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长链非编码RNA LINC00324在急性髓系白血病(acute myeloid leukemia, AML)发生发展中的作用尚不明确。为探讨AML患者LINC00324表达水平与染色体核型及预后的关系，首先采用实时荧光定量PCR法检测46例AML患者LINC00324的表达水平，并分析其与染色体核型及基因变化的关系；其次利用cBioPortal数据库分析173名AML患者LINC00324水平与各临床指标的关系。实验结果显示，AML患者LINC00324的表达水平明显降低(P<0.001)，尤其在预后不良组显著下调(P<0.05)；利用cBioPortal数据库分析也表明LINC00324水平在预后中等组和预后不良组中均显著下调(P<0.01)，且预后不良组LINC00324水平更低(P<0.05)。在预后不良组中，单体核型患者LINC00324表达水平比非单体核型患者明显降低(P<0.05)。此外，LINC00324表达水平在FLT3-ITD阳性患者、M0、M1、M2型患者中明显下调(P<0.05);LINC00324水平与WT1表达量、C-KIT表达量和骨髓原始细胞数目均呈显著负相关(P<0.001)。LINC00324可能参与AML的发生和发展，有望作为AML患者预后评估的潜在指标。
Abstract：The functional role of long-chain non-coding RNA LINC00324 in the genesis and development of acute myeloid leukemia(AML) remains unknown. To investigated the relationship between genome-wide expression level of LINC00324 and karyotype and prognosis in AML patients, firstly, real-time fluorescence quantitative PCR was carried out to detect the expression level of LINC00324 in 46 patients, and its relationship with chromosome karyotype and gene changes was analyzed. Secondly, cBioPortal database was utilized to analyze the relationship between LINC00324 expression level and clinical indicators in 173 AML patients. Experimental results exhibited that the expression level of LINC00324 was significantly reduced(P<0.001) in AML patients, particularly in the poor prognosis group(P <0.05); Data obtained from cBioPortal database also indicated that the level of LINC-00324 was significantly reduced in both the medium prognosis group and the poor prognosis group(P<0.01), and the poor prognosis group LINC00324 level was lower(P<0.05). Furthermore, in the group with poor prognosis, LINC00324 expression level in patients with monosomal karyotype was lower than that of patients with non-monosomal karyotype(P<0.05). Moreover, LINC00324 expression level was significantly down-regulated in FLT3-ITD positive patients, M0, M1, and M2 patients(P<0.05). Additionally, data also showed LINC00324 levels were negatively correlated with WT1 expression, C-KIT expression, and the number of bone marrow blasts(P<0.001). The results indicate that LINC00324 may be involved in the occurrence and development of AML, and is expected to be a potential indicator for the prognostic evaluation of AML patients.
Anelli L.,Pasciolla C.,Zagaria A.,Specchia G.,and Albano F.,2017,Monosomal karyotype in myeloid neoplasias:a literature review,Onco Targets Ther.,10:2163-2171.
Behrens K.,Maul K.,Tekin N.,Kriebitzsch N.,Indenbirken D.,Prassolov V.,Müller U.,Serve H.,Cammenga J.,and Stocking C.,2017,RUNX1 cooperates with FLT3-ITD to induce leukemia,J.Exp.Med.,214(3):737-752.
Borrow J.,Dyer S.A.,Akiki S.,and Griffiths M.J.,2019,Terminal deoxynucleotidyl transferase promotes acute myeloid leukemia by priming FLT3-ITD replication slippage,Blood,134(25):2281-2290.
Gupta M.,Mahapatra M.,and Saxena R.,2019,Cytogenetics'impact on the prognosis of acute myeloid leukemia,J.Lab.Physicians,11(2):133-137.
Gao X.N.,Lin J.,Gao L.,Deng A.L.,Lu X.L.,Li Y.H.,Wang L.L.,and Yu L.,2015,High expression of C-kit m RNA predicts unfavorable outcome in adult patients with t (8;21) acute myeloid leukemia,PLoS ONE,10(4):e0124241.
Liu L.,Xu H.,Chen Z.M.,Lou J.Y.,Wang H.P.,and Jin J.,2019,Analysis of age-specific cytogenetic changes among 515patients withacute myeloid leukemia,Zhonghua Yixue Yichuanxue Zazhi (Chinese Journal of Medical Genetics),36(6):552-555.(刘林,徐欢,陈志妹,楼基余,王焕萍,金洁,2019,515例急性髓系白血病患者年龄特异的细胞遗传学分析,中华医学遗传学杂志,36(6):552-555.)
Li Z.,Wei H.,Lin D.,Zhou C.L.,Liu B.C.,Wang Y.,Liu K.Q.,Li W.,Gong B.F.,Wei S.N.,Zhang G.J.,Zhao X.L.,Li Y.,Liu Y.T.,Gong X.Y.,Gu R.X.,Qiu S.W.,Mi Y.C.,and Wang J.X.,2016,Cytogentic and prognostic characteristic of acute myeloid leukemia with monosomal karyotype,Zhonghua Xueyexue Zazhi (Chinese Journal of Hematology),37(5):366-371.(李哲,魏辉,林冬,周春林,刘兵城,王迎,刘凯奇,李巍,宫本法,魏述宁,张广吉,赵邢力，李艳,刘云涛,弓晓媛,顾闰夏,邱少伟,秘营昌,王建祥,2016,单体核型急性髓系白血病的细胞遗传学和预后特点,中华血液学杂志,37(5):366-371.)
