| 147 | 5 | 13 |
| 下载次数 | 被引频次 | 阅读次数 |
冠突曲霉是茯砖茶发花阶段的主要微生物,其在Na Cl作为渗透压调节下分别进入有性产孢和无性产孢两个不同的世代,为了考查钙信号途径在其特殊产孢过程中的作用,通过克隆冠突曲霉钙信号调控的关键基因钙调素cam基因,并对这一基因进行结构和表达分析。结果表示:冠突曲霉中的cam基因全长924 bp,开放阅读框为450 bp,含6个内含子,预测编码149个氨基酸。cam参与了冠突曲霉的产孢及盐压力响应过程,cam基因对其无性发育产生正调控。实验结果为钙信号调控丝状真菌盐压力应答下的产孢机制研究奠定基础。
Abstract：The Aspergillus cristatus was the dominant fungus in the Fuzhuan tea,A.cristatus possess the asexual development stage in 3 mol/L Na Cl and sexual development stage in 0.5 mol/L Na Cl.In order to explore whether the Ca2+signaling pathway responds to the changes in the salt stress in A.cristatus,cam gene of A.cristatus was cloned and characterized.The full length of cam gene c DNA of A.cristatus is 924 bp,including a 450 bp open reading frame encoding 149 amino acids.The expression levels of cam gene are up-regulated in the asexual stage.The study results provide the basis for further exploring the relationship between the salt stress response and spore germination mechanism in fungi.
Adamik ova L.,Straube A.,Schulz I.,and Steinberg G.,2004,Calcium signaling is involved in dynein-dependent microtubule organization,Mol.Biol.Cell,15(4):1969-1980
Ahn I.P.,Kim S.,Choi W.B.,and Lee Y.H.,2003,Calcium restores prepenetration morphogenesis abolished by polyamines in Colletotrichum gloeosporioides infecting red pepper,FEMS Microbiol.Lett.,227(2):237-241
Chen S.,Song Y.,Cao J.,Wang G.,Wei H.,Xu X.,and Lu L.,2010,Localization and function of calmodulin in live-cells of Aspergillus nidulans,Fungal Genet.Biol.,47(3):268-278
Chin D.,and Means A.R.,2000,Calmodulin:a prototypical calcium sensor,Trends Cell Biol.,10(8):322-328
Cruz M.C.,Fox D.S.,and Heitman J.,2001,Calcineurin is required for hyphal elongation during mating and haploid fruiting in Cryptococcus neoformans,EMBO J.,20(5):1020-1032
Cui J.,Kaandorp J.A.,Ositelu O.O.,Beaudry V.,Knight A.,Nanfack Y.F.,and Cunningham K.W.,2009,Simulating calcium influx and free calcium concentrations in yeast,Cell Calcium,45(2):123-132
Cyert M.S.,2001,Genetic analysis of calmodulin and its targets in Saccharomyces cerevisiae,Annu.Rev.Genet.,35(10):647-672
Greene V.,Cao H.,Schanne F.A.,and Bartelt D.C.,2002,Oxidative stress-induced calcium signalling in Aspergillus nidulans,Cell Signal,14(5):437-443
Harel A.,Bercovich S.,and Yarden O.,2006,Calcineurin is required for sclerotial development and pathogenicity of Sclerotinia sclerotiorum in an oxalic acid-independent manner,Mol.Plant Microbe Interact,19(6):682-693
Kahl C.R.,and Means A.R.,2003,Regulation of cell cycle progression by calcium/calmodulin-dependent pat hways,Endocr.Rev.,24(6):719-736
Kraus P.R.,and Heitman J.,2003,Coping with stress:calmodulin and calcineurin in model and pathogenic fungi,Biochemical and Biophysical Research Communications,311(4):1151-1157
Kumar R.,and Tamuli R.,2014,Calcium/calmodulin-dependent kinases are involved in growth,thermotolerance,oxidative stress survival,and fertility in Neurospora crassa,Arch.Microbiol.,196(4):295-305
Lee S.C.,and Lee Y.H.,1998,Calcium/calmodulin-dependent signaling for appressorium formation in the plant pathogenic fungus Magnaporthe grisea,Mol.Cells,8(6):698-704
