| 221 | 9 | 55 |
| 下载次数 | 被引频次 | 阅读次数 |
为开发栎属遗传多样性检测的SSR标记,分析了蒙古栎、无梗花栎、夏栎和欧洲栓皮栎EST-SSR的特点,结果表明蒙古栎的EST中3 209.46 bp有一个SSR,无梗花栎中每6 160.36 bp有一个SSR、夏栎中每5 883.30 bp有一个SSR,欧洲栓皮栎中每6 129.12 bp有一个SSR。蒙古栎、无梗花栎、夏栎和欧洲栓皮栎EST-SSR的平均长度分别为21.65 bp、21.1 bp、20.66 bp和20.65 bp。四种栎类中不同基元的EST-SSR的分布频率具有非常一致的特征,均是二基元、三基元和六基元的SSR分布频率最高,达20%以上。而四基元和五基元的SSR在四个种类中的分布不到0.05%。二基元的SSR中大于1%的SSR均是AG、CT、TC、GA、AT、TA基元,并且在蒙古栎、无梗花栎和夏栎中AG、CT、TC基元的分布频率最高,而在欧洲栓皮栎中是TC、GA、AG的分布频率最高;三基元的SSR中,含CAA、GAA、TCT、CTT的SSR在四种栎类中都存在。六基元的SSR中大于1%在四种栎类中出现的类型均较少,为04种。
Abstract：In order to develop SSR marks for genetic diversity detection in Quercus,the characters of EST-SSR of Quercus mogolica,Quercus petraea,Quercus robur and Quercus suber were estimated.Results showed the average SSR density was one SSR per 3 209.46 bp of Q.mogolica,one SSR per 6 160.36 bp of Q.petraea,one SSR per5 883.30 bp of Q.petraea,one SSR per 6 129.12 bp of Q.suber,and the average SSR length was 21.65 bp,21.1 bp,20.66 bp,and 20.65 bp,respectively.The dinucleotide,trinucleotide repeats and the hex nucleotide repeats were the most abundant detected of the four species,the value of each kind of the three repeats type accounted for more than20%.However,the tetranucleotide pentanucleotide repeats accounted for less than 0.05%.AG,CT,TC,GA,AT,and TA motifs were the dinucleotide repeats that detected in all the four species.AG,CT,TC motifs were the most abundant in Q.mogolica,Q.petraea,Q.robur,and in Q.suber TC,GA,AG were the most abundant.Among the trinucleotide repeats CAA,GAA,TCT,CTT motifs were detected in all the four species.The type of hexnucleotide repeats that accounted for more than 1% in the four species was small,with 04 types only.
An Z.W.,Zhao Y.H.,Cheng H.,Li W.G.,and Huang H.S.,2009,Development and application of EST-SSR markers in Hevea brasiliensis Muell.Arg,Yichuan(Hereditas),31(3):311-319(安泽伟,赵彦宏,程汉,李维国,黄华孙,2009,橡胶树EST-SSR标记的开发与应用,遗传,31(3):311-319)
Cardle L.,Ramsay L.,Milbourne D.,Macaulay M.,Marshall D.,and Waugh R.,2000,Computational and experimental characterization of physically clustered simple sequence repeats in plants,Genetics,156(2):847-854
Cordeiro G.M.,Casu R.,McIntyre C.L.,and Henry R.J.,2001,Microsatellite markers from sugarcane(Saccharum spp.)ESTs cross transferable to erianthus and sorghum,Plant Science,160(6):1115-1123
Dow B.D.,Ashley M.V.,and Howe H.F.,1995,Characterization of highly variable(GA/CT)n microsatellites in the bur oak,Quercus macrocarpa,Theoretical and Applied Genetics,91(1):137-141
Dow B.D.,and Ashley M.V.,1996,Microsatellite analysis of seed dispersal and parentage of samplings in bur oak,Quercus macrocarpa,Molecular Ecology,l5:615-627
Fan H.H.,Li T.C.,Li Z.P.,Lin Y.,and Cai Y.P.,2009,Characteristics of EST-SSR distribution in Ginkgo ESTs,Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),28(5):869-873(樊洪泓,李廷春,李正鹏,林毅,蔡永萍,2009,银杏EST序列中微卫星的分布特征,基因组学与应用生物学,28(5):869-873)
Feng S.P.,Li W.G.,Huang H.S.,Wang J.Y.,and Wu Y.T.,2009,Development,application and cross-species/genera transferability of EST-SSR makers for rubber tree(Hevea brasiliensis),Molecular Breeding,23(1):85-97
Fraser L.G.,Harvey C.F.,Crowhurst R.N.,and de Silva H.N.,2004,EST-derived microsatellites from Actinidia species and their potential for mapping,Theoretical and Applied Genetics,108(6):1010-1016
Gao L.F.,Tang J.F.,Li H.W.,and Jia J.Z.,2003,Analysis of microsatellites in major crops assessed by computational and eperimental approaches,Molecular Breeding,12(3):245-261
