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山茱萸为中国常用的药食同源的中药材之一,是很多复方中药的主要成分,同时也是园林绿化的常用树种,应用非常广泛。近几年,山茱萸在降血糖、降血脂以及抗血栓等方面的功效备受关注。为深入研究b HLH类转录因子参与调控山茱萸有效成分合成途径的分子机制,本研究在对山茱萸果实开展转录组测序的前提下,以转录本unigene c98416_g2为参考序列,设计特异引物,通过RT-PCR技术,扩增山茱萸的b HLH34转录因子,命名为Cob HLH34,采用DNAstar、Protparatam和psipred等在线分析软件,解析该序列的分子组成、蛋白的分子量以及二级结构预测,结果表明:山茱萸Cob HLH34的c DNA序列长度为740 bp,其ORF序列编码232个氨基酸,理论等电点为7.95,α-螺旋和无规则卷曲是Cob HLH34蛋白的主要组成部分。基于NCBI-Blast比对和MEGA 6.0构建的系统进化树显示,就Cob HLH34而言,山茱萸与橡胶树、胡桃和狭叶羽扇豆聚为一支,具有较高的同源性。本研究有助于探讨Cob HLH34的功能。
Abstract：Cornus officinalis is one of the homology of medicine and food,which is the main ingredient of many compound Chinese medicines.At the same time,it also is the common tree species in landscaping and widely used.In recent years,Cornus officinalis paid much attention on its function of decreasing blood sugar,depressing blood-fat and anti-thrombosis.In order to study the molecular mechanism of b HLH in regulating the biosynthesis pathway of active constituent from Cornus officinalis,the special primers were designed using the unigene c98416_g2,based on the transcriptome sequencing of Cornus officinalis fruits.The transcription factor b HLH34 was cloned through the RT-PCR method,which called Cob HLH34.The molecular component,protein molecular weight and secondary structure were analyzed using the software DNAstar,protparatam and psipred.The results showed that the c DNA of Cob HLH34 was 740 bp in length,encoded 423 amino acid.The isoelectric point was7.95.The main structure of Cob HLH34 are alpha-helix and random coil.The phylogenetic tree basing on theNCBI-Blast and MEGA 6.0 indicated that there was the one cluster among Cornus officinalis,Hevea brasiliensis,Juglans regia and Lupinus angustifolius,which had the highly homologous.The result would be beneficial to the functional study of Cornus officinalis.
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基本信息:
DOI：10.13417/j.gab.037.003460
中图分类号:Q943.2;S567.19
引用信息:
[1]侯典云,李炯,杨萌萌,等.基于转录组测序的山茱萸转录因子CobHLH34的克隆与分析[J].基因组学与应用生物学,2018,37(08):3460-3465.DOI:10.13417/j.gab.037.003460.
基金信息:
国家自然科学基金项目(U1404829);; 河南省教育厅自然科学研究计划重点项目(14A180006);河南省教育厅自然科学研究计划重点项目(12B180007)共同资助
2018-08-24
2018-08-24