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为了探讨BoLA-DRA基因多态性,本研究利用DNA混合池扩增产物直接测序的方法对荷斯坦牛BoLA-DRA基因编码区SNPs进行筛选,并利用生物信息学软件预测该基因m RNA二级结构。结果表明:荷斯坦牛BoLA-DRA基因编码区共有4个SNPs (exon2-A82T,exon2-G116A,exon2-C197T,exon2-C233A)。生物信息学预测结果显示,exon2-A82T、exon2-G116A和exon2-C233A增大了m RNA二级结构的稳定性,而exon2-C197T降低了mRNA二级结构的稳定性。本研究结果可为荷斯坦牛抗病和抗逆性能研究积累更多的分子遗传学数据,并为经济性状相关基因筛选提供理论依据。
Abstract：To discuss polymorphism of BoLA-DRA gene,the single nucleotide polymorphism site(SNPs) of coding region of BoLA-DRA gene in Holstein cattle were screened by DNA pooled amplification and directly sequencing,and further secondary structure of m RNA were predicted based on the bioinformatics software.The results showed that 4 SNPs(exon2-A82 T,exon2-G116 A,exon2-C197 T and exon2-C233 A) were detected in Holstein cattle.The results of bioinformatics showed exon2-A82 T,exon2-G116 A and exon2-C233 A increased the stability of secondary structure of m RNA,while exon2-C197 T reduced the stability of secondary structure of mRNA.The results could accumulate more molecular genetic data for research of disease resistance and resisting performance,and provide theoretical basis for selection of economic trait correlation genes.
Amorena B.,and Stone W.H.,1978,Serologically defined(SD)locus in cattle,Science,201(4351):159-160
Andersson L.,Bohme J.,Peterson P.A.,and Rask L.,1986,Genomic hybridization of bovine classⅡmajor histocompatibility genes:2.Polymorphism of DR genes and linkage disequilibrium in the DQ-DR region,Anim.Genet.,17(2):295-304
An T.W.,Zhang H.,He J.W.,Feng H.Y.,Luo Y.Z.,and Han J.L.,2012,Polymorphic but highly conserved Bogr-DRA gene in yak(Bos grunniens),Anim.Genet.,43(2):237-238
Carignano H.A.,Beribe M.J.,Caffaro M.E.,Amadio A.,Nani J.P.,Gutierrez G.,Alvarez I.,Trono K.,Miretti M.M.,and Poli M.A.,2017,BoLA-DRB3 gene polymorphisms influence bovine leukaemia virus infection levels in Holstein and Holstein×Jersey crossbreed dairy cattle,Anim.Genet.,48(4):420-430
Fries R.,Hediger R.,and Stranzinger G.,1986,Tentative chromosomal localization of the bovine major histocompatibility complex by in situ hybridization,Anim.Genet.,17(2):287-294
Gao S.X.,Xu S.Z.,Gao X.,Ma Y.,and Han Y.G.,2008,Analysis of polymorphisms of BoLA-DRA*exon2 and the relationship between the gene and mastitis in two dual-purpose breed cattle,Huabei Nongxuebao(Acta Agriculturae Boreali-Sinica),23(1):211-213(高树新,许尚忠,高雪,马云,韩玉国,2008,两种兼用型牛BoLA-DRA*exon2多态性及其与乳房炎的关联分析,华北农学报,23(1):211-213)
Gowane G.R.,Sharma A.K.,Vandre R.K.,and Sankar M.,2017,Association of DRB 3 exon 2 alleles with productive and reproductive performance of crossbred cattle,J.Vet.Res.,3(1):31-35
Gu L.J.,Ren L.Q.,Yan Z.H.Zhang Y.Y.,Song G.X.,Tian S.J.,Liu H.J.,Yang X.J.,and Yang T.B.,2015,Polymorphism and bioinformatics analysis of ADRP gene in pigs of Zongdihua and Congjiangxiang,Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),34(11):2395-2401(顾丽菊,任丽群,燕志宏,张依裕,宋高翔,田松军,刘华钧,杨秀江,杨通斌,2015,宗地花猪和从江香猪ADRP基因多态性及生物信息学分析,基因组学与应用生物学,34(11):2395-2401)