Liu R.,Tan Y.M.,Liu Y.X.,and Liu Z.Y.,2015,Function assay of esd C gene in Aspergillus chevalieri var.intermedius,Junwu Xuebao(Mycosystema),34(2):227-234(刘荣,谭玉梅,刘永翔,刘作易,2015,谢瓦氏曲霉间型变种esd C基因的功能分析,菌物学报,34(2):227-234)
Liu R.,Tan Y.M.,Liu Y.X.,and Liu Z.Y.,2013,c DNA cloning and sequence analysis of esd C gene from Aspergillus chevalieri var.intermedius,Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),32(1):36-40(刘荣,谭玉梅,刘永翔,刘作易,2013,谢瓦氏曲霉间型变种(Aspergillus chevalieri var.intermedius)esd C基因全长c DNA克隆与序列分析,基因组学与应用生物学,32(1):36-40)
Liu Z.Y.,Qin J.,an d Wang N.L.,1991,Study of conditions of sporogenesis of Aspergillus chevalieri var.intermedis in Fuzhuan tea,Xinan Nongye Xuebao(Southwest China Journal of Agricultural Sciences),4(1):73-77(刘作易,秦京,李乃亮,1991,茯砖茶“金花”菌-谢瓦氏曲霉间型变种的孢子产生条件,西南农业学报,4(1):73-77)
Ohya Y.,and Anraku Y.,1992,Yeast calmodulin:structural and functional elements essential for the cell cycle,Cell Calcium,13(6-7):445-455
Rodriguez-Caban J.,Gonzalez-Velazquez W.,Perez-Sanchez L.,Gonzalez-Mendez R.,and Rodriguez-del Valle N.,2011,Calcium/calmodulin kinase1 and its relation to thermotolerance and HSP90 in Sporothrix schenckii:an RNAi and yeast two-hybrid study,BMC Microbiol.,11:162
Sanglard D.,Ischer F.,Marchetti O.,Entenza J.,and Bille J.,2003,Calcineurin A of Candida albicans:involvement in antifungal tolerance,cell morphogenesis and virulence,Mol.Microbiol.,48(4):959-976
Soderling T.R.,and Stull J.T.,2001,Structure and regulation of calcium/calmodulin-dependent protein kinases,Chem.Rev.,101(8):2341-2352
Steinbach W.J.,Cramer R.A.,Perfect B.Z.,Asfaw Y.G.,Sauer T.C.,Najvar L.K.,Kirkpatrick W.R.,Patterson T.F.,Benjamin D.K.Jr.,Heitman J.,and Perfect J.R.,2006,Calcineurin controls growth,morphology,and pathogenicity in Aspergillus fumigatus,Eukaryot Cell,5(7):1091-1103
Tan Y.M.,Wang Y.C.,Liu Y.Y.,Sang S.L.,and Liu Z.Y.,2015,Cloning and expression analysis of transcription factor gend stf1 in Erotium cristatum,Junwu Xuebao(Mycosystema),34(1):91-97(谭玉梅,王玉臣,刘永翔,桑石磊,刘作易,2015,冠突散囊菌转录因子stf1基因的克隆及其表达分析,菌物学报,34(1):91-97)
Viaud M.C.,Balhadere P.V.,and Talbot N.J.,2002,A Magnaporthe grisea cyclophilin acts as a virulence determinant during plant infection,Plant Cell,14(4):917-930
Wang Y.C.,Tan Y.M.,Liu Y.X.,and Liu Z.Y.,2013,Full-length cloning and sequence analysis of a c DNA encoding flb A from Aspergillus chevalieri,Xinan Nongye Xuebao(Southwest China Journal of Agricultural Sciences),26(1):358-362(王玉臣,谭玉梅,刘永翔,刘作易,2013,谢瓦氏曲霉间型变种flb A基因c DNA的全长克隆及序列分析,西南农业学报,26(1):358-362)
Warwar V.,and Dickman M.B.,1996,Effects of calcium and calmodulin on spore germination and appressorium development in Colletotrichum trifolii,42(10):74-79
Yu C.F.,Li X.X.,Tan Y.M.,Liu Y.X.,and Liu Z.Y.,2013,Cloning and sequence analysis is of the full length nsd DDNA in Aspergillus chevalieri var.intermedius,Guizhou Nongye Kexue(Guizhou Agricultural Sciences),41(9):33-36(余春芳,李孝霞,谭玉梅,刘永翔,刘作易,2013,谢瓦氏曲霉间型变种nsd D基因全长克隆及序列分析,贵州农业科学,41(9):33-36)
基本信息:
DOI：10.13417/j.gab.036.004663
中图分类号:Q78;Q933
引用信息:
[1]任秀秀,余芳,谭玉梅,等.冠突曲霉钙调素cam基因的克隆及表达分析[J].基因组学与应用生物学,2017,36(11):4663-4669.DOI:10.13417/j.gab.036.004663.
基金信息:
贵州省科技计划项目(黔科合基础[2016]1056);; 贵州省科学技术基金项目(黔科合LH字[2014]7541号)和贵州省科学技术基金项目(黔科合LH字[2014]7693号)共同资助
2017-11-25
2017-11-25