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Krüppel样转录因子15 (Krüppel-like factors,KLF15)是Krüppel样转录因子家族的一员。Krüppel样转录因子家族的特征是含有3个高度保守的Cys2His2锌指结构,与DNA的CACCC元件和富含GC区连接,从而调控转录激活或抑制。KLF15在糖、脂肪酸、氨基酸代谢过程中发挥重要的调控作用。本研究克隆了尼罗罗非鱼(Oreochromis niloticus) KLF15基因全长cDNA序列,根据推测的氨基酸序列分析,发现其具有KLF15的两个典型结构域zf-H2C2-2和zf-C2H2。系统进化树的比较分析表明,尼罗罗非鱼KLF15与雪鲷(Maylandia zebra) KLF15亲缘关系最为接近。尼罗罗非鱼短期饥饿实验结果显示:正常进食状态时尼罗罗非鱼肌肉、肝中KLF15基因都呈现明显的昼夜节律性,均为昼低夜高;饥饿7 d后KLF15基因依然有显著昼夜节律性,但振幅降低,且在肝中表现出昼高夜低现象。以上结果说明尼罗罗非鱼的营养变化在一定程度上直接或间接影响KLF15基因节律性表达。
Abstract：Krüppel-like factor 15(KLF15) is a member of the Krüppel-like zinc-finger transcription factor family.The Krüppel-like zinc-finger transcription factor family is characterized by three highly conserved zinc finger structures,Cys2 His2,linked to the CACCC elements and GC-rich regions of DNA,which regulates transcriptional activation or repression.KLF15 plays an important regulatory role in the metabolism of glucose,fatty acids,and amino acids.The full-length c DNA sequence of KLF15 gene was cloned from tilapia and two typical domains,zf-H2 C2-2 and zf-C2 H2,were found at present study.The genetic relationship between the KLF15 gene of Nile tilapia and the KLF15 gene of Maylandia zebra was found to be the closest based on the comparative analysis of phylogenetic trees.The results of short-term hunger experiments show that the KLF15 genes in the muscles and livers exhibit circadian rhythm during normal feeding state in Nile tilapia,and the expression of KLF15 gene in muscle and liver is higher during the day than at night;the KLF15 gene is still significantly rhythmic after 7 days of hunger,however,the amplitude is reduced,and the expression of KLF15 gene in liver is higher at night than during the day.The nutritional change was found to have a certain degree of direct or indirect effect on the rhythmic expression of KLF15 gene in Nile tilapia.
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基本信息:
DOI：10.13417/j.gab.039.000051
中图分类号:S917.4
引用信息:
[1]安晓玲,张建社,褚武英,等.饥饿对尼罗罗非鱼肌肉和肝KLF15基因节律性表达的影响[J].基因组学与应用生物学,2020,39(01):51-58.DOI:10.13417/j.gab.039.000051.
基金信息:
国家自然科学基金面上项目(31472256)和(31572592)共同资助
2018-10-24
2018-10-24
2018-10-24