| 408 | 11 | 67 |
| 下载次数 | 被引频次 | 阅读次数 |
本研究以1株野生离褶伞属真菌为研究对象,通过扩增rD NA-ITS序列并测序分析后确定该野生菌株为暗褐离褶伞(Lyophyllum loricatum)。进一步对该菌株进行生物学特性研究,结果表明,其菌丝可生长温度范围为10~35℃,最适生长温度为25℃,可生长的pH范围为4.09.5,最适生长pH为5.5,最适生长碳源为麦芽糖,最适生长氮源为蛋白胨,最适生长因子为肌醇。本研究对暗褐离褶伞菌株的初步探索,为暗褐离褶伞的驯化栽培提供了重要的理论基础。
Abstract：Our research analysized a wild fungus strain through the amplification and sequencing of its rD NA-ITS,and based on that we verified it as Lyophyllum loricatum.Additional biological characteristics analysis showed that its mycelia could grow from 10~35℃ and its optimal temperature was 25℃; it could grow ranging from 4.09.5 and its optimal temperature was 5.5; its optimal carbon source was maltose,the optical nitrogen source was peptone and the optimal growth factor was inositol.This research supplied preliminary exploration on Lyophyllum loricatum,laying crucial theoretical base for its domestication and cultivation.
Chen J.S.,and Zheng F.C.,2007,Application of ITS sequences infungi classification and identification,Anhui Nongye Kexue(Journal of Anhui Agricultural Sciences),35(13):3785-3786(陈剑山,郑服丛,2007,ITS序列分析在真菌分类鉴定中的应用,安徽农业科学,35(13):3785-3786)
Cheng J.H.,Zheng H.F.,Ben W.D.,and Ma S.Z.,2008,Industrialcultivation of Lyophyllum decastes,Shiyongjun Xuebao(ActaEdulis Fungi),15(2):20-22(程继红,郑慧芬,贲伟东,马书智,2008,荷叶离褶伞工厂化栽培,食用菌学报,15(2):20-22)
Gui J.H.,Wei S.L.,and Wang S.L.,2011,A taxonomic study ongenus Lyophyllum in Qilian mountain nature reserve,CaoyeXuebao(Acta Pratacul Turae Sinia),20(5):284-287(桂建华,魏生龙,王生龙,2011,祁连山自然保护区离褶伞属真菌资源,草业学报,20(5):284-287)
Landeweert R.,Leeflang P.,Kuyper T.W.,Hoffland E.,Rosling A.,Wernars K.,and Smit E.,2003,Molecular identification ofectomycorrhizal mycelium in soil horizons,Appl.Environ.Microbiol.,69(1):327-333
Li X.,and Li Y.,2009,Present status and future prospects ofLyophyllum.Karst.in China,Shiyongjun Xuebao(Acta Edulis Fungi),16(3):75-79(李晓,李玉,2009,中国离褶伞属真菌研究进展,食用菌学报,16(3):75-79)
Tao Y.X.,Xie B.G.,Yang Z.Y.,Chen Z.H.,Chen B.Z.,Deng Y.J.,Jiang Y.J.,and van Peer A.F.,2013,Identification andexpression analysis of a new glycoside hydrolase family 55exo-茁-1,3-glucanase-encoding gene in Volvariella volvaceasuggests a role in fruiting body development,Gene,527(1):154-160
Wang S.X.,Liu Y.,Xu F.,Zhao S.,Geng X.L.,Wang L.Q.,andMeng L.L.,2012,Study on identification and biological characteristics of wild Lyophyllum decastes strain,ZhongguoNongxue Tongbao(Chinese Agricultural Science Bulletin),28(28):148-152(王守现,刘宇,许峰,赵爽,耿小丽,王兰青,孟莉莉,2012,荷叶离褶伞菌株的ITS鉴定及生物学特性研究,中国农学通报,28(28):148-152)
Wang X.Q.,Zhou D.Q.,Zhang X.L.,and Li S.H.,2013,Taxonomic summarization on the genus Lyophyllum,ZhongguoShiyongjun(Edible Fungi of China),32(1):1-3(汪小庆,周德群,张小雷,李树红,2013,离褶伞属分类学研究概况,中国食用菌,32(1):1-3)
Wang X.Q.,Zhou D.Q.,Zhang X.L.,Li L.,and Li S.H.,2013,Lyophyllum rhombisporum sp.nov.from China,Mycotaxon,123(5):473-477
Wei S.L.,Wang Z.J.,Yu H.P.,and Chen Y.,2006,Biologicalcharacteristics of Lyophyllum decastes(Fr.:Fr.)Singer,Junwu Xuebao(Mycosystema),25(1):101-108(魏生龙,王治江,于海萍,陈叶,2006,荷叶离褶伞生物学特性研究,菌物学报,25(1):101-108)
Yang Y.B.,Lan J.X.,Xie F.Q.,Zhang K.J.,and Yi H.,2010,Phylogeny tree analysis of Lentinus edodes based on rD NA-ITSsequences,Weishengwuxue Zazhi(Journal of Microbiology),(4):27-29(杨永彬,兰家细,谢福泉,张孔金,羿红,2010,基于香菇菌株rD NA-ITS序列的系统发育分析,微生物学杂志,(4):27-29)
Zhang S.L.,Chen Y.,and Chen L.,2013,The technology aboutseparating spore from edible fungi,Heilongjiang NongyeKexue(Heilongjiang Agricultural Sciences),(11):114-115(张术丽,陈影,陈亮,2013,食用菌单孢分离技术,黑龙江农业科学,(11):114-115)
基本信息:
DOI：10.13417/j.gab.034.000106
中图分类号:S646
引用信息:
[1]郑秋霞,蒋木杉,应顺秋,等.一株野生离褶伞菌株的ITS序列分析及其生物学特性的研究[J].基因组学与应用生物学,2015,34(01):106-111.DOI:10.13417/j.gab.034.000106.
基金信息:
国家支撑计划项目(2013bad16b03)资助
2015-01-25
2015-01-25