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本研究基于宏基因组鸟枪法测序技术，将一例辽宁朝阳地区太岁样本的测序数据与Micro＿NR、KEGG、eggNOG、CAZy数据库进行比对注释，对其微生物群落结构和功能基因谱进行系统解析。物种注释显示：细菌在界水平占绝对优势；门水平上，假单胞菌门（Pseudomonadota）相对丰度约达91.33%，是绝对优势菌门；属水平上，短波单胞菌属（Brevundimonas，约占21.18%）和水栖菌属（Aquincola，约占11.50%）为优势菌属；在种水平分类单元，叔碳水栖菌（Aquincola tertiaricarbonis）相对丰度约为10.33%，为本例辽宁朝阳太岁的优势菌种，α-变形菌纲菌种（Alphaproteobacteria bacterium）为辽宁地区太岁特有菌种。功能注释表明：KEGG通路中新陈代谢相关基因占比最高，双组分系统（ko02020）和ABC转运蛋白（ko02010）显著富集；eggNOG分类中功能未知（S）、氨基酸代谢（E）、转录调控（K）等类别丰度最高；CAZy数据库中糖基转移酶（glycosyl transferases， GT）家族与糖苷水解酶（glycoside hydrolases， GH）家族占主导。综上，本例辽宁朝阳太岁样本是以假单胞菌门为绝对主导、代谢活跃且具有复杂调控网络的微生物聚集体，其功能基因特征与物种组成高度一致，提示该样品可能具备高效的多糖代谢潜力。
Abstract：This study employed metagenomic shotgun sequencing technology to systematically analyze the microbial community structure and functional gene profile of a Taisui sample collected from Chaoyang， Liaoning Province. The sequencing data were aligned and annotated against the Micro＿NR， KEGG， eggNOG， and CAZy databases. Taxonomic annotation revealed that Bacteria dominated at the kingdom level. At the phylum level， Pseudomonadota exhibited a relative abundance of approximately 91.33%， making it the absolute dominant phylum. At the genus level， Brevundimonas (approximately 21.18%) and Aquincola (approximately 11.50%) were the dominant genera. At the species-level taxon， Aquincola tertiaricarbonis showed a relative abundance of approximately 10.33%， representing the dominant bacterial species in this Taisui sample from Chaoyang， Liaoning， while Alphaproteobacteria bacterium was identified as a species potentially endemic to Taisui from the Liaoning region. Functional annotation indicated that among KEGG pathways， metabolism-related genes were the most abundant， with significant enrichment of the two-component system (ko02020) and ABC transporters (ko02010). In the eggNOG classification， categories of unknown function (S)， amino acid transport and metabolism (E)， and transcription (K) were the most abundant. In the CAZy database， glycosyl transferases (GT) and glycoside hydrolases (GH) families predominated. In summary， the Taisui sample from Chaoyang， Liaoning Province is a microbial aggregate absolutely dominated by Pseudomonadota， metabolically active， and possessing a complex regulatory network. Its functional gene characteristics are highly consistent with its species composition， suggesting that this sample may have a high potential for efficient polysaccharide metabolism.
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基本信息:
中图分类号:Q933
引用信息:
[1]李松桥,班翔,徐建友,等.辽宁朝阳太岁微生物群落结构的宏基因组测序及功能基因解析[J].基因组学与应用生物学().
基金信息:
国家重点研发计划(2020YFE0201600)资助
2026-06-24
2026-06-24
2026-06-24