatiotem pora l dynam ics of in trace llu lar ca lc ium in them ouse egg in jected w ith a sperm atozoon[J].M o lHum R eprod,1997,3:1087-1093.

[25]W ARD W S,K IMURA Y,YANAG IM ACH I R.A n in tact sperm nuclear m atrix m ay be necessary for the m ousepaterna l genom e to partic ipate in em bryon ic deve lopm en t[J].B io l R eprod,1999,60:702-706.

[26]KATAYAM A M,KO SH IDA M,M IYAKE M.F ate of the acrosom e in oop lasm in p igs after IVF and ICS I[J].Hum R eprod,2002,17:2657-2664.

[27]RAM ALHO-SANTO S J,S IM ERLY C R,HEW ITSON L,et a l.A crosom e com ponen ts after in tracytop lasm icsperm in jection:the decondensation fron tier[J].F ertil S teril,2001,76:196-197.

[28]K IMURA Y,YANAG IM ACH I R,KURETAKE S,et a l.A na lys is of m ouse oocyte activation suggests theinvo lvem en t of sperm perinuclear m ateria l[J].B io l R eprod,1998,58:1407-1415.

[29]M OROZUM IK,YANAG IM ACH IR.Incorporation of the acrosom e in to the oocyte during in tracytop lasm ic spermin jection cou ld be poten tia lly hazardous to em bryo deve lopm en t[J].P roc N atlA cad Sc iU SA,2 0 0 5,1 0 2:14209-14214.

[30]KURETAKE S,K IMURA Y,HO SH I K,et a l.F ertilization and deve lopm en t of m ouse oocytes in jected w ithiso lated sperm heads[J].B io l R eprod,1996,55:789-795.

[31]NAVARA C S,HEW ITSON L C,S IM ERLY C R,et a l.T he im p lications of a paterna lly derived cen trosom eduring hum an fertilization:consequences for reproduction and the treatm en t of m a le factor in fertility[J].Am JR eprod Imm uno l,1997,37:39-49.

[32]SCHATTEN G,S IM ERLY C,SCHATTEN H.M icrotubu le con figurations during fertilization,m itos is,and earlydeve lopm en t in the m ouse and the requ irem en t for egg m icrotubu le-m ed iated m otility during m amm a lianfertilization[J].P roc N atl A cad Sc iU SA,1985,82:4152-4156.

[33]Z IYYAT A,LEFEVRE A.D ifferen tia l gene express ion in pre-im p lan tation em bryos from m ouse oocytes in jectedw ith round sperm atids or sperm atozoa[J].Hum R eprod,2001,16:1449-1456.

[34]HAYA SH I S,YANG J,CHR ISTEN SON L,et a l.M ouse pre im p lan tation em bryos deve loped from oocytes in jectedw ith round sperm atids or sperm atozoa have s im ilar bu t d istinct patterns of early m essenger RNA express ion[J].B io l R eprod,2003,69:1170-1176.

[35]K IMURA Y,TATENO H,HANDEL M A,et a l.F actors affecting m e iotic and deve lopm en ta l com petence ofprim ary sperm atocyte nucle i in jected in to m ouse oocytes[J].B io l R eprod,1998,59:871-877.

[36]M ANN ING M,L ISSEN S W,BONDUELLE M,et a l.S tudy of DNA-m ethy lation patterns at chrom osom e 15q11-q13 in ch ildren born after ICS I revea ls no im prin ting defects[J].M o lHum R eprod,2000,6:1049-1053.

[37]DEBAUN M R,N IEM ITZ E L,FE INBERG A P.A ssoc iation of in v itro fertilization w ith B eckw ith-W iedem annsyndrom e and ep igenetic a lterations of L IT 1 and H 19[J].Am J Hum G enet,2003,72:156-160.

[38]M AHER E R.Im prin ting and ass isted reproductive techno logy[J].Hum M o l G enet,2005,14(1):133-138.

