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水稻化感作用指水稻通过向环境中释放次生代谢产物来影响周围植物的生长。本研究以国际公认的化感水稻PI312777和非化感水稻Lemont为材料,克隆肉桂酸-4-羟化酶(C4H)编码基因CYP73A38的cDNA序列进行对比分析,发现两种水稻在该基因的序列差异极小,存在6个位点差异。为了进一步揭示两种水稻化感潜力不同的原因,对CYP73A38启动子进行了克隆分析,获得CYP73A38基因启动子序列,该启动子序列包含CAAT-box、TATA-box、光响应元件、赤霉素响应元件、生长素响应元件、低温响应元件等多个顺式作用元件及MYB类转录因子结合位点,且差异位点均不在顺式作用元件上,由此推测两种水稻化感潜力的差异与基因结构没有相关性。不同化感潜力水稻CYP73A38基因及其启动子的克隆与序列分析,将为进一步研究CYP73A38基因的表达调控及其启动子功能分析提供参考,为加快水稻化感抗性品种的选育进程提供依据。
Abstract：Rice alle lopathy is mainly responsible for the growth of around plant by releasing secondary metabolites into the environment. In this study, allelopathic rice PI312777 and non-allelopathic rice Lemont were used as materials, cloned the cinnamic acid-4-hydroxylase(C4H) coding gene CYP73A38 full length of sequence cDNA and comparatived difference. The differences in the sequence of this gene in allelopathic and non-allelic rice were slight. A total of 6 nucleotide variations were found in the ORFs. In order to research the reason of two rice with different allelopathic potential, the study cloned the promoter sequence of CYP73A38. The promoter sequence contained multiple cis-acting elements, such as CAAT-box, TATA-box, light responsive element, gibberellins responsive element, auxin responsive element, low-temperature responsive element, MYB recognition site, and others. And all variation sites are not in the cis-acting elements. Thus speculate that the differences of two allelopathy potential rice do es not correlate with the genetic structure. Cloning and characterization of the CYP73A38 gene and its promoter will provide basis for the further study of regulation and functional analysis of CYP73A38 gene in rice and the breeding process of allelopathic resistant rice varieties.
Bak S.,Beisson F.,Bishop G.,Hamberger B.,Hofer R.,Paquette S.,and Werck-Reichhart D.,2011,Cytochromes P450,The Arabidopsis Book,2011:e0144
Betz C.,Mccollum T.G.,and Mayer R.T.,2001,Differential ex pression of two cinnamate 4-hydroxylase genes in"Valencia"orange(Citrus sinensis Osbeck),Plant Mol.Biol.,46(6):741-748
Chen D.W.,Wang X.,Li H.Y.,Tang Z.A.,Tang Y.H.,Tao J.,and Zhao D.Q.,2018,Cloning of C4H gene in herbaceous peony and expression characteristic analysis of It under calcium treatment,Fenzi Zhiwu Yuzhong(Molecular Plant Breeding),16(9):2795-2801(陈德伟,王昕,李红叶,汤梓岸,汤寓涵,陶俊,赵大球,2018,芍药C4H基因的克隆及其在钙处理下的表达特性分析,分子植物育种,16(9):2795-2801)
Cho M.H.,and Lee S.W.,2015,Phenolic phytoalexins in rice:biological functions and biosynthesis,Int.J.Mol.Sci.,16(12):29120-29133
Duke S.O.,2010,Allelopathy:Current status of research and future of the discipline:a commentary,Allelopathy J.,25(1):17-30
Fang C.X.,Zhuang Y.E.,Xu T.C.,Li Y.Z.,Li Y.,and Lin W.X.,2013,Changes in rice allelopathy and rhizosphere microflora by inhibiting rice phenylalanine ammonia-lyase gene expression,J.Chem.Ecol.,39(2):204-212