Gupta P.K.,Rustgi S.,Sharma S.,Singh R.,Kumar N.,and Balyan H.S.,2003,Transferable EST-SSRs markers for the study of polymorphism and genetic diversity in bread wheat,Molecular Genetics and Genomics,270(4):315-323
Jin J.Q.,Cui H.R.,Chen W.Y.,Lu M.Z.,Yao Y.L.,Xin Y.,and Gong X.C.,2006,Data mining for SSRs in ESTs and development of EST-SSR marker in tea plant(Camellia sinensis),Caiye Kexue(Journal of Tea Science),26(1):17-23(金基强,崔海瑞,陈文岳,卢美贞,姚艳玲,忻雅,龚晓春,2006,茶树EST-SSR的信息分析与标记建立,茶叶科学,26(1):17-23)
Kampfer S.,Lexer C.,Glssl J.,and Steinkellner H.,1998,Characterization of(GA)n microsatellite loci from Quercus robur,Hereditas,129(2):183-186
Kantety R.V.,Rota M.L.,Matthews D.E.,and Sorrells M.E.,2002,Data mining for simple sequence repeats in expressed sequence tags from barley,maize,rice,sorghum and wheat,Plant Molecular Biology,48(5-6):501-510
Li S.X.,and Yin T.M.,2007,Map and analysis of microsatellites in genome of Populus:The first sequenced perennial plant,Science in China C:Life Sciences,50(5):690-699
Li S.X.,Zhang X.Y.,Wang Y.Y.,and Yin T.M.,2010,Content and characteristics of microsatellites detected in expressed sequence tag sequences in Eucalyptus,Zhiwu Xuebao(Chinese Bulletin of Botany),45(3):363-371(李淑娴,张新叶,王英亚,尹佟明,2010,桉树EST序列中微卫星含量及相关特征,植物学报,45(3):363-371)
Steinkellner H.,Lexer C.,Turetschek E.,and Glssl J.,1997,Conservation of(GA)n microsatellite loci between Quercus species,Molecular Ecology,6(12):1189-1194
Sun Q.M.,Ma W.C.,Ma S.P.,Zhao J.S.,Bai L.J.,Chen J.Z.,Cai C.H.,Xiang X.,and Ou L.X.,2011,Characteristics of SSRs derived from ESTs and development of EST-SSR markers in Litchi(Litchi chinensis Sonn.),Zhongguo Nongye Kexue(Scientia Agricultura Sinica),19:4037-4049(孙清明,马文朝,马帅鹏,赵俊生,白丽军,陈洁珍,蔡长河,向旭,欧良喜,2011,荔枝EST资源的SSR信息分析及EST-SSR标记开发,中国农业科学,19:4037-4049)
Wang J.,Zhao M.Z.,Yu H.M.,and Wang Z.W.,2011,Develop ment and utilization of EST-SSR marker in strawberry,Guoshu Xuebao(Journal of Fruit Science),28(4):721-726(王静,赵密珍,于红梅,王壮伟,2011,草莓EST-SSR标记的开发与应用,果树学报,28(4):721-726)
Wang Y.M.,Wei Z.G.,and Yang C.Q.,2008,Data mining for SSRs in ESTs and EST-SSR marker development in Betula platyphyllab,Linye Kexue(Scientia Silvae Sinicae),44(2):78 -84(王艳敏,魏志刚,杨传平,2008白桦EST-SSR信息分析与标记的开发,林业科学,44(2):78-84)
Xu Y.L.,Cai N.H.,Kang X.Y.,Zhang R.L.,Wang D.W.,He C.Z.,and Duan A.A.,2012,Development and characterization of EST-SSR markers in woody plants,Zhongguo Nongxue Tongbao(Chinese Agricultural Science Bulletin),28(04):1-7(许玉兰,蔡年辉,康向阳,张瑞丽,王大玮,何承忠,段安,2012,EST-SSR标记的开发及其在木本植物中的分布特点,中国农学通报,28(04):1-7)
Xiang W.,Ning Z.,Kang M.,and Huang H.W.,2010,Characteristic and generality of EST-SSRs of Castanea mollissima,Redai Yanredai Zhieu Xuebao(Joumal of Tropical and Subtropical Botany),18(6):665-669(向闱,宁祖林,康明,黄宏文,2010,中国板栗EST-SSR信息分析及其通用性,热带亚热带植物学报,18(6):665-669)
Zhang R.H.,Li R.,Zhao J.K.,and Xu L.A.,2011,Development and population detection of microsatellite markers from expressed sequence tags for Tamarix spp.,Fenzi Zhiwu yuzhong(Molecular Plant Breeding),9:1291-1296(张如华,李锐,赵景奎,徐立安,2011,柽柳EST-SSRs标记开发与群体检测,分子植物育种,9:1291-1296)
Zhu Y.,Hao Y.B.,Wang K.J.,Wu C.L.,Wang W.X.,Qi J.X.,and Zhou J.,2009,Analysis of SSRs information and development of SSR markers from walnut ESTs,Guoshu Xuebao(Journal of Fruit Science),26(3):394-398(朱艳郝,艳宾,王克建,吴春林,王维霞,齐建勋,周军,2009,核桃EST-SSR信息分析与标记的初步建立,果树学报,26(3):394-398)
基本信息:
DOI：10.13417/j.gab.033.000133
中图分类号:S792.186
引用信息:
[1]张元燕,方炎明.四种栎树EST-SSR信息分析[J].基因组学与应用生物学,2014,33(01):133-140.DOI:10.13417/j.gab.033.000133.
基金信息:
国家自然科学基金项目(31370666);; 江苏高校优势学科建设工程资助项目(PAPD)共同资助
2014-02-28
2014-02-28