Jamshidi J.,Movafagh A.,Emamalizadeh B.,Zare Bidoki A.,Manafi A.,Ghasemi Firouzabadi S.,Shahidi G.A.,Kazeminasab S.,Petramfar P.,Fazeli A.,Motallebi M.,Mortazavi Tabatabaei S.A.,Kowsari A.,Jafarian Z.,and Darvish H.,2014,HLA-DRA is associated with Parkinson's disease in I-ranian population,Int.J.Immunogenet.,41(6):508-511
Li C.M.,Shi W.H.,Chu M.X.,An Y.F.,Chen H.Q.,Di R.,and Fang L.,2009,Polymorphism of exon 2 of bovine lymphocyte antigen-DRB3 gene(BoLA-DRB3)and its relationship with somatic cell score in Holstein cattle,Nongye Shenwu Jishu Xuebao(Journal of Agricultural Biotechnology),17(4):593-598(李春苗,石万海,储明星,安永福,陈宏权,狄冉,方丽,2009,荷斯坦牛白细胞抗原基因-DRB3(BoLA-DRB3)外显子2多态性及其与体细胞评分的关系,农业生物技术学报,17(4):593-598)
Li Y.Q.,Niu X.L.,Hu J.,Yan W.,Luo Y.,Wang W.B.,and Cairang N.R.,2015,Polymorphism of BoLA-DRA*Exon2-intron2 in Yak and Bovine,Huabei Nongxuebao(Acta Agriculturae Boreali-Sinica),30(4):72-77(李彦清,牛晓亮,胡江,闫伟,罗玉柱,王文飙,才让闹日,2015,牦牛和普通牛BoLA-DRA*Exon2-intron2多态性分析,华北农学报,30(4):72-77)
Sena L.,Schneider M.P.C.,Brenig B.,Honeycutt R.L.,Womack J.E.,and Skow L.C.,2003,Polymorphisms in MHC-DRAand-DRB alleles of water buffalo(Bubalus bubalis)reveal different features from cattle DR alleles,Anim.Genet.,34(1):1-10
Shook G.E.,1993,Genetic improvement of mastitis through selection on somatic cell count,Vet.Clin.North Am.Food Anim.Pract.,9(3):563-577
Takeshima S.,Sasaki S.,Meripet P.,Sugimoto Y.,and Aida Y.,2017,Single nucleotide polymorphisms in the bovine MHCregion of Japanese Black cattle are associated with bovine leukemia virus proviral load,Retrovirol.,14(1):24
Trowsdale J.,1993,Genomic structure and function in the MHC,Trends Genet.,9(4):117-122
van der Poel J.J.,Groenen M.A.M.,Dijkhof R.J.M.,Ruyter D.,and Giphart M.J.,1990,The nucleotide sequence of the bovine MHC classⅡalpha genes:DRA,DQA,and DYA,Immunogenetics,31(1):29-36
Wang X.P.,Xu S.Z.,Zan L.S.,Chen J.B.,Gao X.,and Ren H.Y.,2007,Polymorphism and sequence analysis of the exon 2 of BoLA-DRB3 in cattle,Nongye Shenwu Jishu Xuebao(Journal of Agricultural Biotechnology),15(1):46-51(王兴平,许尚忠,昝林森,陈金宝,高雪,任红艳,2007,牛BoLA-DRB3基因第2外显子多态性及其序列分析,农业生物技术学报,15(1):46-51)
Wang K.,Zhang Y.,and Song D.X.,2008,Analysis of sequences of BoLA-DRB3 genes in Chinese Yellow cattle,Xumu Shouyi Xuebao(Acta Veterinaria et Zootechnica Sinica),39(7):853-857(王昆,张沅,孙东晓,2008,部分中国黄牛BoLA-DRB3基因序列分析,畜牧兽医学报,39(7):853-857)
Xu J.L.,Wang Y.,Hou M.,and Li Q.,2015,Progresson detection methods of SNP,Fenzi Zhiwu Yuzhong(Molecular Plant Breeding),13(2):475-482(许家磊,王宇,后猛,李强,2015,SNP检测方法的研究进展,分子植物育种,13(2):475-482)
Zambrano Arteaga J.C.,Echeverri Zuluaga J.,and López Herrera A.,2016,Characterization of the genetic polymorphism of the BoLA DRB3.2 gene in Holstein and Bonholstein cows from a dairy herd in Antioquia,Revista Lasallista de Investigación,13(1):76-84
基本信息:
DOI：10.13417/j.gab.039.000059
中图分类号:S823.2
引用信息:
[1]刘丽霞,乔自林,张丽,等.荷斯坦牛BoLA-DRA基因SNPs筛选及生物信息学分析[J].基因组学与应用生物学,2020,39(01):59-63.DOI:10.13417/j.gab.039.000059.
基金信息:
国家自然科学基金地区项目(31660628);; 西北民族大学中央高校基本科研业务费专项资金资助项目(31920-170029);西北民族大学“双一流”引导专项生物工程特色学科(10018703,1001070204)共同资助
2020-01-25
2020-01-25