[39]YOUNG L E,FERNANDES K,M CEVOY T G,et a l.Ep igenetic change in IGF 2R is assoc iated w ith feta lovergrow th after sheep em bryo cu lture[J].N at G enet,2001,27:153-154.

[40]M ARQUES C J,CARVALHO F,SOU SA M,et a l.G enom ic im prin ting in d isruptive sperm atogenes is[J].L ancet,2004,363:1700-1702.

[41]SCHULTZ R M,W ILL IAM S C J.T he sc ience of ART[J].Sc ience,2002,296:2188-2190.

[42]BHATTACHARYA S,HAM ILTON M P,SHAABAN M,et a l.Conven tiona l in-v itro fertilisation versusin tracytop lasm ic sperm in jection for the treatm en t of non-m a le-factor in fertility:a random ised con tro lled tria l[J].L ancet,2001,357:2075-2079.

[43]KAW A SE Y,IW ATA T,TOYODA Y,et a l.Com parison of in tracytop lasm ic sperm in jection for inbred and hybridm ice[J].M o l R eprod D ev,2001,60:74-78.

[44]KURETAKE S,M ALESZEW SK IM,TOKUM A SU A,et a l.Inadequate function of sterile tw 5/tw 32 sperm atozoaovercom e by in tracytop lasm ic sperm in jection[J].M o l R eprod D ev,1996,44:230-233.

[45]AKUTSU H,TRES L L,TATENO H,et a l.O ffspring from norm a lm ouse oocytes in jected w ith sperm headsfrom the azh/azh m ouse d isp lay m ore severe sperm ta il abnorm a lities than the orig ina lm u tan t[J].B io l R eprod,2001,64:249-256.

[46]M ENG X,AKUTSU H,SCHOENE K,et a l.T ransgene insertion induced dom inan t m a le sterility and rescue ofm a le fertility us ing round sperm atid in jection[J].B io l R eprod,2002,66:726-734.

[47]H IRABAYA SH IM,KATO M,AOTO T,et a l.R escue of in fertile transgen ic rat lines by in tracytop lasm ic in jectionof cryopreserved round sperm atids[J].M o l R eprod D ev,2002,62:295-299.

[48]YANAG IM ACH I R.In tracytop lasm ic sperm in jection experim en ts us ing the m ouse as a m ode l[J].Hum R eprod,1998,13(Supp l 1):87-98.

[49]W ARD M A,KANEKO T,KU SAKABE H,et a l.Long-term preservation of m ouse sperm atozoa after freeze-dry ing and freez ing w ithou t cryoprotection[J].B io l R eprod,2003,69:2100-2108.

[50]KANEKO T,WH ITT INGHAM D G,YANAG IM ACH I R.E ffect of pH va lue of freeze-dry ing so lu tion on thechrom osom e in tegrity and deve lopm en ta l ab ility of m ouse sperm atozoa[J].B io l R eprod,2003,68:136-139.

[51]CATT S L,CATT JW,GOM EZ M C,et a l.B irth of a m a le lam b derived from an in v itrom atured oocyte fertilisedby in tracytop lasm ic in jection of a s ing le presum ptive m a le sperm[J].V et R ec,1996,139:494-495.

[52]HAM ANO K,L I X,Q IAN X Q,et a l.G ender prese lection in cattle w ith in tracytop lasm ica lly in jected,flowcytom etrica lly sorted sperm heads[J].B io l R eprod,1999,60:1194-1197.

[53]PROBST S,RATH D.P roduction of p ig lets us ing in tracytop lasm ic sperm in jection(ICS I)w ith flow cytom etrica llysorted boar sem en and artific ia lly activated oocytes[J].T heriogeno logy,2003,59:961-973.

基本信息:

中图分类号:Q813

引用信息:

[1]刘占才,陈学进,严阿勇,等.卵母细胞胞浆内精子注射(ICSI)技术的研究进展[J].广西农业生物科学,2006(S1):149-155.

基金信息:

国家自然科学基金项目(30270647,30571332);; 教育部留学回国人员科研启动基金项目

发布时间:

2006-12-30

出版时间:

2006-12-30

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