| 440 | 6 | 50 |
| 下载次数 | 被引频次 | 阅读次数 |
植物促生长细菌(plant growth-promoting bacteria,PGPB)在植物修复重金属污染土壤的过程中能够有助于植物生长,从而提高植物的重金属污染土壤的修复能力。作为在重金属污染环境中应用的PGPB首先必须具有重金属抗性。镉是一种毒性很高的重金属,也是常见的环境污染因子之一。在植物中,镉抑制根和茎的生长,影响营养吸收和内环境稳定。现已知的PGPB的作用机制之一是产生植物生长激素如吲哚乙酸(indole acetic acid,IAA)和铁载体(siderophore)。本研究从土壤中筛选获得5株具有镉抗性(>1.55 mmol/L)且产IAA和铁载体的细菌,这5株菌被编号为DJY、TK-2、TK-6、WM-1、PZ-23。在含镉培养基上,所有5种菌对芥菜根均有不同程度的促生长作用,其中TK-2对芥菜幼苗根萌发和下胚轴延伸的促进效果最为显著;促生效应并不由单一因素决定的,而是多种促生因素的统一协调作用的结果。
Abstract：Plant growth-promoting bacteria(PGPB) are helpful to plant growth during phytoremediation of heavy metal-polluted soils, and can therefore enhance the ability of remediation of heavy metal-polluted soils by plants.However, the PGPB applied in the heavy-metal-polluted environments should first have a resistance to heavy metals. Cadmium is one of common environmental pollution factors which is highly toxic. Cadmium can inhibit the growth of root and stem in plants and affect nutrient absorption and homeostasis. One of the known mechanisms of plant growth promoting effect of the PGPB is to produce plant growth hormone such as IAA and siderophore. Five strains of cadmium-resistant(>1.55 mmol/L) and IAA-producing, siderophores-producing bacteria were isolated from the soil, which were numbered DJY, TK-2, TK-6, WM-1 and PZ-23. All five kinds of bacteria have different levels of growth-promoting effect on roots of mustard grown on the cadmium-containing medium, of which TK-2 had the best effect of promoting elongation of roots and hypocotyls of plants.Growth-promoting effects by bacteria result from coordinated effects of multiple factors instead of single factor.
Belimov A.A.,Hontzeas N.,Safronova V.I.,Demchinskaya S.V.,Piluzza G.,Bullitta S.,and Glick B.R.,2005,Cadmium-tolerant plant growth-promoting bacteria assoeiated with theroots of Indian mustard(Brassica juncea L.Czern.),Siol.Biology and Biochemistry,37(2):241-250
Buchannan R.E.,and Gibbons N.E.,eds.,The Institute of Microbiology Chinese Academy of Sciences,trans.,1984,Bergey'smanual of determinative bacteriology,Science Press,Beijing,China,pp.729-736
Burd G.I.,Dixon D.G.,and Glick B.R.,1998,A plant grow thpromoting bacterium that decreases nickel toxicity inseedlings,Applied and Environmental Microbiology,64(10):3663-3668
Cabrera F.,Clemente L.,Barrientos E.D.,Lopez R.,and MurilloJ.M.,1999,Heavy metal pollution of soils affected by the Guadiamar toxic flood,Science of the Total Environment,242:117-129
Campbell P.G.C.,2006,Cadmium-A priority pollutant,Environmental Chemistry,3:387-388
Chen L.P.,Zhang L.J.,and Fu H.,2010,Progress in research inferritin,Caoaye Xuebao(Acta Prataculturae Sinica),19(6):263-271(陈丽萍,张丽静,傅华,2010,植物铁蛋白的研究进展,草业学报,19(6):263-271)
Dell'Amico E.,Cavalca L.,and Andreoni V.,2008,Improvement of Brassica napus growth under cadmium stress by cadmium-resistant rhizobacteria,Soil Biology&Biochemistry,40(1):74-84
Dwork in M.,and Foster J.,1958,Experiments with some microorganisms which utilize ethane and hydrogen,Journal of Bacteriology,75:592-601
Fu J.R.,1974,The regulation of photo hormone on seed doemancy,Zhongshan Daxue Xuebao(Zirankexue Ban)(Acta Scientiarum Naturalium Universitatis Sunyatseni),3:99-106(傅家瑞,1974,植物激素在种子休眠中的调节作用,中山大学学报(自然科学版),3:99-106)
