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为探究文冠果(Xanthoceras sorbifolium Bunge)性别分化过程的分子机制,本研究对其性别分化关键期花芽进行RNA-seq分析。结果共获得37.13 Gb原始数据,de novo组装后共产生29 430条Unigenes,平均长度1 144 bp,其中21 887条Unigenes获得功能注释。此外,共得到差异基因4 864个,其中相对于性别分化前期(Ta)差异上调基因3 003个,差异下调基因1 861个。GO富集分析显示,差异基因主要注释到代谢进程、植物激素信号转导、生长素激活的信号通路等功能。KEGG富集分析显示,差异基因主要注释到植物激素信号转导、淀粉和蔗糖代谢等生物功能。还筛选出6个MADS-box相关基因(TR576|c0_g1, TR7438|c0_g1,TR10037|c1_g10, TR13325|c0_g1, TR6316|c0_g1, TR5807|c0_g1),参与雄蕊发育、花粉管生长、花发育调节、花瓣发育、胚珠发育、转录因子活性等过程调节。RT-qPCR检测了16个基因在两个时期的表达量,结果均与RNA-seq一致。研究结果将为进行文冠果性别分化过程中基因表达分析提供参考。
Abstract：In order to explore the molecular mechanism of sex differentiation process in Xanthoceras sorbifolium Bunge, flower buds at sex differentiation key stage were sampled to perform RNA-seq analysis. As a result, a total of 37.13 Gb raw data was obtained. After de novo assembly, a total of 29 430 Unigenes were produced with an average length of 1 144 bp, of which 21 887 Unigenes were functionally annotated. In addition, 4 864 differential genes were obtained. Compared with pre-sex differentiation stage(Ta), 3 003 genes were up-regulated, and 1 861 genes were down-regulated. GO enrichment analysis showed that differential genes were mainly annotated to metabolic processes, plant hormone signal transduction, auxin-activated signaling pathways, and so on. KEGG enrichment analysis demonstrated differential genes were mainly annotated to biological functions such as plant hormone signal transduction, starch and sucrose metabolism. Six MADS-box related genes(TR576|c0＿g1, TR7438|c0＿g1, TR10037|c1＿g10, TR13325|c0＿g1, TR6316|c0＿g1, TR5807|c0＿g1) were screened involving stamen development, pollen tube growth, flower development regulation, petal development, process regulation of ovule development, transcription factor activity, and so on. The expression levels of 16 genes in two periods were detected by RT-qPCR, and the results were consistent with RNA-seq. The results of the present study would provide a reference for the research on the gene expression analysis in sex differentiation in X. sorbifolium.
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基本信息:
DOI：10.13417/j.gab.040.001254
中图分类号:S565.9
引用信息:
[1]郑雅琪,敖妍,王旭,等.文冠果性别分化关键期花芽转录组分析[J].基因组学与应用生物学,2021,40(03):1254-1267.DOI:10.13417/j.gab.040.001254.
基金信息:
中央高校基本科研业务费专项资金(2015ZCQ-LX-02; 2019GJZL02); 青年科学基金项目(31600241)共同资助
2019-11-26
2019-11-26
2019-11-26