Frank M.R.,Deyneka J.M.,and Schuler M.A.,1996,Cloning of wound-induced cytochrome P450 monooxygenases expressed in pea,Plant Physiol.,110(3):1035-1046
Guo Y.Q.,and Lu Y.L.,2015,Rice allelopathy to weed and weed biological control,Zhongguo Shengwu Fangzhi Xuebao(Chinese Journal of Biological Control),31(2):157-165(郭怡卿,陆永良,2015,水稻化感作用与杂草的生物防治,中国生物防治学报,31(2):157-165)
Inderjit,1996,Plant phenolics in allelopathy,Bot.Rev.,62(2):186-202
Kato-Noguchi H.,Ota K.,and Kujime H.,2012,Absorption of momilactone A and B by Arabidopsis thaliana L.and the growth inhibitory effects,J.Plant Physiol.,169(15):1471-1476
Kawahara Y.,de la Bastide M.,Hamilton J.P.,Kanamori H.,McCombie W.R.,Ouyang S.,Schwartz D.C.,Tanaka T.,Wu J.,Zhou S.,Childs K.L.,Davidson R.M.,Lin H.,Quesada-O-campo L.,Vaillancourt B.,Sakai H.,Lee S.S.,Kim J.,Numa H.,Itoh T.,Buell C.R.,and Matsumoto T.,2013,Improvement of the Oryza sativa Nipponbare reference genome using next generation sequence and optical map data,Rice,6(1):4
Kong C.H.,Xu X.H.,Hu F.,Chen X.H.,Ling B.,and Tan Z.W.,2002,Using specific secondary metabolites as markers to evaluate allelopathic potentials of rice varieties and individual plants,Chinese Sci.Bull.,47(10):839-843
Koopmann E.,Logemann E.,and Hahlbrock K.,1999,Regulation and functional expression of cinnamate 4-hydroxylase from parsley,Plant Physiol.,119(1):49-56
Lin W.X.,Fang C.X.,Chen T.,Lin R.Y.,Xiong J.,and Wang H.B.,2010,Rice allelopathy and its properties of molecular ecology,Front.Biol.,5(3):255-262
Macias F.A.,Chinchilla N.,Varela R.M.,and Molinillo J.M.G.,2006,Bioactive steroids from Oryza sativa L.,Steroids,71(7):603-608
Mizutani M.,Ohta D.,and Sato R.,1997,Isolation of a cDNAand a genomic clone encoding cinnamate 4-hydroxylase from Arabidopsis and its expression manner in planta,Plant Physiol.,,113(3):755-763
Wang H.B.,Zhang Z.X.,Li H.,He H.B.,Fang C.X.,Zhang A.J.,Li Q.S.,Chen R.S.,Guo X.K.,Lin H.F.,Wu L.K.,Lin S.,Chen T.,Lin R.Y.,Peng X.X.,and Lin W.X.,2011,Characterization of metaproteomics in crop rhizospheric soil,J.Proteome Res.,10(3):932-940
Xiong J.,Lin H.F.,Li Z.F.,Fang C.X.,Han Q.D.,and Lin W.X.,2012,Analysis of rhizosphere microbial community structure of weak and strong allelopathic rice varieties under dry paddy field,Shengtai Xuebao(Acta Ecologica Sinica),32(19):6100-6109(熊君,林辉锋,李振方,方长旬,韩庆典,林文雄,2012,旱直播条件下强弱化感潜力水稻根际微生物的群落结构,生态学报,32(19):6100-6109)
Yu L.,Zhang Y.,Chen A.G.,Liang H.B.,Qamaruddin J.,Xu W.L.,and Xu N.,2014,Isolation and expression analysis of Ntc4h and Nt4cl encoding the key enzymes of phenylalanine metabolism pathway in tobacco,Zhiwu Yichuan Ziyuan Xuebao(Journal of Plant Genetic Resources),15(5):1067-1073(于利,张彦,陈爱国,梁洪波,Qamaruddin Jogi,徐文玲,许娜,2014,烟草苯丙烷代谢途径关键酶肉桂酸-4-羟化酶、4-香豆酸-辅酶A基因的分离及表达特性分析,植物遗传资源学报,15(5):1067-1073)
基本信息:
DOI：10.13417/j.gab.039.004073
中图分类号:S511
引用信息:
[1]杨奕,孙一丁,马继琼,等.两种不同化感潜力水稻CYP73A38基因及其启动子的克隆分析[J].基因组学与应用生物学,2020,39(09):4073-4081.DOI:10.13417/j.gab.039.004073.
基金信息:
云南省科技计划青年项目(2017FD204)资助
2020-09-25
2020-09-25