Gamalero E.,Lingua G.,Berta G.,and Glick B.R.,2009,Beneficial role of plant growth promoting bacteria and arbuscular mycorrhizal fungi on plant responses to heavy metal stress,Canadian Journal of Microbiology,55(5):501-514
Glickmann E.,and Dessaux Y.,1995,A critical examination of the specificity of the Salkowski reagent for in dolic compounds produced by phytopathogenic bacteria,Applied and Environmental Microbiology,61(2):793-796
Hayes W.J.,Chaudhry T.M.,Buckney R.T.,and Khan A.G.,2003,Phytoaccu mulation of trace metals at the sunny cornermine,New South Wales,with suggestions for a possible remediation strategy,Phytoaccu mulation at the Sunny Cornermine,9:69-82
Huo W.,and Cai Q.S.,2010,The advance of the enhancement of plant heavy metal resistance by plant growth-promote bacteria,Weisheng wuxue Tongbao(Microbiology China),37(9):1374-1378(霍伟,蔡庆生,2010,植物促生菌提高植物重金属耐受性研究进展,微生物学通报,37(9):1374-1378)
Jrup L.,2003,Hazards of heavy metal contamination,British Medical Bulletin,68(1):167-182
Kloepper J.W.,Leong J.,Teintze M.,and Schroth M.N.,1980,Pseudomonas sider ophores:A mechanism explaining disease-suppressive soils,Current Microbiology,4:317-320
Lasat M.M.,2002,Phytoextraction of toxic metals:A review of biological mechanisms,J.Environ.Qual.31:109-120
Li C.X.,Shao Y.,and Jiang L.N.,2008,Biostatistics,Science Press,Beijing,China,pp.92-97(李春喜,邵云,姜丽娜,2008,生物统计学,科学出版社,中国,北京,pp.92-97)
Liu S.C.,Xiao L.T.,Liao B.H.,Lu X.D.,Zhao W.K.,Kuang F.C.,and Tong J.H.,2005,Effects of lead stress on dynamic accumulation of endogenous phytohormones in cucumberseedlings,Hunan Nongye Daxue Xuebao(Zirankexue Ban)(Joumal of Hunan Agricultural University(Natural Sciences)),31(5):510-513(刘素纯,萧浪涛,廖柏寒,鲁旭东,赵文魁,匡逢春,童建华,2005,铅胁迫对黄瓜幼苗内源激素积累动态的影晌,湖南农业大学学报(自然科学版),31(5):510-513)
Mabrouk A.,and Sharkawy E.L.,2004,Cassava biology and physiology,Plant Molecular Biology,56:481-501
Rajkumar M.,Ae N.,Prasad M.N.,and Freitas H.,2010,Potential of siderophore-producing bacteria for improving heavy metal phytoex traction,Trends Biotechnol.,28:142-149
Sam brook J.,Fritsch E.F.,and Maniatis T.,1989,Molecularcloning:A laboratory manual,2ndedtion,Cold Spring Harbor Laboratory Press,Cold Spring Harbor,New York,pp.1-100
Sayyed R.Z.,Badgujar M.D.,Sona wane H.M.,Mhaske M.M.,and Chincholkar S.B.,2005,Production of microbial ironchelators(siderophores)by fluorescent Pseudomonads,Indian Journal of Biotechnology 4:484-490
Schwyn B.,and Neilands J.B.,1987,Universal chemical assayfor the detection and determination of siderophores,Analytical Biochemistry,160:47-56
Wuana R.A.,and Okieimen F.E.,2011,Heavy metals in contaminated soils:A review of sources,chemistry,risks and besta vailable strategies for remediation,International Scholarly Research Network Ecology,2011:1-20
Zhao Q.,He W.R.,Zhang X.Y.,and Guo H.W.,2010,Genetic and biochemical study of the ethylene signaling in Arabidopsis,Shengming Kexue(Chinese Bulletin of Life Sciences),22(11):1167-1172(赵琼,何文容,张新岩,郭红卫,2010,拟南芥乙烯信号转导机理的遗传学和化学生物学研究,生命科学,22(11):1167-1172)
基本信息:
DOI：10.13417/j.gab.033.000888
中图分类号:X17;Q93
引用信息:
[1]田会会,蔡春响,李莉,等.抗镉并且产吲哚乙酸的细菌的筛选鉴定及其植物促生作用[J].基因组学与应用生物学,2014,33(04):888-896.DOI:10.13417/j.gab.033.000888.
2014-08-28
2014-08-28