Liu H.S.,Wang X.N.,Zhang H.L.,Wang J.C.,Chen Y.,Ma T.T.,Shi J.,Kang Y.,Xi J.Y.,Wang M.C.,and Zhang M.,2019,Dynamic changes in the level of WT1 as an MRD marker to predict the therapeutic outcome of patients with AMLwith and without allogeneic stem cell transplantation,Mol.Med.Rep.,20(3):2426-2432.
Leukemia&Lymphoma Group,Chinese Society of Hema,2017,Chinese guidelines for diagnosis and treatment of adult acute myeloid leukemia (not APL)(2017),Zhonghua Xueyexue Zazhi (Chinese Journal of Hematology),38(3):177-182.(中华医学会血液学分会白血病淋巴瘤学组,2017,成人急性髓系白血病(非急性早幼粒细胞白血病)中国诊疗指南(2017年版),中华血液学杂志,38(3):177-182.)
Luan Y.Y.,Xu J.Q.,Huang B.H.,Liu X.Q.,Liu Y.H.,Chen L.M.,and Chu X.X.,2015,Prognostic significance of monosomal karyotype in acute myeloid leukemia,Zhonghua Xueyexue Zazhi (Chinese Journal of Hematology),36(4):286-290.(栾彦彦,徐俊卿,黄葆华,刘小倩,刘英慧,陈丽明,初晓霞,2015,单体核型在急性髓系白血病中的预后意义,中华血液学杂志,36(4):286-290.)
Lagunas-Rangel F.A.,and Chávez-Valencia V.,2017,FLT3-ITDand its current role in acute myeloid leukaemia,Med.Oncol.,34(6):114.
Miao L.,Zhao J.J.,Jia T.,Mao J.P.,Zhu Y.X.,Wei J.F.,Xue L.G.,Zhao L.D.,and Wang Y.,2020,LncRNA HCP5 promotes LAML progression via PSMB8-mediated PI3K/AKTpathway activation,Naunyn Schmiedebergs Arch.Pharmacol.,393(6):1025-1032.
Moarii M.,and Papaemmanuil E.,2017,Classification and risk assessment in AML:integrating cytogenetics and molecular profiling,Hematology Am.Soc.Hematol.Educ.Program,2017(1):37-44.
Sun C.M.,Luan S.P.,Zhang G.L.,Wang N.,Shao H.Y.,and Luan C.F.,2017,CEBPA-mediated upregulation of the lncRNAPLIN2 promotes the development of chronic myelogenous leukemia via the GSK3 and Wnt/β-catenin signaling pathways,Am.J.Cancer Res.,7(5):1054-1067.
Sun L.Y.,Li X.J.,Sun Y.M.,Huang W.,Fang K.,Han C.,Chen Z.H.,Luo X.Q.,Chen Y.Q.,and Wang W.T.,2018,LncRNAANRIL regulates AML development through modulating the glucose metabolism pathway of AdipoR1/AMPK/SIRT1,Mol.Cancer,17(1):127.
Xu J.Q.,Huang B.H.,Liu X.Q.,Zhang Y.F.,Liu Y.H.,Chen L.M.,Luan Y.Y.,Li N.N.,and Chu X.X.,2017,Poor prognosis in acute myeloid leukemia patients with monosomal karyotypes,Turk.J.Haematol.,34(2):126-130.
Yu X.,Li Z.,Zheng H.,Chan M.T.,and Wu W.K.,2017,NEAT1:a novel cancer-related long non-coding RNA,Cell Prolif.,50(2):e12329.
Zeng H.J.,Wang L.L.,and Wang S.H.,2017,Advances in the study of long non-coding RNA in the therapy resistance of breast cancer,Yixue Yanjiusheng Xuebao (Journal of Medical Postgraduates),30(12):1340-1344.(曾慧娟,王璐璐,王少华,2017,长链非编码RAN与乳腺癌治疗耐药相关的研究进展,医学研究生学报,30(12):1340-1344.)
Zou Z.G.,Ma T.S.,He X.Z.,Zhou J.X.,Ma H.W.,Xie M.,Liu Y.H.,Lu D.,Di S.H.,and Zhang Z.H.,2018,Long intergenic non-coding RNA 00324 promotes gastric cancer cell proliferation via binding with HuR and stabilizing FAM83B expression,Cell Death Dis.,9(7):717.
Zheng Y.,Liu L.,and Shukla G.C.,2017,A comprehensive review of web-based non-coding RNA resources for cancer research,Cancer Lett.,407:1-8.
Zhou Z.Y.,Xiang Y.,Yuan S.,Wu L.,Wu N.,Xie W.J.,Xu B.,Li C.Y.,Zhang Y.,Ma X.Y.,Cai T.J.,Yu Z.B.,Bai L.,and Li Y.F.,2019,Effect of long non-coding RNA LINC00324on biological functions of non-small cell lung cancer cells,Disan Junyi Daxue Xuebao (Journal of Third Military Medical University),41(4):331-338.(周征宇,向颖,袁帅,吴龙,邬娜,谢薇佳,许斌,李成英,张耀,马翔宇,蔡同建,余祖滨,白莉,李亚斐,2019,长链非编码RNA LINC00324对非小细胞肺癌细胞生物学功能的影响,第三军医大学学报,41(4):331-338.)
基本信息:
DOI：10.13417/j.gab.040.002837
中图分类号:R733.71
引用信息:
[1]谢佳莹,周吉成,蓝玥,等.急性髓系白血病患者LINC00324表达水平与染色体核型及预后的关系[J].基因组学与应用生物学,2021,40(Z2):2837-2844.DOI:10.13417/j.gab.040.002837.
基金信息:
国家自然科学基金(81560434); 广西自然科学基金(2017GXNSFAA198151)共同资助
2021-08-25
2021-08-25