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利用RNA-seq技术筛选影响肉质鲜味的相关差异基因，运用实时荧光定量PCR (realtime quantita tive PCR, RT-qPCR)技术对宁夏地方品种静原鸡胸肌和腿肌组织中已筛选的与肉质鲜味相关基因的m RNA表达量进行测定，结果表明：静原鸡胸肌和腿肌之间的显著差异表达基因为666个，其中显著上调的基因有256个(P<0.05)，显著下调的基因有410个(P<0.05);GO功能注释和KEGG通路分析显示，在嘌呤代谢、丙氨酸、天门冬氨酸和谷氨酸代谢通路中筛选出与肌苷酸相关的差异表达基因腺苷琥珀酸合成酶同工酶1基因(adenylosuccinate synthetase isozyme 1, ADSSL1)，且ADSSL1在胸肌中mRNA表达量极显著高于腿肌(P<0.01)。本试验结果为研究肌苷酸相关候选基因的筛选提供了科学依据，同时为后续深入了解ADSSL1基因提供了理论参考。
Abstract：The differential genes affecting meat quality and taste were screened by RNA-seq, and the expression of genes related to meat taste in breast muscle and leg muscle of Jingyuan chicken were verified by RT-qPCR. The results showed that there were 666 genes significantly differentially expressed between breast muscle and leg muscle of Jingyuan chicken, of which 256 genes were significantly up-regulated and 410 genes were significantly down-regulated(P<0.05). GO functional annotation and KEGG pathway analysis showed that adenylosuccinate synthetase isozyme 1(ADSSL1), a differentially expressed gene related to inosinic acid, was screened from purine metabolism, alanine, aspartic acid and glutamate metabolism pathways, and the m RNA expression of ADSSL1 in chest muscle was significantly higher than that in leg muscle(P<0.01). The results of this study provide a scientific basis for the screening of inosinic acid-related candidate genes, and provide a theoretical reference for further understanding of ADSSL1 genes.
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基本信息:
DOI：10.13417/j.gab.040.002476
中图分类号:S831
引用信息:
[1]郭菊,邓占钊,周子航,等.基于RNA-seq技术对静原鸡ADSSL1基因的筛选及其表达量的分析[J].基因组学与应用生物学,2021,40(Z2):2476-2483.DOI:10.13417/j.gab.040.002476.
基金信息:
国家自然科学基金项目(31860621)
2021-08-25
2021-08-25