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水稻(Oryza sativa L.)是世界上需求量最大的粮食来源之一，其产量和品质与分蘖和花序结构的发育有关。以往的花序发育研究已经鉴定出大量与水稻营养生长和生殖生长相关的基因，但对花序发育和抽穗期的遗传机制尚未完全了解。本研究利用前期构建的水稻小穗异常发育表型的重组自交系(recombinant inbred lines, RIL),通过转录表达谱分析，探讨穗部异常发育和穗部缺陷(pds1)表型调控的分子机制。通过差异表达基因(differentially expressed genes, DEGs)的筛选，鉴定了可能与pds1表型相关的基因。在6组差异表达基因中，分别鉴定出1 578、 463、 1 295、 687、 397、 175个差异表达基因。本研究通过加权共表达网络分析(weighted gene co-expression network analysis, WGCNA),发现了与pds1表型高度相关的4个基因模块(Module)。通过对模块中所包含的基因进行功能富集分析，发现与花序发育相关的生物学过程。随后在这些模块中鉴定出多个高度相关的关键基因(Hub genes),如开花促进因子OsFKF1(Os11g0547000)和TCP转录因子OsTB1(Os03g0706500)。结果表明，水稻小穗异常发育和抽穗缺陷表型可能是由小穗发育调控相关基因的遗传互作引起的。研究结果为进一步探讨穗伸长缺陷的调控机制提供了有价值的参考。
Abstract：Rice(Oryza sativa L.) is one of the largest food sources in the world, and its yield and quality are related to tillering and inflorescence structure development. Previous studies on inflorescence development have identified a large number of genes related to vegetative growth and reproductive growth of rice, but the genetic mechanism of inflorescence development and heading date has not been fully understood. In this study, we used the previously constructed recombinant inbred line(RIL) with spikelet abnormal development phenotype to explore the molecular mechanism of spike abnormal development and spike defect(pds1) phenotype regulation through transcriptional expression profile analysis. Through the screening of differentially expressed genes(DEGs), the genes possibly related to pds1 phenotype were identified. Among the six groups of differentially expressed genes, 1 578, 463, 1 295, 687, 397 and 175 differentially expressed genes were identified respectively. Through weighted gene co-expression network analysis(WGCNA), four gene modules highly correlated with pds1 phenotype were found in this study. Through the function enrichment analysis of the genes included in each module, the biological processes related to inflorescence development were idendified. Then, several highly correlated hub genes were identified in these modules, such as flowering promoting factor OsFKF1(Os11 g0547000) and TCP transcription factor OsTB1(Os03 g0706500). The results showed that the abnormal development of spikelets and the phenotype of heading defects in rice might be caused by the genetic interaction of genes related to spikelet development regulation. This provides a valuable reference for further exploring the regulation mechanism of panicle elongation defect.
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基本信息:
DOI：10.13417/j.gab.041.001732
中图分类号:S511
引用信息:
[1]吴迪,曾尹帝,罗继景.水稻小穗异常发育调控关键基因的加权共表达网络分析[J].基因组学与应用生物学,2022,41(08):1732-1742.DOI:10.13417/j.gab.041.001732.
基金信息:
广西自然科学基金面上项目(2019GXNSFAA245007)资助
2022-03-30
2022-03-30
2022-03-30